scholarly journals Longitudinal Raman Spectroscopic Observation of Skin Biochemical Changes due to Chemotherapeutic Treatment for Breast Cancer in Small Animal Model

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Myeongsu Seong ◽  
NoSoung Myoung ◽  
Songhyun Lee ◽  
Hyeryun Jeong ◽  
Sang-Youp Yim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Animal Model ◽  
Raman Spectra ◽  
Fiber Optic ◽  
Animal Experiments ◽  
Small Animal ◽  
Fiber Optic Probe ◽  
Small Animal Model ◽  
Biochemical Compositions ◽  
Optic Probe

The cancer field effect (CFE) has been highlighted as one of indirect indications for tissue variations that are insensitive to conventional diagnostic techniques. In this research, we had a hypothesis that chemotherapy for breast cancer would affect skin biochemical compositions that would be reflected by Raman spectral changes. We used a fiber-optic probe-based Raman spectroscopy to perform preliminary animal experiments to validate the hypothesis. Firstly, we verified the probing depth of the fiber-optic probe (~800 μm) using a simple intravenous fat emulsion-filled phantom having a silicon wafer at the bottom inside a cuvette. Then, we obtained Raman spectra during breast cancer treatment by chemotherapy from a small animal model in longitudinal manner. Our results showed that the treatment causes variations of biochemical compositions in the skin. For further validation, the Raman spectra will have to be collected from more populations and spectra will need to be compared with immunohistochemistry of the breast tissue.

Novel Probe for Laser-Induced Breakdown Spectroscopy and Raman Measurements Using an Imaging Optical Fiber

1998 ◽  
Vol 52 (9) ◽  
pp. 1148-1153 ◽  
Author(s):  
Brian J. Marquardt ◽  
Dimitra N. Stratis ◽  
David A. Cremers ◽  
S. Michael Angel
Keyword(s):  
Raman Spectra ◽  
Optical Fibers ◽  
Fiber Optic ◽  
Laser Induced Breakdown Spectroscopy ◽  
Fiber Optic Probe ◽  
Granite Sample ◽  
Soil Substrate ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
Optic Probe

A fiber-optic probe designed for remote laser-induced breakdown spectroscopy (LIBS), Raman spectroscopy, and Raman imaging has been developed for the microanalysis of solid samples. The probe incorporates both single-strand optical fibers and an image guide and allows atomic emission and Raman analysis of any spot on a solid sample within a 5 mm diameter field of view. The real-time sample imaging aspects of the probe are demonstrated by measuring LIBS spectra from different regions of a granite sample and by measuring the Raman spectra of individual TiO2 and Sr(NO3)2 particles on a soil substrate. The ability to obtain remote Raman images of the TiO2 and Sr(NO3)2 particles on the soil substrate is also demonstrated. In this paper we discuss the design and implementation of the fiber-optic probe for obtaining LIBS spectra, Raman spectra, and Raman images.

Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue In Vivo

1998 ◽  
Vol 68 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Anita Mahadevan-Jansen ◽  
Michele Follen Mitchell ◽  
Nirmala Ramanujam ◽  
Urs Utzinger ◽  
Rebecca Richards-Kortum
Keyword(s):  
Raman Spectra ◽  
Fiber Optic ◽  
Cervical Tissue ◽  
Fiber Optic Probe ◽  
Optic Probe

Use of a multiseparation fiber optic probe for the optical diagnosis of breast cancer

2005 ◽  
Vol 10 (2) ◽  
pp. 024032 ◽  
Author(s):  
Changfang Zhu ◽  
Gregory M. Palmer ◽  
Tara M. Breslin ◽  
Fushen Xu ◽  
Nirmala Ramanujam
Keyword(s):  
Breast Cancer ◽  
Fiber Optic ◽  
Fiber Optic Probe ◽  
Optical Diagnosis ◽  
Optic Probe

Synthesis, performance and growth mechanism of silver nanoparticle coated SERS fiber probe

2019 ◽  
Vol 107 (3) ◽  
pp. 305
Author(s):  
Mengmei Geng ◽  
Yuting Long ◽  
Tongqing Liu ◽  
Zijuan Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Growth Mechanism ◽  
Fiber Optic ◽  
Fiber Optic Probe ◽  
Visible Absorption ◽  
Fiber Probe ◽  
Surface Enhanced Raman ◽  
Optic Probe

Surface-enhanced Raman Scattering (SERS) fiber probe provides abundant interaction area between light and materials, permits detection within limited space and is especially useful for remote or in situ detection. A silver decorated SERS fiber optic probe was prepared by hydrothermal method. This method manages to accomplish the growth of silver nanoparticles and its adherence on fiber optic tip within one step, simplifying the synthetic procedure. The effects of reaction time on phase composition, surface plasmon resonance property and morphology were investigated by X-ray diffraction analysis (XRD), ultraviolet-visible absorption spectrum (UV-VIS absorption spectrum) and scanning electron microscope (SEM). The results showed that when reaction time is prolonged from 4–8 hours at 180 °C, crystals size and size distribution of silver nanoparticles increase. Furthermore, the morphology, crystal size and distribution density of silver nanoparticles evolve along with reaction time. A growth mechanism based on two factors, equilibrium between nucleation and growth, and the existence of PVP, is hypothesized. The SERS fiber probe can detect rhodamin 6G (R6G) at the concentration of 10−6 M. This SERS fiber probe exhibits promising potential in organic dye and pesticide residue detection.

scholarly journals Rat model of smoke inhalation-induced acute lung injury

2021 ◽  
Vol 8 (1) ◽  
pp. e000879
Author(s):  
Premila Devi Leiphrakpam ◽  
Hannah R Weber ◽  
Tobi Ogun ◽  
Keely L Buesing
Keyword(s):  
Animal Model ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Caspase 3 ◽  
Small Animal ◽  
Therapeutic Options ◽  
Smoke Inhalation ◽  
Small Animal Model ◽  
Injury Score ◽  
Confounding Variables

BackgroundAcute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a lethal disease with limited therapeutic options and an unacceptably high mortality rate. Understanding the complex pathophysiological processes involved in the development of ALI/ARDS is critical for developing novel therapeutic strategies. Smoke inhalation (SI) injury is the leading cause of morbidity and mortality in patients with burn-associated ALI/ARDS; however, to our knowledge few reliable, reproducible models are available for pure SI animal model to investigate therapeutic options for ALI/ARDS without the confounding variables introduced by cutaneous burn or other pathology.ObjectiveTo develop a small animal model of pure SI-induced ALI and to use this model for eventual testing of novel therapeutics for ALI.MethodsRats were exposed to smoke using a custom-made smoke generator. Peripheral oxygen saturation (SpO2), heart rate, arterial blood gas, and chest X-ray (CXR) were measured before and after SI. Wet/dry weight (W/D) ratio, lung injury score and immunohistochemical staining of cleaved caspase 3 were performed on harvested lung tissues of healthy and SI animals.ResultsThe current study demonstrates the induction of ALI in rats after SI as reflected by a significant, sustained decrease in SpO2 and the development of diffuse bilateral pulmonary infiltrates on CXR. Lung tissue of animals exposed to SI showed increased inflammation, oedema and apoptosis as reflected by the increase in W/D ratio, injury score and cleaved caspase 3 level of the harvested tissues compared with healthy animals.ConclusionWe have successfully developed a small animal model of pure SI-induced ALI. This model is offered to the scientific community as a reliable model of isolated pulmonary SI-induced injury without the confounding variables of cutaneous injury or other systemic pathology to be used for study of novel therapeutics or other investigation.

Sign in / Sign up

Export Citation Format

Share Document