Utility of Gal d 1-Specific IgE Levels in Predicting Reactivity to Boiled Egg in Chinese Children

2021 ◽  
pp. 1-7
Author(s):  
Dan Ming ◽  
Rongxiu Zheng
Keyword(s):  
Specific Ige ◽  
Diagnostic Value ◽  
Egg White ◽  
Chinese Children ◽  
Egg Allergy ◽  
Clinical Sensitivity ◽  
Receiver Operating Characteristic Curves ◽  
Allergen Components ◽  
Quantitative Measurements ◽  
Food Challenges

<b><i>Background:</i></b> Many researchers have reported predicting the outcome of oral food challenges (OFCs) on the basis of specific IgE (sIgE) levels. However, the clinical usefulness of the determination of IgE antibodies to egg allergen components in Chinese children with suspected boiled egg allergy is not well studied. <b><i>Objective:</i></b> Our objective was to assess the diagnostic performance of sIgE to egg white and Gal d 1, 2, 3, and 5 based on the open challenge outcome for boiled egg. <b><i>Methods:</i></b> A total of 48 child patients with a suspect of boiled egg allergy were included. Serum egg white and Gal d 1, 2, 3, and 5 sIgE were measured by ImmunoCAP. Diagnostic value was assessed by area under the receiver operating characteristic curves (AUC). <b><i>Results:</i></b> Using the OFC results as the reference parameter, Gal d 1 sIgE had the highest AUC (0.84) compared with egg white (0.77) and other investigated components (ranging from 0.51 to 0.71). The clinical sensitivity and specificity for the sIgE to Gal d 1 at optimal cutoff (6.15 kU<sub>A</sub>/L) were 73.7% and 96.7%, respectively. Sensitization to Gal d 1 with a cutoff value of &#x3e;7.48 kU<sub>A</sub>/L indicated a 90% probability of positive challenge. <b><i>Conclusion:</i></b> Quantitative measurements of Gal d 1 sIgE antibodies using ImmunoCAP are useful in the management of boiled egg allergy in Chinese children.

scholarly journals Component resolved diagnosis of egg yolk is an indispensable part of egg allergy

2021 ◽  
Vol 49 (2) ◽  
pp. 6-14
Author(s):  
Huang Lunhui ◽  
Shao Yanhong ◽  
Li Shaoshen ◽  
Bao Huijing ◽  
Liu Yunde ◽  
...  
Keyword(s):  
Operating Characteristic ◽  
Egg Yolk ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Egg White ◽  
Enzyme Linked Immunosorbent Assay ◽  
Egg Allergy ◽  
Component Resolved Diagnosis ◽  
Allergen Components

Introduction and objectives: It was urgent to explain the role of egg yolk allergen sensitization to the egg allergic population and we would evaluate the diagnostic value of allergen components in whole eggs, including egg white and egg yolk.Materials and methods: Firstly, we collected 99 positive and 21 negative sera against egg allergy. Then we used modified enzyme linked immunosorbent assay (ELISA) to survey specific IgE (sIgE) to all-proven and single component in eggs, Ovomucoid (Gal d 1), Ovalbumin (Gal d 2), Ovotransferrin (Gal d 3), Lysozyme C (Gal d 4), Serum Albumin (Gal d 5), and YGP42(Gal d 6) in allergic and non-allergic populations. Last but not least, we studied the sIgE reactivities to egg allergen components by receiver operating characteristic (ROC) analysis.Results: Among egg-allergic individuals, nearly 10% were sensitized to five of six egg allergen components, and the cross-reaction frequency between two egg yolk allergens with Gal d 1 was about 30% in the groups diagnosed with egg allergy or non-allergy. The best component-combination diagnosis in egg allergy of Gal d 1+ Gal d 6 demonstrated the largest area under curve (AUC) of 0.994.Conclusions: Our results suggested that there were individual differences in allergenicity of different egg allergen components, especially in the samples negative to egg allergy diagnosed but sensitive to egg yolk components. It was indicated that component resolved diagnosis of egg yolk improved the value for egg allergy management indispensably.

