scholarly journals Comparison of Quantitative Reverse Transcription-PCR to Viral Culture for Assessment of Respiratory Syncytial Virus Shedding

2003 ◽  
Vol 41 (9) ◽  
pp. 4160-4165 ◽  
Author(s):  
A. R. Falsey ◽  
M. A. Formica ◽  
J. J. Treanor ◽  
E. E. Walsh
Keyword(s):  
Respiratory Syncytial Virus ◽  
Reverse Transcription ◽  
Virus Shedding ◽  
Quantitative Reverse Transcription Pcr ◽  
Reverse Transcription Pcr ◽  
Viral Culture ◽  
Syncytial Virus

scholarly journals Evaluation of a Nested Reverse Transcription-PCR Assay Based on the Nucleoprotein Gene for Diagnosis of Spontaneous and Experimental Bovine Respiratory Syncytial Virus Infections

1999 ◽  
Vol 37 (6) ◽  
pp. 1858-1862 ◽  
Author(s):  
Jean-François Valarcher ◽  
Hervé Bourhy ◽  
Jacqueline Gelfi ◽  
François Schelcher
Keyword(s):  
Respiratory Syncytial Virus ◽  
Reverse Transcription ◽  
Lavage Fluid ◽  
Bovine Respiratory Syncytial Virus ◽  
Virus Infections ◽  
Rt Pcr ◽  
Nucleoprotein Gene ◽  
Reverse Transcription Pcr ◽  
Field Samples ◽  
Syncytial Virus

The first nested reverse transcription (RT)-PCR based on the nucleoprotein gene (n RT-PCR-N) of the bovine respiratory syncytial virus (BRSV) has been developed and optimized for the detection of BRSV in bronchoalveolar lavage fluid cells of calves. This test is characterized by a low threshold of detection (0.17 PFU/ml), which is 506 times lower than that obtained by an enzyme immunosorbent assay (EIA) test (RSV TESTPACK ABBOTT). During an experimental infection of 17 immunocompetent calves less than 3 months old, BRSV RNA could be detected up to 13 days after the onset of symptoms whereas isolation in cell culture was possible only up to 5 days. Compiling results obtained by conventional techniques (serology, antigen detection, and culture isolation) for 132 field samples collected from calves with acute respiratory signs revealed that n RT-PCR-N showed the highest diagnostic sensitivity and very good specificity. This n RT-PCR-N with its long period of detection during BRSV infection thus provides a valuable tool for diagnostic and epidemiological purposes.

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