Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins.

1983 ◽  
Vol 18 (6) ◽  
pp. 1399-1404 ◽  
Author(s):  
H W Kim ◽  
R G Wyatt ◽  
B F Fernie ◽  
C D Brandt ◽  
J O Arrobio ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Nasal Secretions ◽  
Syncytial Virus

Respiratory syncytial virus detection in cells and clinical samples by using three new monoclonal antibodies

2013 ◽  
Vol 86 (7) ◽  
pp. 1256-1266 ◽  
Author(s):  
Roberto S. Gómez ◽  
Jorge E. Mora ◽  
Claudia M. Cortés ◽  
Claudia A. Riedel ◽  
Marcela Ferrés ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Clinical Samples ◽  
Syncytial Virus ◽  
In Cells

scholarly journals Quantification of Respiratory Syncytial Virus Polypeptides in Nasal Secretions by Monoclonal Antibodies

1985 ◽  
Vol 66 (8) ◽  
pp. 1705-1714 ◽  
Author(s):  
R. M. Hendry ◽  
E. Godfrey ◽  
L. J. Anderson ◽  
B. F. Fernie ◽  
K. McIntosh
Keyword(s):  
Monoclonal Antibodies ◽  
Respiratory Syncytial Virus ◽  
Nasal Secretions ◽  
Syncytial Virus

scholarly journals Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 175
Author(s):  
Wioleta Białobrzeska ◽  
Daniel Firganek ◽  
Maciej Czerkies ◽  
Tomasz Lipniacki ◽  
Marta Skwarecka ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Characterizations ◽  
Virus Detection ◽  
Electrochemical Immunosensors ◽  
Syncytial Virus ◽  
Screen Printed

This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 105 PFU/mL to 1.5×107 PFU/mL, more than 1.0 × 105 PFU/mL to 1.0 × 107 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 103 PFU/mL, three orders of magnitude lower than 2.85 × 106 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

Beta-Chemokines in Nasal Secretions of Infants with Respiratory Syncytial Virus-Induced Respiratory Infections

2001 ◽  
Vol 15 (2) ◽  
pp. 89-96 ◽  
Author(s):  
Roberto P. Garofalo ◽  
Barbara Olszewska-Pazdrak ◽  
Pearay L. Ogra ◽  
Robert C. Welliver
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Infections ◽  
Nasal Secretions ◽  
Syncytial Virus

Evaluation of Respiratory Syncytial Virus Detection by Rapid Antigen Tests in Childhood

2007 ◽  
Vol 219 (4) ◽  
pp. 212-216 ◽  
Author(s):  
U. Schauer ◽  
G. Ihorst ◽  
A. Rohwedder ◽  
G. Petersen ◽  
R. Berner ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Detection ◽  
Syncytial Virus ◽  
Antigen Tests
Sign in / Sign up

Export Citation Format

Share Document