The Monoheme c Subunit of Respiratory Alternative Complex III Is Not Essential for Electron Transfer to Cytochrome aa 3 in Flavobacterium johnsoniae

Energy conversion is a fundamental process of all organisms, realized by specialized protein complexes, one of which is alternative complex III (ACIII). ACIII is a functional analogue of well-known mitochondrial complex III, but operates according to a different, still unknown mechanism.

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Novel purification of cytochrome c1 from mitochondrial Complex III. Reconstitution of antimycin-insensitive electron transfer with the iron-sulfur protein and cytochrome c1.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)95704-2 ◽

1985 ◽

Vol 260 (28) ◽

pp. 15075-15080

Author(s):

Y Shimomura ◽

M Nishikimi ◽

T Ozawa

Keyword(s):

Electron Transfer ◽

Complex Iii ◽

Mitochondrial Complex ◽

Iron Sulfur ◽

Cytochrome C1 ◽

Sulfur Protein ◽

Iron Sulfur Protein

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Enzymatic activity of the alternative complex III as a menaquinol:auracyanin oxidoreductase in the electron transfer chain ofChloroflexus aurantiacus

FEBS Letters ◽

10.1016/j.febslet.2009.09.022 ◽

2009 ◽

Vol 583 (19) ◽

pp. 3275-3279 ◽

Cited By ~ 28

Author(s):

Xinliu Gao ◽

Yueyong Xin ◽

Robert E. Blankenship

Keyword(s):

Electron Transfer ◽

Enzymatic Activity ◽

Complex Iii ◽

Electron Transfer Chain ◽

Alternative Complex Iii ◽

Transfer Chain

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The Alternative complex III: Properties and possible mechanisms for electron transfer and energy conservation

Biochimica et Biophysica Acta (BBA) - Bioenergetics ◽

10.1016/j.bbabio.2012.05.003 ◽

2012 ◽

Vol 1817 (10) ◽

pp. 1852-1859 ◽

Cited By ~ 32

Author(s):

Patrícia N. Refojo ◽

Miguel Teixeira ◽

Manuela M. Pereira

Keyword(s):

Electron Transfer ◽

Energy Conservation ◽

Complex Iii ◽

Alternative Complex Iii

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Functional analysis and expression of the mono-heme containing cytochrome c subunit of alternative complex III in Chloroflexus aurantiacus

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2013.04.002 ◽

2013 ◽

Vol 535 (2) ◽

pp. 197-204 ◽

Cited By ~ 8

Author(s):

Xinliu Gao ◽

Erica Wunderlich Majumder ◽

Yisheng Kang ◽

Hai Yue ◽

Robert E. Blankenship

Keyword(s):

Functional Analysis ◽

Cytochrome C ◽

Complex Iii ◽

Chloroflexus Aurantiacus ◽

C Subunit ◽

Alternative Complex Iii

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The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus

Biological Chemistry ◽

10.1515/hsz-2016-0323 ◽

2017 ◽

Vol 398 (9) ◽

pp. 1037-1044 ◽

Cited By ~ 3

Author(s):

Patrícia N. Refojo ◽

Filipa Calisto ◽

Miguel A. Ribeiro ◽

Miguel Teixeira ◽

Manuela M. Pereira

Keyword(s):

Cytochrome C ◽

Electron Donor ◽

Complex Iii ◽

Rhodothermus Marinus ◽

Oxidoreductase Activity ◽

C Subunit ◽

Respiratory Chains ◽

Oxygen Reductase ◽

Alternative Complex Iii

Abstract Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.

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