scholarly journals Agilent 2100 Bioanalyzer for Restriction Fragment Length Polymorphism Analysis of the Campylobacter jejuni Flagellin Gene

2001 ◽  
Vol 39 (2) ◽  
pp. 754-757 ◽  
Author(s):  
I. Nachamkin ◽  
N. J. Panaro ◽  
M. Li ◽  
H. Ung ◽  
P. K. Yuen ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Campylobacter Jejuni ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Flagellin Gene ◽  
Restriction Fragment Length

scholarly journals Competitive Exclusion of HeterologousCampylobacter spp. in Chicks

2001 ◽  
Vol 67 (2) ◽  
pp. 848-851 ◽  
Author(s):  
Hui-Cheng Chen ◽  
Norman J. Stern
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Campylobacter Jejuni ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Competitive Exclusion ◽  
Fragment Length Polymorphism ◽  
Broiler Chicks ◽  
Flagellin Gene ◽  
Restriction Fragment Length

ABSTRACT Chicken and human isolates of Campylobacter jejuniwere used to provide oral challenge of day-old broiler chicks. The isolation ratio of the competing challenge strains was monitored and varied, depending upon the isolates used. A PCR-restriction fragment length polymorphism assay of the flagellin gene (flaA) was used to discriminate between the chick-colonizing isolates. Our observations indicated that the selected C. jejunicolonizers dominated the niche provided by the chicken ceca. Chicken isolates from the flaA type 7 grouping generally had numerical superiority over the human isolates when they were administered in our 1-day-old chick model. Our results suggest that it is possible to use combinations of C. jejuni chicken isolates as a defined bacterial preparation for the competitive exclusion of human-pathogenic C. jejuni in poultry.

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