Modulation of Microsomal Cytochrome P450 by Scutellariae Radix and Gentianae Scabrae Radix in Rat Liver

1996 ◽  
Vol 24 (01) ◽  
pp. 19-29 ◽  
Author(s):  
Jaw-Jou Kang ◽  
Yi-Chien Chen ◽  
Wei-Chung Kuo ◽  
Terri Chen ◽  
Yu-Wen Cheng ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Medicinal Herbs ◽  
Hydroxylase Activity ◽  
Microsomal Cytochrome ◽  
Aniline Hydroxylase ◽  
Scutellariae Radix ◽  
Microsomal Cytochrome P450 ◽  
Pyrene Hydroxylase

The present study has determined the effects of Scutellariae Radix (Huangqin) and Gentianae scabrae Radix (Longdan) on liver microsomal cytochrome P450 (P450)-dependent mono-oxygenases using rats pretreated with crude extracts of medicinal herbs. Scutellariae Radix resulted in a 53% decrease of pentoxyresorufin O-dealkylase activity in liver microsomes. In contrast, Gentianae scabrae Radix caused a 50% increase of benzo(a)pyrene hydroxylase activity. Immunoblotting 1A and 2B proteins, respectively. Scutellariae and Gentianae scabrae Radixes had no effects on microsomal aniline hydroxylase activity and P450 2E1 protein.

In vitro biotransformation of atrazine by rat liver microsomal cytochrome P450 enzymes

1998 ◽  
Vol 116 (3) ◽  
pp. 181-198 ◽  
Author(s):  
Nobumitsu Hanioka ◽  
Hideto Jinno ◽  
Ken Kitazawa ◽  
Toshiko Tanaka-Kagawa ◽  
Tetsuji Nishimura ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Cytochrome P450 Enzymes ◽  
Microsomal Cytochrome ◽  
Microsomal Cytochrome P450

Interactions of Monoamine Oxidase Inhibitors, N-Acetylenic Analogues of Tryptamine, with Rat Liver Microsomal Cytochrome P450

1994 ◽  
Vol 46 (5) ◽  
pp. 360-365
Author(s):  
MASSIMO VALOTI ◽  
MARISA COSTANZO ◽  
GIAN PIETRO SGARAGLI ◽  
VIRGILI PEREZ ◽  
ELDIBERTO FERNANDEZ-ALVAREZ ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Monoamine Oxidase ◽  
Rat Liver ◽  
Monoamine Oxidase Inhibitors ◽  
Microsomal Cytochrome ◽  
Microsomal Cytochrome P450

Reductive activation of 1,1-dichloro-1-fluoroethane (HCFC-141b) by phenobarbital- and pyridine-induced rat liver microsomal cytochrome P450

Xenobiotica ◽  
1996 ◽  
Vol 26 (4) ◽  
pp. 425-435 ◽  
Author(s):  
R. Tolando ◽  
R. Ferrara ◽  
N. I. Eldirdiri ◽  
A. Albores ◽  
L. J. King ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Reductive Activation ◽  
Microsomal Cytochrome ◽  
Microsomal Cytochrome P450

scholarly journals IRREVERSIBLE INHIBITION OF RAT LIVER MICROSOMAL CYTOCHROME P450 BY NITRIC OXIDE

1997 ◽  
Vol 75 ◽  
pp. 14
Author(s):  
Y. Minamiyama ◽  
S. Takemura ◽  
Y. Tammoto ◽  
S. Imaoka ◽  
Y. Funae ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cytochrome P450 ◽  
Rat Liver ◽  
Microsomal Cytochrome ◽  
Irreversible Inhibition ◽  
Microsomal Cytochrome P450

scholarly journals In vivo study of the effect of antiviral acyclic nucleotide phosphonate (R)-9-[2- (phosphonomethoxy) propyl]adenine (PMPA, tenofovir) and its prodrug tenofovir disoproxil fumarate on rat microsomal cytochrome P450

2008 ◽  
pp. 761-768
Author(s):  
E Anzenbacherová ◽  
P Anzenbacher ◽  
Z Zídek ◽  
E Buchar ◽  
E Kmoníčková ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Relative Proportion ◽  
Antiviral Agent ◽  
Total Content ◽  
Repeated Administration ◽  
Microsomal Protein ◽  
Microsomal Cytochrome ◽  
Microsomal Cytochrome P450

The total content of rat liver microsomal cytochrome P450 (CYP) significantly decreased after repeated i.p. administration of the antiviral agent tenofovir ((R)-9-[2-(phosphonomethoxy)propyl] adenine) and tenofovir disoproxil at a daily dose 25 mg/kg, although the content of liver microsomal protein did not change. The decrease of the CYP content was accompanied by concomitant increase of the amount of inactive CYP form, cytochrome P420. This effect was confirmed by a parallel study of the activities of selected CYP forms, CYP2E1 (p-nitrophenol hydroxylation) and CYP1A2 (7-ethoxyresorufin deethylation). The activity (expressed relatively to the protein content) of both CYP forms decreased significantly following the decrease of the total CYP. On the other hand, the CYP2E1 activity expressed relatively to the decreasing total CYP content remained unchanged. However, CYP1A2 activity also decreased when calculated relatively to the total native CYP content indicating lower stability of this form. Semiquantitative RT-PCR showed no significant changes in expression of major rat liver microsomal CYP forms after tenofovir treatment. In conclusion, repeated administration of tenofovir in higher doses led to significant decrease of the relative proportion of active liver microsomal CYPs accompanied by a conversion of these enzymes to the inactive form (CYP420) maintaining the sum of CYP proteins unchanged.

N-Dealkylation of Chlorimipramine and Chlorpromazine by Rat Liver Microsomal Cytochrome P450 Isoenzymes

1998 ◽  
Vol 50 (9) ◽  
pp. 1005-1011 ◽  
Author(s):  
Massimo Valoti ◽  
Maria Frosini ◽  
Mitri Palmi ◽  
Francesco Matteis ◽  
Gianpietro Sgaragli
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Microsomal Cytochrome ◽  
Microsomal Cytochrome P450
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