Effect of experimental hyperinsulinemia on insulin binding and glucose transport in isolated rat adipocytes.

1978 ◽  
Vol 235 (1) ◽  
pp. E53 ◽  
Author(s):  
M Kobayashi ◽  
J M Olefsky
Keyword(s):  
Glucose Transport ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Isolated Rat

Effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes

1982 ◽  
Vol 4 (4) ◽  
pp. 261-271 ◽  
Author(s):  
Tj. Wieringa ◽  
G. Bruin ◽  
W. P. M. Meerwijk ◽  
H. M. J. Krans
Keyword(s):  
Glucose Transport ◽  
Insulin Action ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Isolated Rat

Effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes

1982 ◽  
Vol 14 (4) ◽  
pp. 261-271
Author(s):  
Tj. Wieringa ◽  
G. de Bruin ◽  
W. P. M. van Meerwijk ◽  
H. M. J. Krans
Keyword(s):  
Glucose Transport ◽  
Insulin Action ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Isolated Rat

scholarly journals Catecholamine-induced insulin resistance of glucose transport in isolated rat adipocytes

1983 ◽  
Vol 216 (3) ◽  
pp. 737-745 ◽  
Author(s):  
D M Kirsch ◽  
M Baumgarten ◽  
T Deufel ◽  
F Rinninger ◽  
W Kemmler ◽  
...  
Keyword(s):  
Glucose Transport ◽  
Insulin Binding ◽  
Affinity Binding ◽  
Insulin Stimulation ◽  
High Affinity Binding ◽  
Rat Adipocytes ◽  
High Affinity ◽  
Atp Levels ◽  
Isolated Rat ◽  
Stimulation Of

The effects of pre-incubation with isoprenaline and noradrenaline on insulin binding and insulin stimulation of D-glucose transport in isolated rat adipocytes are reported. (1) Pre-incubation of the cells with isoprenaline (0.1-10 microM) in Krebs-Ringer-Hepes [4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid] buffer (30 min, 37 degrees C) at D-glucose concentrations of 16 mM, in which normal ATP levels were maintained, caused a rightward-shift in sensitivity of D-glucose transport to insulin stimulation by 50% and a decrease in maximal responsiveness by 30% (2) [A14-125I]insulin binding was reduced significantly by 35% at insulin concentrations less than 100 mu-units/ml and Scatchard analysis showed that this consisted mainly of a decrease in high-affinity binding. (3) Pre-incubation with catecholamines under the same conditions but at low glucose concentrations (0-5 mM) caused a fall in intracellular ATP levels of 65 and 45% respectively. (4) The fall in ATP additionally lowered insulin binding by 50% at all insulin concentrations and a parallel shift of the binding curves in the Scatchard plot showed that this was due to a decrease in the number of receptors. (5) At low and high ATP concentrations the insulin stimulation of D-glucose transport was inhibited to a similar extent. (6) Pre-incubation with catecholamines thus inhibited insulin stimulation of D-glucose transport in rat adipocytes mainly by a decrease in high-affinity binding of insulin, which was not mediated by low ATP levels. This mechanism may play a role in the pathogenesis of catecholamine-induced insulin resistance in vivo.

scholarly journals In vivo and in vitro effect of p-chlorophenoxyisobutyrate on insulin binding and glucose transport in isolated rat adipocytes.

1985 ◽  
Vol 32 (6) ◽  
pp. 829-836
Author(s):  
NOBUAKI WATANABE ◽  
MASASHI KOBAYASHI ◽  
HIROSHI MAEGAWA ◽  
OSAMU ISHIBASHI ◽  
YASUMITSU TAKATA ◽  
...  
Keyword(s):  
Glucose Transport ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Vitro Effect ◽  
Isolated Rat
Sign in / Sign up

Export Citation Format

Share Document