UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

scholarly journals UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion.

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162 ◽  
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

Preparation of a "functional library" of African green monkey DNA fragments which substitute for the processing/polyadenylation signal in the herpes simplex virus type 1 thymidine kinase gene

1983 ◽  
Vol 3 (4) ◽  
pp. 643-653
Author(s):  
G M Santangelo ◽  
C N Cole
Keyword(s):  
Gene Expression ◽  
Thymidine Kinase ◽  
Low Frequency ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Dna Fragments ◽  
Coding Region ◽  
Kinase Gene ◽  
Green Monkey

Fragments of African green monkey (Cercopithecus aethiops) DNA (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (TK, tk) coding region in pTK206/SV010, a gene construct which lacks both copies of the hexanucleotide 5'-AATAAA-3' and contains a simian virus 40 origin of replication, allowing it to replicate in Cos-1 cells. No polyadenylated tk mRNA was detected in Cos-1 cells transfected by pTK206/SV010. The ability of simian DNA fragments to restore tk gene expression was examined by measuring the incorporation of [125I]iododeoxycytidine into DNA in Cos-1 cells transfected by pTK206/SV010 insertion derivatives. tk gene expression was restored by the insertion in 56 of the 67 plasmids analyzed, and the level of expression equaled or exceeded that obtained with the wild-type tk gene in 30 of these. In all plasmids examined that showed restoration of tk gene expression, polyadenylated tk mRNA of discrete size was detected. The sizes of these tk mRNAs were consistent with the existence of processing and polyadenylation signals within the inserted DNA fragments. The frequency with which inserted fragments restored tk gene expression suggests that the minimal signal for processing and polyadenylation is a hexanucleotide (AAUAAA or a similar sequence). LTK- cells were biochemically transformed to TK+ with representative insertion constructs. pTK206/SV010 transformed LTK- cells at a very low frequency; the frequency of transformation with insertion derivatives was 40 to 12,000 times higher.

African Green Monkey Fibroblast Action Morphology during SV40 Infection

1977 ◽  
Vol 32 (5-6) ◽  
pp. 409-412
Author(s):  
Gerhard Brandner ◽  
Myung-Sam Cho
Keyword(s):  
Tumor Antigen ◽  
Actin Filaments ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Skin Fibroblasts ◽  
Focus Formation ◽  
Normal Morphology ◽  
Green Monkey ◽  
Sv40 Infection

Abstract Monkey skin fibroblasts were infected with simian virus 40. Cells that exhibited the viral tumor antigen were found to retain the normal morphology of actin filaments up to six days after infection. However when cells were transformed in terms of focus formation they had lost the normal actin morphology.

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