Involvement of Uronic Acid in Calcium-Induced Aggregation of Deaggregated Water-Insoluble Protein of Human Lens

2015 ◽  
pp. 251-254 ◽  
Author(s):  
K. Satoh ◽  
R. Morishita
Keyword(s):  
Uronic Acid ◽  
Human Lens ◽  
Insoluble Protein ◽  
Induced Aggregation

Calcium-induced aggregation of the urea-deaggregated human lens insoluble protein

1990 ◽  
Vol 50 (6) ◽  
pp. 719-723 ◽  
Author(s):  
Kenshi Satoh ◽  
Hiroshi Adachi ◽  
Shumpo Yamashita ◽  
Hiroyuki Hirano ◽  
Parida Yasin ◽  
...  
Keyword(s):  
Human Lens ◽  
Insoluble Protein ◽  
Induced Aggregation

Characterization of the insoluble protein fraction in the ocular lens

1969 ◽  
Vol 47 (12) ◽  
pp. 1115-1119 ◽  
Author(s):  
Sidney Lerman
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Protein Fraction ◽  
Human Lens ◽  
Ocular Lens ◽  
Insoluble Protein ◽  
Alpha Crystallin ◽  
Gamma Crystallin ◽  
Insoluble Protein Fraction

The insoluble protein fraction of the ocular lens has been studied in four different species: the rat, dogfish, bovine, and human. These studies were performed in order to characterize the composition and formation of this protein in the ocular lens. On the basis of amino acid analyses, spectrophotometric determinations, thin-layer electrophoresis on Pevikon plates, and immunochemical data, it was concluded that in the rat and dogfish lens, the insoluble protein fraction derives mainly from the soluble gamma crystallin fraction. However, in the bovine and human lens, the insoluble protein fraction appears to derive mainly from the soluble alpha crystallin fraction.

scholarly journals Mercury-induced aggregation of human lens γ-crystallins reveals a potential role in cataract disease

2018 ◽  
Vol 23 (7) ◽  
pp. 1105-1118 ◽  
Author(s):  
J. A. Domínguez-Calva ◽  
M. L. Pérez-Vázquez ◽  
E. Serebryany ◽  
J. A. King ◽  
L. Quintanar
Keyword(s):  
Potential Role ◽  
Human Lens ◽  
Induced Aggregation

Effect of aging on the water-soluble and water-insoluble protein pattern in normal human lens

1982 ◽  
Vol 34 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Peter J. Ringens ◽  
Herman J. Hoenders ◽  
Hans Bloemendal
Keyword(s):  
Protein Pattern ◽  
Water Soluble ◽  
Human Lens ◽  
Insoluble Protein ◽  
Normal Human

Ultrastructural Characteristics of Platelets Separated from Plasma by ADP-Induced Aggregation

1976 ◽  
Vol 34 ◽  
pp. 164-165
Author(s):  
B.A. Shinoda ◽  
M.D. Hardison ◽  
S.F. Mohammad ◽  
H.Y.K. Chuang ◽  
R.G. Mason
Keyword(s):  
Blood Platelets ◽  
Gel Filtration ◽  
Differential Centrifugation ◽  
Ultrastructural Characteristics ◽  
Induced Aggregation

The utilization of blood platelets in experimentation frequently requires their separation from blood and subsequent resuspension in media of known composition. Several methods are available for preparation of isolated platelets (1-3) by differential centrifugation or gel filtration, but most methods are tedious and time consuming. Often platelets obtained by use of such methods are in a state different functionally and ultrastructurally from that of platelets in plasma (4).Recently Mohammad, Reddick, and Mason (5) reported a method in which platelets were separated from plasma by ADP-induced aggregation, washed several times, and then incubated in a carefully selected medium that resulted in deaggregation of platelets.

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