scholarly journals Occult hepatitis C virus infection in patients with malignant lymphoproliferative disorders

2020 ◽  
Vol 34 ◽  
pp. 205873842096120
Author(s):  
Abeya A Lotfi ◽  
Asmaa E Mohamed ◽  
Nahela A Shalaby ◽  
Deena S Eissa ◽  
Ehab El-Dabaa ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Treatment Protocol ◽  
Lymphoproliferative Disorders ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Hepatitis C Virus Infection ◽  
Occult Hepatitis ◽  
Occult Hepatitis C

Despite the link between HCV and malignant lymphoproliferative disorders has been established, the association between occult hepatitis C virus infection and malignant lymphoproliferative disorders remains obscure. The present study intended to identify the possible association between occult HCV infection and malignant lymphoproliferative disorders. Newly diagnosed patients with LPDs were screened for the presence of HCV-RNA in both plasma and PBMCs. PBMCs of the subjects were also, examined by transmission and immuno-electron microscopy. LPD patients showed a high percentage of HCV infection (71.9%): OCI-HCV (37.5%) and HCV (34.38%). Meanwhile, 28.13% of LPD patients did not show any evidence of HCV infection. Ultrastructural examination of PBMCs revealed the presence of intracytoplasmic vacuoles enclosing viral like particles, which were less prominent in occult HCV patients. The possibility of occult HCV should be considered in patients with LPDs which can be helpful in the management of the treatment protocol in order to set up a balance between the control of the tumor progression and minimizing post chemotherapy complications related to HCV infection.

Prevalence of occult hepatitis C virus infection in Iranian patients with lymphoproliferative disorders

2012 ◽  
Vol 85 (2) ◽  
pp. 235-240 ◽  
Author(s):  
Maryam Farahani ◽  
Farah Bokharaei-Salim ◽  
Masood Ghane ◽  
Ali Basi ◽  
Parisa Meysami ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Lymphoproliferative Disorders ◽  
Hepatitis C Virus Infection ◽  
Occult Hepatitis ◽  
Occult Hepatitis C ◽  
Iranian Patients

scholarly journals Prevalence of Occult Hepatitis C Virus Infection in Beta-Thalassemia Major Patients in Ahvaz, Iran

2021 ◽  
Author(s):  
sepideh Nasimzadeh ◽  
azarakhsh azaran ◽  
shahram Jalilian ◽  
Manoochehr Makvandi ◽  
Seyed Saeid Seyedian ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Thalassemia Major ◽  
Beta Thalassemia ◽  
Hcv Rna ◽  
Hepatitis C Virus Infection ◽  
Occult Hepatitis ◽  
Beta Thalassemia Major ◽  
Occult Hepatitis C

Abstract Background: Occult hepatitis C virus infection (OCI) is defined by the presence of HCV RNA in peripheral blood mononuclear cells (PBMCs) and liver tissue cells despite the absence of HCV RNA in plasma. Currently, OCI is classified into two types: seropositive OCI (anti-HCV positive and serum HCV-RNA negative) and seronegative OCI (anti-HCV and serum HCV-RNA negative). Beta-Thalassemia is described as a blood disorder, which decreases the synthesis of hemoglobin. Repeated blood transfusion is the standard treatment for patients with beta-thalassemia major (BTM) that increases the risk of exposure to infectious agents. This study aimed to investigate the prevalence of OCI among BTM patients.Materials and Methods: plasma and PBMCs were collected from 90 BTM patients and screened for HCV antibody using the ELISA kit commercially as the first step. Then nested-RT PCR was performed on extractions of plasma and PBMC. Positive samples of HCV RNA from PBMCs were sequenced and aligned to construct the HCV phylogenetic tree to assess the homology of sequences compared to the reference sequences retrieved from GenBank.Results: Seventy-nine out of 90 cases (87.8%) indicated negative results for HCV Ab (seronegative), while 11 patients (12.2%) were seropositive. HCV RNA was found in PBMCs samples of four patients (66.66%) with negative HCV Ab (seronegative) and two patients (33.3%) with positive HCV Ab (seropositive). HCV RNA was not detected in plasma samples of these six patients. Overall six out of 90 patients (6.7%) had OCI. HCV genotyping revealed that all six patients infected with HCV subtype 3a.Conclusion: We indicated the high frequency of OCI in BTM patients. Nevertheless, more attention is warranted, considering the importance of this infection. Also, further studies are necessary to determine the actual prevalence of OCI among BTM patients in Iran.

Prevalence of occult hepatitis C virus infection in Iranian patients with beta thalassemia major

2016 ◽  
Vol 161 (7) ◽  
pp. 1899-1906 ◽  
Author(s):  
Mohammad-Navid Bastani ◽  
Farah Bokharaei-Salim ◽  
Hossein Keyvani ◽  
Maryam Esghaei ◽  
Seyed Hamidreza Monavari ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Thalassemia Major ◽  
Beta Thalassemia ◽  
Hepatitis C Virus Infection ◽  
Occult Hepatitis ◽  
Beta Thalassemia Major ◽  
Occult Hepatitis C ◽  
Iranian Patients

Prevalence and Risk Factors of Occult Hepatitis C Virus infection in one Tertiary Egyptian centre

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Maha Habeeb ◽  
Abd-Elmohsen Eldesoky ◽  
Maysaa Zaki ◽  
Mona Arafa ◽  
Dina Elhammady ◽  
...  
Keyword(s):  
Risk Factors ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Hepatitis C Virus Infection ◽  
Occult Hepatitis ◽  
Occult Hepatitis C

scholarly journals Characterization of Occult Hepatitis C Virus (Hcv) Infection among Hemodialysis Patient

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Siti Nurul Fazlin Abdul Rahman ◽  
Hairul Aini binti Hamzah ◽  
Mohammed Imad Mustafa ◽  
Mohamed Hadzri Hasmoni
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hemodialysis Patients ◽  
Hcv Infection ◽  
Viral Rna ◽  
Cell Counting ◽  
Hcv Rna ◽  
Viral Rnas ◽  
Occult Hepatitis ◽  
Occult Hepatitis C

Introduction: The existence of new entity called occult hepatitis C virus (HCV) has become a raising and escalating concern among healthcare professionals worldwide. It is defined by the presence of viral RNA in liver and/or peripheral blood mononuclear cells (PBMCs) within non HCV-infected patients. Previous study had shown the occult HCV is infectious and capable of transmitting the virus to another host. Till today, HCV infection remains common among hemodialysis patients despite having the best preventive plans. Because of this, there is a significant concern about the source of viral transmission. The aim of the study was to identify and characterize occult HCV infection in PBMC sample of hemodialysis patients. This was an observational and cross sectional study. Materials and method: PBMCs were isolated from the whole blood using Ficoll-gradient centrifugation technique. The PBMCs were then subjected for cell counting and stored in -70O C until further used. HCV RNA were extracted from these cells and viral RNA were subjected for molecular assays, immune cells analysis and cells culture. Results: PBMCs were isolated from eleven (11) study patients and five (5) anti-HCV positive (control) patients. By using automated flow cytometry, PBMCs of each sample were counted and the average number of cells obtained range from 2x104 to 5x106 cells/ ml. Viral RNAs were extracted and quantitatively measured by using NanoDrop Spectrophotometers. The viral RNAs concentration obtained were between 24.7 and 258.9 ng/ml. The RNAs would be subjected for purification (ethanol precipitation) and further assays. Conclusion: The final findings might contribute to the clinical management of dialysis patients.

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