scholarly journals Microbial ecology of Bacillus thuringiensis. VII. Fate of Bacillus thuringiensis in larvae of the silkworm, Bombyx mori, and the fall webworm, Hyphantria cunea.

1986 ◽  
Vol 30 (2) ◽  
pp. 99-105 ◽  
Author(s):  
Yoshio AKIBA
Keyword(s):  
Bacillus Thuringiensis ◽  
Microbial Ecology ◽  
Bombyx Mori ◽  
Hyphantria Cunea ◽  
Fall Webworm ◽  
Silkworm Bombyx Mori

scholarly journals Degradation of rRNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses

2013 ◽  
Vol 94 (9) ◽  
pp. 2102-2111 ◽  
Author(s):  
Rina Hamajima ◽  
Yuya Ito ◽  
Haruka Ichikawa ◽  
Hiroshi Mitsutake ◽  
Jun Kobayashi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Spodoptera Exigua ◽  
Autographa Californica ◽  
Cosmid Library ◽  
S2 Cells ◽  
Abortive Infection ◽  
Hyphantria Cunea ◽  
Transient Expression Assay ◽  
Rrna Degradation ◽  
Silkworm Bombyx Mori

Cell lines derived from the silkworm, Bombyx mori, are only permissive for B. mori nucleopolyhedrovirus (NPV), with other NPVs generally resulting in abortive infection. Here, we demonstrate that rRNA of B. mori BM-N cells undergoes rapid degradation through site-specific cleavage upon infection with NPVs from Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua (SeMNPV) and Spodoptera litura (SpltMNPV). No significant decreases in cellular RNA were observed in Ld652Y, Se301, Sf9, SpIm and S2 cells infected with AcMNPV or HycuMNPV, indicating the response is unique to BM-N cells. A transient expression assay using a cosmid library of the HycuMNPV genome demonstrated that HycuMNPV P143 is responsible for rRNA degradation, which was also detected in BM-N cells transfected with plasmids expressing the P143 proteins from AcMNPV, SeMNPV and SpltMNPV. These results indicate that B. mori evolved to acquire a unique antiviral immune mechanism that is activated by P143 proteins from heterologous NPVs.

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