Clinical outcomes of xeno-free allogeneic cultivated limbal epithelial transplantation for bilateral limbal stem cell deficiency

2012 ◽  
Vol 96 (12) ◽  
pp. 1504-1509 ◽  
Author(s):  
Sayan Basu ◽  
Mark M Fernandez ◽  
Sujata Das ◽  
Subhash Gaddipati ◽  
Geeta K Vemuganti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Clinical Outcomes ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell ◽  
Cultivated Limbal Epithelial Transplantation

Unilateral Partial Limbal Stem Cell Deficiency: Contralateral Versus Ipsilateral Autologous Cultivated Limbal Epithelial Transplantation

2014 ◽  
Vol 157 (3) ◽  
pp. 584-590.e2 ◽  
Author(s):  
Jayesh Vazirani ◽  
Sayan Basu ◽  
Hemal Kenia ◽  
Md Hasnat Ali ◽  
Santhosh Kacham ◽  
...  
Keyword(s):  
Stem Cell ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell ◽  
Cultivated Limbal Epithelial Transplantation

scholarly journals Midterm outcomes of penetrating keratoplasty following allogeneic cultivated limbal epithelial transplantation in patients with bilateral limbal stem cell deficiency

2021 ◽  
Vol 14 (11) ◽  
pp. 1690-1699
Author(s):  
Jun-Fa Xue ◽  
◽  
Ya-Ni Wang ◽  
Chen Chen ◽  
Ru-Fei Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Graft Survival ◽  
Graft Rejection ◽  
Ocular Surface ◽  
Penetrating Keratoplasty ◽  
Limbal Stem Cell Deficiency ◽  
Time Interval ◽  
Case Series Study ◽  
Limbal Stem Cell ◽  
Cultivated Limbal Epithelial Transplantation

AIM: To evaluate the midterm outcomes of penetrating keratoplasty (PK) following allogeneic cultivated limbal epithelial transplantation (CLET) for bilateral total limbal stem cell deficiency (LSCD). METHODS: Ten patients (10 eyes) with bilateral LSCD were enrolled in this prospective noncomparative case series study. Each participant underwent PK approximately 6mo after a CLET. Topical tacrolimus, topical and systemic steroids, and oral ciclosporin were administered postoperatively. Best-corrected visual acuity (BCVA), intraocular pressure (IOP), ocular surface grading scores (OSS), corneal graft epithelial rehabilitation, persistent epithelial defect (PED), immunological rejection, and graft survival rate were assessed. RESULTS: The time interval between PK and allogeneic CLET was 6.90±1.29 (6-10)mo. BCVA improved from 2.46±0.32 logMAR preoperatively to 0.77±0.55 logMAR post-PK (P<0.001). Kaplan-Meier analysis of mean graft survival revealed graft survival rates of 100% at 12 and 24mo and 80.0% at 36mo. PEDs appeared in 5 eyes at different periods post-PK, and graft rejection occurred in 4 eyes. The total OSS decreased from 12.4±4.4 before allogeneic CLET to 1.4±1.51 after PK. CONCLUSION: A sequential therapy design of PK following allogeneic CLET can maintain a stable ocular surface with improved BCVA despite the relatively high graft rejection rate.

Clinical Outcomes of Modified Simple Limbal Epithelial Transplantation for Limbal Stem Cell Deficiency in Chinese Population: A Retrospective Case Series

2021 ◽  
Author(s):  
Yinghui Wang ◽  
Xiaodan Hu ◽  
Ke Yang ◽  
Yang Zhang ◽  
Shijing Deng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Clinical Outcomes ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Successful Outcome ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

Abstract Objective To report clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD). Methods 13 patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalisation, vascularisation, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively. In 2 cases, in vivo confocal microscopy (IVCM) and impression cytology (IC) were performed to assess the proliferation and degeneration of limbal tissue. Results At a postoperative follow-up of 6.5 ± 5.3 (range, 2–20) months, 10 (10/13, 76.92%) eyes maintained a successful outcome. The grades of symblepharon, corneal conjunctivalisation, vascularisation, and opacification were significantly improved after SLET (P < 0.05). Two-line improvement in VA was found in 6 (6/10, 60%) eyes of the successful cases. Recurrence of LSCD occurred in 3 (3/13, 23.08%) eyes and conjunctival cyst occurred in 1 patient. After SLET, morphology and structure of corneal epithelial cells and epithelial transition around the limbal tissue fragments were detected by IVCM and IC. Conclusions Our findings suggest that the SLET is a safe and effective technique for the treatment of LSCD. The corneal stroma and hAM can provide protection and nutrition for the limbal stem cells (LSCs) without negatively influencing clinical outcomes. IVCM and IC after SLET can evaluate the effectiveness of surgery and the transition of LSCs and corneal epithelial cells.

