Effects of Buoyancy-Driven Convection on Nucleation and Growth of Protein Crystals

2004 ◽  
Vol 1027 (1) ◽  
pp. 1-9 ◽  
Author(s):  
CHRISTO N. NANEV ◽  
ANITA PENKOVA ◽  
NAOMI CHAYEN
2018 ◽  
Vol 74 (12) ◽  
pp. 1208-1218 ◽  
Author(s):  
Thomas Lykke-Møller Sørensen ◽  
Samuel John Hjorth-Jensen ◽  
Esko Oksanen ◽  
Jacob Lauwring Andersen ◽  
Claus Olesen ◽  
...  

Neutron macromolecular crystallography (NMX) has the potential to provide the experimental input to address unresolved aspects of transport mechanisms and protonation in membrane proteins. However, despite this clear scientific motivation, the practical challenges of obtaining crystals that are large enough to make NMX feasible have so far been prohibitive. Here, the potential impact on feasibility of a more powerful neutron source is reviewed and a strategy for obtaining larger crystals is formulated, exemplified by the calcium-transporting ATPase SERCA1. The challenges encountered at the various steps in the process from crystal nucleation and growth to crystal mounting are explored, and it is demonstrated that NMX-compatible membrane-protein crystals can indeed be obtained.


1997 ◽  
Vol 173 (1-2) ◽  
pp. 231-234 ◽  
Author(s):  
G. Sazaki ◽  
E. Yoshida ◽  
H. Komatsu ◽  
T. Nakada ◽  
S. Miyashita ◽  
...  

2018 ◽  
Vol 52 (16) ◽  
pp. 2132-2134
Author(s):  
A. S. Sokolovskii ◽  
N. A. Besedina ◽  
Yu. Berdnikov ◽  
V. G. Dubrovskii

2002 ◽  
Vol 13 (3) ◽  
pp. 14-21 ◽  
Author(s):  
L. Carotenuto ◽  
J. H. E. Cartwright ◽  
D. Castagnolo ◽  
J. M. García Ruiz ◽  
F. Otálora

1999 ◽  
Vol 11 (1) ◽  
pp. 61-62 ◽  
Author(s):  
Yukio NAGATA ◽  
Junichi MATSUSITA ◽  
Yuko INATOMI

2015 ◽  
Vol 15 (12) ◽  
pp. 5656-5660 ◽  
Author(s):  
Andrey Bogorodskiy ◽  
Fedor Frolov ◽  
Alexey Mishin ◽  
Ekaterina Round ◽  
Vitaly Polovinkin ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document