scholarly journals Unscrambling Egg Allergy: The Diagnostic Value of Specific IgE Concentrations and Skin Prick Tests for Ovomucoid and Egg White in the Management of Children with Hen’s Egg Allergy

ISRN Allergy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
D. E. Marriage ◽  
M. Erlewyn-Lajeunesse ◽  
D. J. Unsworth ◽  
A. J. Henderson
Keyword(s):  
Specific Ige ◽  
Diagnostic Value ◽  
Egg White ◽  
Optimal Decision ◽  
Egg Allergy ◽  
Characteristic Analysis ◽  
Decision Points ◽  
Specific Ige Antibodies ◽  
Skin Prick Tests ◽  
Antibody Levels

Resolution of egg allergy occurs in the majority of egg allergic children. Positive specific IgE antibodies to ovomucoid (OVM) have been suggested to be of greater predictive value for persistent egg allergy than specific IgE to egg white. The performance of OVM-specific IgE antibody levels in a cohort of children referred for a routine egg challenge was compared with egg white specific IgE levels in predicting a positive egg challenge. 24/47 subjects had persistent egg allergy. Receiver operating characteristic analysis showed that OVM-specific IgE testing was the most useful test for the diagnosis of persistent egg allergy. The optimal decision points for the prediction of persistent egg allergy were >0.35 kUA/L for specific IgE levels to both EW and OVM, and ≥3 mm for SPT. Children with specific IgE levels suggestive of persistent egg allergy need not be subject to an egg provocation challenge, reducing both costs and risks to the child.

Egg white specific IgE levels in serum as clinical reactivity predictors in the course of egg allergy follow-up

2009 ◽  
Vol 21 (4p1) ◽  
pp. 634-639 ◽  
Author(s):  
Elena Montesinos ◽  
Antonio Martorell ◽  
Rubén Félix ◽  
Juan Carlos Cerdá
Keyword(s):  
Specific Ige ◽  
Egg White ◽  
Egg Allergy

Predictive power of ovomucoid and egg white specific IgE in heated egg oral food challenges

2018 ◽  
Vol 6 (6) ◽  
pp. 2115-2117.e6 ◽  
Author(s):  
Kyohei Takahashi ◽  
Noriyuki Yanagida ◽  
Sakura Sato ◽  
Motohiro Ebisawa
Keyword(s):  
Predictive Power ◽  
Specific Ige ◽  
Egg White ◽  
Food Challenges

scholarly journals Component-Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Jiayi Zhang ◽  
Yongming Shen ◽  
Junpu Li ◽  
Huiqiang Li ◽  
Ping Si
Keyword(s):  
Local Population ◽  
Clinical History ◽  
Specific Ige ◽  
Egg White ◽  
Diagnostic Study ◽  
Egg Allergy ◽  
Sige Level ◽  
Specific Igg4 ◽  
Specific Igg ◽  
Egg Components

Background. Egg component-specific IgE can be useful to evaluate and diagnose egg allergy, but their prevalence and clinical significance remain unclear in the local population. Previous studies have led to contradictory results regarding the value of specific IgG and specific IgG4 in sensitization. Objective. We aimed to determine the level of specific IgE, IgG, and IgG4 antibodies to the major egg allergens in egg-allergic children. Methods. Children from 6 months to 10 years of age were recruited. Egg allergy was confirmed by either a strong clinical history or an increased egg white-sIgE level. Other allergies were diagnosed by reactivity to other allergens but without egg-related symptoms and history. The serum sIgE, sIgG, and sIgG4 levels to major egg allergenic components (Gal d 1, Gal d 2, Gal d 3, Gal d 4, and Gal d 5), sIgE level to egg white, and tIgE level were determined by light-initiated chemiluminescent assay (LICA), ELISA, or ImmunoCAP. Results. Egg-allergic children had significantly higher levels of sIgE, sIgG, and sIgG4 to egg components than nonallergic children. Gal d 2 was the predominant allergen, and Gal d 2 sIgE level correlated with the egg white-sIgE level. Ratios of sIgE/sIgG4 to egg components were highest before 1 year of age and dropped gradually in the first decade of life. Conclusion. Patterns of sIgE to egg components could distinguish different forms of egg allergy. Ratios of sIgE/sIgG4 could be useful in predicting tolerance in egg-sensitive subjects, but this needs further evaluation and investigation using more accurate models.

