α-Melanocyte-Stimulating Hormone-Like Peptides in the Intermediate Lobe of the Rat Pituitary Gland: Characterization of Content and Release in Vitro

Endocrinology ◽  
1983 ◽  
Vol 112 (2) ◽  
pp. 435-441 ◽  
Author(s):  
M. E. GOLDMAN ◽  
M. BEAULIEU ◽  
J. W. KEBABIAN ◽  
R. L. ESKAY
Keyword(s):  
Pituitary Gland ◽  
Intermediate Lobe ◽  
Melanocyte Stimulating Hormone ◽  
Rat Pituitary ◽  
Release In Vitro ◽  
Stimulating Hormone

ABILITY OF VARIOUS FACTORS TO OPPOSE THE STIMULATORY EFFECT OF DIBUTYRYL CYCLIC AMP ON THE RELEASE OF MELANOCYTE-STIMULATING HORMONE BY THE RAT PITUITARY IN VITRO

1976 ◽  
Vol 68 (2) ◽  
pp. 283-287 ◽  
Author(s):  
BRIDGET I. BAKER
Keyword(s):  
Cyclic Amp ◽  
Cyclic Gmp ◽  
Inhibitory Effect ◽  
Aminobutyric Acid ◽  
Intermediate Lobe ◽  
Dibutyryl Cyclic Amp ◽  
Melanocyte Stimulating Hormone ◽  
Rat Pituitary ◽  
Stimulating Hormone

SUMMARY Various agents were tested for their ability to oppose the stimulatory effect of dibutyryl cyclic AMP on the release of the melanocyte-stimulating hormone from the rat neuro-intermediate lobe in vitro. Only dopamine exhibited an inhibitory effect; serotonin, γ-aminobutyric acid, tocinoic acid, tocinamide, the tripeptide Pro-Leu-Gly-NH2 and dibutyryl cyclic GMP were all ineffective.

DIFFERENCES IN THE RELEASE OF MELANOCYTE-STIMULATING HORMONE IN VITRO BY RAT PITUITARY GLANDS COLLECTED AT VARIOUS TIMES DURING THE OESTROUS CYCLE

1978 ◽  
Vol 78 (1) ◽  
pp. 1-6 ◽  
Author(s):  
ADRIANA VIVAS ◽  
MARÍA ESTER CELIS
Keyword(s):  
Pituitary Gland ◽  
Oestrous Cycle ◽  
Biological Assay ◽  
Melanocyte Stimulating Hormone ◽  
Pituitary Tissue ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Stimulating Hormone

The release of melanocyte-stimulating hormone (MSH) into the medium during incubation and the pituitary tissue content of MSH were measured separately using pituitary glands collected from rats at various stages of the oestrous cycle. The MSH was measured by a biological assay using a synthetic α-MSH as standard. The release of MSH was maximal during the pro-oestrous phase and MSH content of the pituitary gland was highest during dioestrus. The influences of the tripeptide Pro-Leu-Gly-NH2, which inhibits MSH secretion in vivo, and of progesterone on the release of MSH in vitro were studied with tissue collected at various phases of the oestrous cycle. Pro-Leu-Gly-NH2 was effective in inhibiting MSH release both at pro-oestrus and oestrus but not at dioestrus. Progesterone overcame this inhibition.

INCORPORATION OF 14C-LABELLED AMINO ACIDS INTO CORTICOTROPHIN-LIKE INTERMEDIATE LOBE PEPTIDE AND α-MELANOCYTE-STIMULATING HORMONE BY THE RAT PITUITARY NEUROINTERMEDIATE LOBE IN VITRO, AND THE IDENTIFICATION OF FOUR NEW PARS INTERMEDIA PEPTIDES

1976 ◽  
Vol 70 (2) ◽  
pp. 197-205 ◽  
Author(s):  
A. P. SCOTT ◽  
P. J. LOWRY ◽  
TJ. B. VAN WIMERSMA GREIDANUS
Keyword(s):  
Amino Acids ◽  
The Other ◽  
Pars Intermedia ◽  
Intermediate Lobe ◽  
Melanocyte Stimulating Hormone ◽  
Neurointermediate Lobe ◽  
Rat Pituitary ◽  
Stimulating Hormone

SUMMARY At least seven radioactive peptides, which fractionated on Biogel P6, were found in rat neurointermediate lobes after incubation for 6 h with [14C]proline. Only three of these could be tentatively identified; one as α-melanocyte-stimulating hormone (α-MSH) and two as forms of corticotrophin-like intermediate lobe peptide (CLIP). One other crossreacted partially with a β-melanocyte-stimulating hormone (β-MSH) antiserum, was acidically charged and eluted on Biogel P6 in roughly the same position as ACTH. The other three peptides showed no resemblance to α-MSH, CLIP, β-MSH or ACTH.

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