A restriction fragment length polymorphism based linkage map of a diploid Avena recombinant inbred line population

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 192-204 ◽  
Author(s):  
C.A. Kremer ◽  
M. Lee ◽  
J.B. Holland
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Restriction Fragment ◽  
Inbred Line ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Recombinant Inbred Line Population ◽  
Recombinant Inbred ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

A restriction fragment length polymorphism based linkage map of a diploid Avena recombinant inbred line population

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 192-204 ◽  
Author(s):  
C A Kremer ◽  
M Lee ◽  
J B Holland
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Recombinant Inbred ◽  
Fragment Length Polymorphism ◽  
Grass Species ◽  
Disease Resistance Gene ◽  
Restriction Fragment Length

A population of 100 F6-derived recombinant inbred lines was developed from the cross of two diploid (2n = 14) Avena accessions, CI3815 (A. strigosa) and CI1994 (A. wiestii). Restriction fragment length polymorphism (RFLP) probes previously mapped in other grass species were used to develop a framework linkage map suitable for comparative genetics. Nine linkage groups were identified among the 181 loci mapped, with an average interlocus distance of 5 cM, and a total genetic map length of 880 cM. A cluster of five tightly linked crown rust resistance genes (Pca) was localized on the map, as were five loci identified by disease resistance gene analogs from maize, sorghum, and wheat. None of the five loci identified by the gene analogs were linked to the Pca locus. The linkage map was compared with previously published diploid and hexaploid linkage maps in an attempt to identify homologous or homoeologous chromosomes between populations. Locus orders and linkage relationships were poorly conserved between the A. strigosa × A. wiestii map and other Avena maps. In spite of mapping complications due to duplications within a basic genome as well as the allopolyploid constitution of many Avena species, such map comparisons within Avena provide further evidence of substantial chromosomal rearrangement between species within Avena.Key words: grasses, oat, genetic mapping, homoeology.

scholarly journals Linkage map of restriction fragment length polymorphism loci in rice.

1991 ◽  
Vol 41 (4) ◽  
pp. 665-670 ◽  
Author(s):  
Akira SAITO ◽  
Masahiro YANO ◽  
Naoki KISHIMOTO ◽  
Masahiro NAKAGAWA ◽  
Atsushi YOSHIMURA ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana

1989 ◽  
Vol 1 (7) ◽  
pp. 699 ◽  
Author(s):  
Hong-Gil Nam ◽  
Jerome Giraudat ◽  
Bart den Boer ◽  
Francis Moonan ◽  
William D. B. Loos ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Construction of a high-density linkage map of Italian ryegrass (Lolium multiflorum Lam.) using restriction fragment length polymorphism, amplified fragment length polymorphism, and telomeric repeat associated sequence markers

Genome ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Maiko Inoue ◽  
Zhensheng Gao ◽  
Mariko Hirata ◽  
Masahiro Fujimori ◽  
Hongwei Cai
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Lolium Multiflorum ◽  
Telomeric Repeat ◽  
Italian Ryegrass ◽  
Restriction Fragment Length

To construct a high-density molecular linkage map of Italian ryegrass (Lolium multiflorum Lam.), we used a two-way pseudo-testcross F1 population consisting of 82 individuals to analyze three types of markers: restriction fragment length polymorphism markers, which we detected by using genomic probes from Italian ryegrass as well as heterologous anchor probes from other species belonging to the Poaceae family, amplified fragment length polymorphism markers, which we detected by using PstI/MseI primer combinations, and telomeric repeat associated sequence markers. Of the restriction fragment length polymorphism probes that we generated from a PstI genomic library, 74% (239 of 323) of randomly selected probes detected hybridization patterns consistent with single-copy or low-copy genetic locus status in the screening. The 385 (mostly restriction fragment length polymorphism) markers that we selected from the 1226 original markers were grouped into seven linkage groups. The maps cover 1244.4 cM, with an average of 3.7 cM between markers. This information will prove useful for gene targeting, quantitative trait loci mapping, and marker-assisted selection in Italian ryegrass.Key words: linkage map, molecular markers, synteny, Italian ryegrass, Lolium multiflorum Lam.

A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn. campestris)

Genome ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 746-757 ◽  
Author(s):  
Y.-S. Chyi ◽  
M. E. Hoenecke ◽  
J. L. Sernyk
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Linkage Map ◽  
Brassica Rapa ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Genome Structure ◽  
Restriction Fragment Length

A genetic linkage map has been constructed based on restriction fragment length polymorphism DNA markers for Brassica rapa L. using a segregating F2 progeny from a cross between the yellow sarson type 'R500' and the canola-type 'Horizon'. The map contains 360 marker loci detected by 269 genomic clones derived from a PstI library of 'Westar' (Brassica napus L.). The map consists of 10 linkage groups, covering a total of 1876 recombination units. The occurrence of substantial genome rearrangement during the evolution of B. rapa is evident from (i) a large number of homologous duplicate sequences either at genetically adjacent locations or in different linkage groups and (ii) a number of missing sequences in one or the other parental genome, detected as null alleles. Divergence of the parental genotypes is further supported by the frequency of loci with skewed allele segregation ratios. Comparison of molecular and cytogenetic data on genome structure is discussed. The probe set and map are being used to facilitate rapeseed breeding.Key words: Brassica campestris, Brassica rapa, genome structure, linkage map, restriction fragment length polymorphism.

Sign in / Sign up

Export Citation Format

Share Document