scholarly journals Peanut components measured by ISAC: comparison with ImmunoCap and clinical relevance in peanut allergic children

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
H. K. Brand ◽  
M. W. J. Schreurs ◽  
J. A. M. Emons ◽  
R. Gerth van Wijk ◽  
H. de Groot ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Solid Phase ◽  
Area Under The Curve ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Golden Standard ◽  
Ara H 6 ◽  
Food Challenges ◽  
Double Blinded

Abstract Background Specific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy. Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard. Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0.906 and 0.902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r = 0.88; p < 0.01). Conclusion Ara h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy in Dutch children, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of different peanut components using ISAC is an advantage and clinically useful to detect peanut allergic children that are Ara h 2 negative but sensitized to other peanut proteins such as Ara h 6.

scholarly journals Peanut Components Measured by ISAC: Comparison With ImmunoCap and Clinical Relevance in Peanut Allergic Children

2021 ◽  
Author(s):  
Kim Brand ◽  
Marco Schreurs ◽  
Joyce Emons ◽  
Roy Gerth van Wijk ◽  
Hans de Groot ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Solid Phase ◽  
Area Under The Curve ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Golden Standard ◽  
Ara H 6 ◽  
Food Challenges ◽  
Double Blinded

Abstract BackgroundSpecific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy.Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard.Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0,906 and 0,902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r=0.88. p<0.01). ConclusionAra h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of Ara h 6 and Ara h 2 sIgE using ISAC is an advantage and clinically useful to detect peanut allergic children that are monosensitized to Ara h 6.

scholarly journals Diagnostic Value of Specific IgE to Peanut and Ara h 2 in Korean Children with Peanut Allergy

2016 ◽  
Vol 8 (2) ◽  
pp. 156 ◽  
Author(s):  
Hye-Young Kim ◽  
Youngshin Han ◽  
Kwanghoon Kim ◽  
Ji Young Lee ◽  
Min-Ji Kim ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Korean Children ◽  
Ara H 2

The diagnostic value of specific IgE to Ara h 2 to predict peanut allergy in children is comparable to a validated and updated diagnostic prediction model

2013 ◽  
Vol 131 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Rob J.B. Klemans ◽  
Dianne Otte ◽  
Mirjam Knol ◽  
Edward F. Knol ◽  
Yolanda Meijer ◽  
...  
Keyword(s):  
Prediction Model ◽  
Peanut Allergy ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2

scholarly journals Impact of Cross-reactivity between Major Peanut Allergens Ara h 2 and Ara h 6 on Specific IgE Measurements

2018 ◽  
Vol 141 (2) ◽  
pp. AB237
Author(s):  
Blanche Guillon ◽  
Hervé Bernard ◽  
Evelyne Paty ◽  
Stephen C. Dreskin ◽  
Karine Adel-Patient ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Ara H 2 ◽  
Peanut Allergens ◽  
Ara H 6

Utility of Gal d 1-Specific IgE Levels in Predicting Reactivity to Boiled Egg in Chinese Children

2021 ◽  
pp. 1-7
Author(s):  
Dan Ming ◽  
Rongxiu Zheng
Keyword(s):  
Specific Ige ◽  
Diagnostic Value ◽  
Egg White ◽  
Chinese Children ◽  
Egg Allergy ◽  
Clinical Sensitivity ◽  
Receiver Operating Characteristic Curves ◽  
Allergen Components ◽  
Quantitative Measurements ◽  
Food Challenges

<b><i>Background:</i></b> Many researchers have reported predicting the outcome of oral food challenges (OFCs) on the basis of specific IgE (sIgE) levels. However, the clinical usefulness of the determination of IgE antibodies to egg allergen components in Chinese children with suspected boiled egg allergy is not well studied. <b><i>Objective:</i></b> Our objective was to assess the diagnostic performance of sIgE to egg white and Gal d 1, 2, 3, and 5 based on the open challenge outcome for boiled egg. <b><i>Methods:</i></b> A total of 48 child patients with a suspect of boiled egg allergy were included. Serum egg white and Gal d 1, 2, 3, and 5 sIgE were measured by ImmunoCAP. Diagnostic value was assessed by area under the receiver operating characteristic curves (AUC). <b><i>Results:</i></b> Using the OFC results as the reference parameter, Gal d 1 sIgE had the highest AUC (0.84) compared with egg white (0.77) and other investigated components (ranging from 0.51 to 0.71). The clinical sensitivity and specificity for the sIgE to Gal d 1 at optimal cutoff (6.15 kU<sub>A</sub>/L) were 73.7% and 96.7%, respectively. Sensitization to Gal d 1 with a cutoff value of &#x3e;7.48 kU<sub>A</sub>/L indicated a 90% probability of positive challenge. <b><i>Conclusion:</i></b> Quantitative measurements of Gal d 1 sIgE antibodies using ImmunoCAP are useful in the management of boiled egg allergy in Chinese children.

Ara h 2 and Ara h 6 sensitization predicts peanut allergy in Mediterranean pediatric patients

2014 ◽  
Vol 25 (7) ◽  
pp. 662-667 ◽  
Author(s):  
Chantal Agabriel ◽  
Ouafeh Ghazouani ◽  
Joëlle Birnbaum ◽  
Valérie Liabeuf ◽  
Françoise Porri ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Pediatric Patients ◽  
Ara H 2 ◽  
Ara H 6

scholarly journals Ara h 6-specific IgE is a good predictor of peanut allergy in Japanese children

2020 ◽  
Vol 13 (8) ◽  
pp. 100421
Author(s):  
Sakura Sato ◽  
Noriyuki Yanagida ◽  
Kazuko Kutsuwada ◽  
Naoko Fusayasu ◽  
Ken-ichi Nagakura ◽  
...  
Keyword(s):  
Good Predictor ◽  
Peanut Allergy ◽  
Specific Ige ◽  
Japanese Children ◽  
Ara H 6

The diagnostic accuracy of specific IgE to Ara h 6 in adults is as good as Ara h 2

Allergy ◽  
2014 ◽  
Vol 69 (8) ◽  
pp. 1112-1114 ◽  
Author(s):  
R. J. B. Klemans ◽  
E. F. Knol ◽  
C. A. F. M. Bruijnzeel-Koomen ◽  
A. C. Knulst
Keyword(s):  
Diagnostic Accuracy ◽  
Specific Ige ◽  
Ara H 2 ◽  
Ara H 6
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