AbstractFluorescence microscopy methods are described with high spatial, spectral, and temporal resolution. In addition to three-dimensional (3D) microscopy, based on confocal, structured, or single-plane illumination, spectral imaging and fluorescence lifetime imaging microscopy are used to probe the conformation of fluorescent molecules as well as their interaction with the microenvironment. In addition to single cells or cell monolayers, 3D cell cultures are used increasingly, as they are more representative for tissue morphology and function. All methods are discussed in the context of controlled light exposure, which is regarded as a key parameter to maintain cell viability. The applications presented in this mini review include autofluorescence measurements of glioblastoma cells as well as various fluorescent markers or fluorescent proteins.
Multidimensional Fluorescence Microscopy for Simultaneous Functional and Structural Imaging
