Validated UPLC/QTOF-MS Method for Quantitative Determination of Salannin in Wistar Rat Plasma: In Vitro Assay, Ex Vivo Stability Effects and Pharmacokinetic Investigation

2012 ◽  
Vol 16 (1) ◽  
pp. 173-178
Author(s):  
Md Tanwir Athar ◽  
S. Mohamed Musthaba ◽  
Javed Ali ◽  
Sanjula Baboota ◽  
Sayeed Ahmad
Keyword(s):  
Quantitative Determination ◽  
Ex Vivo ◽  
Wistar Rat ◽  
Rat Plasma ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Pharmacokinetic Investigation

Applicability of the OECD 455 in-vitro assay for determination of hERa agonistic activity of isoflavonoids

2020 ◽  
Vol 386 ◽  
pp. 114831 ◽  
Author(s):  
Darja Gramec Skledar ◽  
Václav Tvrdý ◽  
Maša Kenda ◽  
Anamarija Zega ◽  
Milan Pour ◽  
...  
Keyword(s):  
In Vitro Assay ◽  
Vitro Assay ◽  
Agonistic Activity

scholarly journals Interleukin-6 Promotes Anti-OspA Borreliacidal Antibody Production In Vitro

2006 ◽  
Vol 13 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Erik L. Munson ◽  
Dean T. Nardelli ◽  
K. H. Kevin Luk ◽  
Monica C. Remington ◽  
Steven M. Callister ◽  
...  
Keyword(s):  
Lymph Node ◽  
B Lymphocytes ◽  
Antibody Production ◽  
Interleukin 6 ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Lymph Node Cells ◽  
In Vitro Treatment

ABSTRACT Determination of the immunological mediators responsible for promoting the production of borreliacidal antibody may facilitate the development of an improved borreliosis vaccine for human and veterinary use. Previously, we developed an in vitro assay to determine if borreliacidal antibody production could be augmented by treatment with different cytokines. In this study, in vitro treatment of lymph node cells producing borreliacidal antibody with recombinant interleukin-6 (rIL-6) resulted in a fourfold enhancement of anti-OspA borreliacidal antibody. Moreover, rIL-6 enhanced Western immunoblot titers and increased the number of B lymphocytes. In contrast, treatment of anti-OspA borreliacidal antibody-producing cells with anti-IL-6 resulted in a fourfold reduction in borreliacidal activity. Treatment with anti-IL-6 also inhibited enhanced borreliacidal antibody production induced by anti-gamma interferon. These data suggest that IL-6 plays a significant role in the production of anti-OspA borreliacidal antibodies.

Spectrophotometric determination of de novo hemozoin/β-hematin formation in an in vitro assay

2004 ◽  
Vol 325 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Abhai K Tripathi ◽  
Shabana I Khan ◽  
Larry A Walker ◽  
Babu L Tekwani
Keyword(s):  
Spectrophotometric Determination ◽  
De Novo ◽  
In Vitro Assay ◽  
Vitro Assay

Use of an in Vitro Assay for Determination of Biofilm-Forming Capacity of Bacteria in Chronic Rhinosinusitis

2006 ◽  
Vol 20 (5) ◽  
pp. 434-438 ◽  
Author(s):  
Zohra Bendouah ◽  
Jean Barbeau ◽  
Walid Abou Hamad ◽  
Martin Desrosiers
Keyword(s):  
Chronic Rhinosinusitis ◽  
In Vitro Assay ◽  
Vitro Assay

THE BIOASSAY OF LONG-ACTING THYROID STIMULATOR: A COMPARISON OF TWO METHODS

1971 ◽  
Vol 49 (3) ◽  
pp. 487-492 ◽  
Author(s):  
JANICE M. ENSOR ◽  
PAT KENDALL-TAYLOR ◽  
D. S. MUNRO ◽  
B. R. SMITH
Keyword(s):  
Thyroid Stimulating Hormone ◽  
In Vitro Assay ◽  
Assay Method ◽  
Vitro Assay ◽  
In Vitro Method ◽  
Long Acting ◽  
Stimulating Hormone

SUMMARY The McKenzie (1958) assay method and an in-vitro assay method (Brown & Munro, 1967) for the determination of long-acting thyroid stimulator have been compared. Differences in the results were not great: the in-vitro method is simpler to perform and more precise. The sensitivity of the two methods is similar. The in-vitro method, when used with antiserum to thyroid-stimulating hormone in the medium, provided a satisfactory and simple means for the assay of long-acting thyroid stimulator in the serum of thyrotoxic patients.

Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (Sf9) insect cells by a selective 1H/15N NMR in vitro assay

2000 ◽  
Vol 79 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Magnus Doverskog ◽  
Ulla Jacobsson ◽  
Bodgan E Chapman ◽  
Philip W Kuchel ◽  
Lena Häggström
Keyword(s):  
Spodoptera Frugiperda ◽  
Insect Cells ◽  
Glutamate Synthase ◽  
In Vitro Assay ◽  
15N Nmr ◽  
Vitro Assay ◽  
Sf9 Insect Cells
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