scholarly journals Purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus Rhizopus arrhizus

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Manoj Saxena ◽  
Rohit Kumar Sharma ◽  
Josell Ramirez-Paz ◽  
Arthur D. Tinoco ◽  
Kai Griebenow
1997 ◽  
Vol 52 (11-12) ◽  
pp. 740-746 ◽  
Author(s):  
Röbbe Wünschiers ◽  
Thomas Zinn ◽  
Dietmar Linder ◽  
Rüdiger Schulz

Abstract Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monoraphidium braunii. The ferrocyto-chrome shows typical UV/VIS absorption peaks at 552.9, 521.9 and 415.7 nm. The apparent molecular mass was estimated to be 12 kD a by SDS-PAGE. EPR-spectroscopy at 20K shows resonances indicative for two distinct low-spin heme forms.


1981 ◽  
Vol 10 (3) ◽  
pp. 267-270 ◽  
Author(s):  
Kazunobu Matsushita ◽  
Kenji Tayama ◽  
Emiko Shinagawa ◽  
Osao Adachi ◽  
Minoru Ameyama

2009 ◽  
Vol 102 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Patrick D. Bell ◽  
Yueyong Xin ◽  
Robert E. Blankenship

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