scholarly journals Peanut components measured by ISAC: comparison with ImmunoCap and clinical relevance in peanut allergic children

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
H. K. Brand ◽  
M. W. J. Schreurs ◽  
J. A. M. Emons ◽  
R. Gerth van Wijk ◽  
H. de Groot ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Solid Phase ◽  
Area Under The Curve ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Golden Standard ◽  
Ara H 6 ◽  
Food Challenges ◽  
Double Blinded

Abstract Background Specific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy. Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard. Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0.906 and 0.902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r = 0.88; p < 0.01). Conclusion Ara h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy in Dutch children, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of different peanut components using ISAC is an advantage and clinically useful to detect peanut allergic children that are Ara h 2 negative but sensitized to other peanut proteins such as Ara h 6.

scholarly journals Legume and Sesame Oral Food Challenge Outcomes

2020 ◽  
Author(s):  
Jacob J. Pozin ◽  
Ashley L. Devonshire ◽  
Kevin Tom ◽  
Melanie Makhija ◽  
Anne Marie Singh
Keyword(s):  
Logistic Regression ◽  
Regression Models ◽  
Operating Characteristic ◽  
Food Challenge ◽  
Specific Ige ◽  
Oral Food Challenge ◽  
Food Allergens ◽  
Logistic Regression Models ◽  
Receiver Operating Characteristic Curves ◽  
Food Challenges

Abstract Background: Legume and sesame are emerging food allergens. The utility of specific IgE testing (sIgE) to predict clinical reactivity to these allergens is not well described.Objective: To describe clinical outcomes and sIgE in sesame and legume OFCs. Methods: We performed a retrospective review of 74 legume and sesame oral food challenges (OFC) performed between 2007-2017 at the Ann & Robert H. Lurie Children’s Hospital of Chicago. Clinical data, OFC outcome, and sIgE to legume and sesame were collected. Receiver operating characteristic curves (ROC) and logistic regression models predicting OFC outcome were generated. Results: Twenty-eight patients (median age 6.15 years) passed legume OFC (84.85%), and twenty-five patients (median age 5.91 years) passed sesame OFC (60.98%). The median sIgE to legume was 1.41 kUa/L, and 2.34 kUa/L, to sesame. For patients who failed legume OFC, 67% had cutaneous, 16.5% had gastrointestinal, and 16.5% had anaphylaxis. Of these reactions, 80% were controlled with Benadryl alone and 20% required epinephrine. For patients who failed sesame OFC, 53% had cutaneous, 12% had gastrointestinal, and 35% had anaphylaxis. Of these reactions, 6% required epinephrine, 31% were controlled with Benadryl alone, and 63% required additional epinephrine or steroids. Conclusion: Most OFC to legumes were passed and reactions to failed legume OFCs were more likely to be non-severe. Failing an OFC to sesame was almost twice as likely compared to failing a legume OFC, and reactions to failed sesame OFC were often more severe. Sesame sIgE did not correlate with OFC outcome.

Ovomucoids IgE is a better marker than egg white–specific IgE to diagnose boiled egg allergy

2012 ◽  
Vol 129 (6) ◽  
pp. 1681-1682 ◽  
Author(s):  
Yasuhiro Haneda ◽  
Naoyuki Kando ◽  
Masahiro Yasui ◽  
Takae Kobayashi ◽  
Toru Maeda ◽  
...  
Keyword(s):  
Specific Ige ◽  
Egg White ◽  
Egg Allergy
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