The Mo mineralization of the Kayizi deposit occurs in the edge of a granodiorite intrusion, and consists of two mineral belts, in which the biggest ore body is 400 m long and 140 m wide. The ore is dominated by disseminated and spot-like molybdenite, with its grade of 0.04%~1.53%, and some up to 10%~15%. The country rock alteration displays distinctive porphyry deposit alteration assemblages, including K-feldspathisation, pyrite-sericitization, and propylition. The petrochemical of the Mo-bearing granodiorites suggests the petrogenesis associated with subduction. The Re-Os model ages of 254.4±1.9 Ma, 257.0±3.4 Ma, and 258.5±2.0 Ma, yielded from three molybdenites, support that the mineralization occurred in the late Permian, whilst the deposit was tectonically in the late Paleozoic rift basin. Therefore, we considered in this paper that the Kayizi porphyry Mo deposit may have been formed as a result of the paleo Tethys subduction to the Tarim plate.