Prolactin Receptor in Human Mammary Carcinoma

1979 ◽  
Vol 65 (6) ◽  
pp. 695-702 ◽  
Author(s):  
Raffaele Di Carlo ◽  
Giampiero Muccioli
Keyword(s):  
Protein Concentration ◽  
Binding Capacity ◽  
Specific Binding ◽  
Prolactin Receptor ◽  
Scatchard Analysis ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Human Mammary Carcinoma ◽  
Human Breast Carcinomas ◽  
Lactogenic Hormones

The specific binding of labelled human prolactin was determined in 83 human breast carcinomas. Twenty-seven tumors (32.5 %) contained specific binding for prolactin of at least 1 % and were considered prolactin receptor positive. The binding was found linearly related to membrane protein concentration and specific only for lactogenic hormones. By Scatchard analysis the dissociation constant appeared similar to that observed in other target tissues, with a low binding capacity.

Three radioligands compared for determining cytoplasmic estrogen-receptor content of human breast carcinomas.

1982 ◽  
Vol 28 (3) ◽  
pp. 532-537 ◽  
Author(s):  
P C Pieslor ◽  
R E Gibson ◽  
W C Eckelman ◽  
K K Oates ◽  
B Cook ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Specific Activity ◽  
Scatchard Analysis ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Human Breast Carcinomas ◽  
Receptor Assay ◽  
Receptor Concentration ◽  
To Come

Abstract We compared three radioligands for use in a cytoplasmic estrogen-receptor assay, using pooled cytosol from human breast adenocarcinomas. The estrogen receptor content was determined in vitro by a dextran-coated charcoal method involving a 4-h incubation with and without diethylstilbestrol. Tritiated moxestrol failed to come to equilibrium in 4 h, thereby preventing the use of conventional one-component Scatchard analysis. The use of 125I-labeled estradiol resulted in a higher estimate of estrogen-receptor concentration than that obtained with use of tritiated estradiol. This overestimation was not corrected by Scatchard, double-reciprocal, or Woolf plots, or by two different methods of data analysis: least squared and "robust." An underestimation of the specific activity of iodoestradiol with respect to that of tritiated estradiol and an unrecognized second component of nonreceptor binding could explain this disparity.

scholarly journals BUB1infrequently mutated in human breast carcinomas

2003 ◽  
Vol 22 (5) ◽  
pp. 420-420 ◽  
Author(s):  
Anita Langerød ◽  
Maria Strømberg ◽  
Koei Chin ◽  
Vessela N. Kristensen ◽  
Anne-Lise Børresen-Dale
Keyword(s):  
Breast Carcinomas ◽  
Human Breast ◽  
Human Breast Carcinomas

scholarly journals Fgf10 is an oncogene activated by MMTV insertional mutagenesis in mouse mammary tumors and overexpressed in a subset of human breast carcinomas

Oncogene ◽  
2004 ◽  
Vol 23 (36) ◽  
pp. 6047-6055 ◽  
Author(s):  
Vassiliki Theodorou ◽  
Mandy Boer ◽  
Britta Weigelt ◽  
Jos Jonkers ◽  
Martin van der Valk ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Mammary Tumors ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Human Breast Carcinomas ◽  
Mouse Mammary Tumors
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