Clinical outcomes and complications of fluid-filled scleral lens devices for the management of limbal stem cell deficiency

2021 ◽  
pp. 101528
Author(s):  
Clémence Bonnet ◽  
Andrew Lee ◽  
Vivian P. Shibayama ◽  
Chi-Hong Tseng ◽  
Sophie X. Deng
Keyword(s):  
Stem Cell ◽  
Clinical Outcomes ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell

scholarly journals Long-Term Outcomes of Cultivated Limbal Epithelial Transplantation: Evaluation and Comparison of Results in Children and Adults

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Anita Ganger ◽  
M. Vanathi ◽  
Sujata Mohanty ◽  
Radhika Tandon
Keyword(s):  
Stem Cell ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Long Term Results ◽  
Retrospective Case ◽  
Visual Improvement ◽  
Limbal Stem Cell ◽  
Anatomical Success ◽  
Cultivated Limbal Epithelial Transplantation

Purpose.To compare the long-term clinical outcomes of cultivated limbal epithelial transplantation (CLET) in children and adults with limbal stem cell deficiency.Design. Retrospective case series.Methods. Case records of patients with limbal stem cell deficiency (LSCD) who underwent CLET from April 2004 to December 2014 were studied. Outcome measures were compared in terms of anatomical success and visual improvement. Parameters for total anatomical success were avascular, epithelized, and clinically stable corneal surface without conjunctivalization, whereas partial anatomical success was considered when mild vascularization (sparing centre of cornea) and mild conjunctivalization were noted along with complete epithelization.Results. A total of 62 cases underwent the CLET procedure: 38 (61.3%) were children and 24 (38.7%) were adults. Patients with unilateral LSCD (33 children and 21 adults) had autografts and those with bilateral LSCD (5 children and 3 adults) had allografts. Amongst the 54 autografts partial and total anatomical success were noted in 21.2% and 66.6% children, respectively, and 19.0% and 80.9% in adults, respectively (pvalue 0.23). Visual improvement of 1 line and ≥2 lines was seen in 57.5% and 21.2% children, respectively, and 38% and 38% in adults, respectively (pvalue 0.31).Conclusion. Cultivated limbal epithelial transplantation gives good long-term results in patients with LSCD and the outcomes are comparable in children and adults.

scholarly journals Midterm Outcomes of Penetrating Keratoplasty Following Allogeneic Cultivated Limbal Epithelial Transplantation in Patients with Bilateral Limbal Stem Cell Deficiency

2021 ◽  
Author(s):  
Junfa Xue ◽  
Dongfang Li ◽  
Yani Wang ◽  
Chen Chen ◽  
Rufei Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Graft Survival ◽  
Graft Rejection ◽  
Ocular Surface ◽  
Penetrating Keratoplasty ◽  
Limbal Stem Cell Deficiency ◽  
Topical Tacrolimus ◽  
Case Series Study ◽  
Limbal Stem Cell ◽  
Cultivated Limbal Epithelial Transplantation

Abstract To evaluate the midterm outcomes of penetrating keratoplasty (PK) following allogeneic cultivated limbal epithelial transplantation (CLET) for bilateral total limbal stem cell deficiency (LSCD)，ten patients (10 eyes) with bilateral LSCD were enrolled in this prospective noncomparative case series study. Each participant received a PK approximately 6 months after a CLET. Topical tacrolimus, topical and systemic steroids, and oral ciclosporin were given postoperatively. Best-corrected visual acuity (BCVA), intraocular pressure (IOP), ocular surface grading scores (OSS), corneal graft epithelial rehabilitation, persistent epithelial defect (PED), immunological rejection, and graft survival rate were assessed. The time interval between PK and allogeneic CLET was 6.90 ± 1.29 (6-10) months. BCVA developed from 2.46±0.32 LogMAR preoperatively to 0.77±0.55 LogMAR post-PK (P<0.001). Kaplan-Meier mean graft survival was 100% at 12, 24 months, 80.0% at 36 months. PEDs appeared in 5 eyes at different periods post-PK, while graft rejection post-PK occurred in 4 eyes. Total OSS dropped from 12.4±4.4 before allogeneic CLET to 1.4±1.5 after PK. The sequential therapy of PK following allogeneic CLET can maintain a stable ocular surface with improved BCVA despite a relatively high graft rejection rate. Topical tacrolimus is effective in promoting PK survival post-CLET compared with previous reports.

Sign in / Sign up

Export Citation Format

Share Document