EFFECTS OF CHOLINE ON THE LEUCOCYTE PICTURE AND ON THE SERUM CHOLINESTERASE ACTIVITY IN RABBITS

Abstract We describe a useful technique to identify "fluoride-resistant" serum cholinesterase variants (E1uE1f and E1fE1f) by using the method of Garry to detect "atypical" variants and to measure serum cholinesterase activity [Clin. Chem. 17, 192 (1971)]. With butyrylthiocholine as substrate, sodium fluoride is used as a differential inhibitor in both Tris and phosphate buffer systems (50 mmol/liter, pH 7.4, 25°C). Fluoride inhibition values are plotted on two-dimensional graph paper, Tris plus sodium fluoride vs. phosphate plus sodium fluoride. Inhibition values for the "fluoride-resistant" and "atypical" variants are located in specific quadrants, which permits unequivocal identification of each variant. Examination of 836 Caucasian preschool children revealed a gene frequency of 0.0066 for the "fluoride" variant. No "fluoride-resistant" variants were found in 168 Negro preschool children examined.

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Serum Cholinesterase in Analbuminemia

Clinical Chemistry ◽

10.1093/clinchem/12.1.25 ◽

1966 ◽

Vol 12 (1) ◽

pp. 25-27 ◽

Cited By ~ 11

Author(s):

A A Dietz ◽

H M Rubinstein ◽

T Lubrano

Keyword(s):

Cholinesterase Activity ◽

Serum Cholinesterase ◽

Intimate Connection ◽

Serum Cholinesterase Activity

Abstract Serum cholinesterase activity was found to be normal in persons with analbuminemia. This finding disproves previous speculations that there is some intimate connection between the hepatic biosynthesis of albumin and serum cholinesterase.

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A longitudinal study of cholinesterase changes in pregnancy.

Clinical Chemistry ◽

10.1093/clinchem/34.11.2249 ◽

1988 ◽

Vol 34 (11) ◽

pp. 2249-2252 ◽

Cited By ~ 19

Author(s):

R T Evans ◽

J O'Callaghan ◽

A Norman

Keyword(s):

Alcohol Intake ◽

Cholinesterase Activity ◽

Statistical Significance ◽

Smoking History ◽

Serum Cholinesterase ◽

Age Group ◽

Age Related ◽

Changes In Pregnancy ◽

Serum Cholinesterase Activity ◽

Increased Activity

Abstract Serum cholinesterase activity was measured at intervals before, during, and after 44 pregnancies in 43 women. Three patterns of change were seen: (a) A decline in activity after conception, with no return towards pre-conception values before delivery (20 pregnancies). (b) A decline in activity accompanied by a partial or complete return to pre-conception values before delivery (19 pregnancies). (c) Either no discernible decline or increased activity during gestation (five pregnancies). We saw no association between these patterns and either the sex of the child, parity, smoking history, or alcohol intake. However, the continuous decrease in cholinesterase activity occurred in the youngest group of women, a decrease followed by an increase in the intermediate age group, and no decrease at all in the oldest group. Although not all the age-related differences quite reached statistical significance, these findings suggest that the patterns of change may be determined by some aspect of maternal physical maturity.

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