Multiphoton Light-sheet Microscopy at Optimal Pulse Frequency for Fast In Vivo Imaging

2020 ◽  
Author(s):  
Vincent Maioli ◽  
Antoine Boniface ◽  
Pierre Mahou ◽  
Júlia Ferrer Ortas ◽  
Lamiae Abdeladim ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Pulse Frequency ◽  
Light Sheet Microscopy

Fast in vivo imaging using multiphoton light-sheet microscopy at optimal pulse frequency

2021 ◽  
Author(s):  
Vincent Maioli ◽  
Antoine Boniface ◽  
Pierre Mahou ◽  
Júlia Ferrer Ortas ◽  
Lamiae Abdeladim ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Pulse Frequency ◽  
Light Sheet Microscopy

Functional in vivo imaging using fluorescence lifetime light sheet microscopy (Conference Presentation)

2019 ◽  
Author(s):  
Simon M. Ameer-Beg ◽  
Claire A. Mitchell ◽  
Simon P. Poland ◽  
Robert D. Knight ◽  
Guoqing Wang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 1036-1049 ◽  
Author(s):  
Guy Malkinson ◽  
Pierre Mahou ◽  
Élodie Chaudan ◽  
Thierry Gacoin ◽  
Ali Y. Sonay ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Functional in vivo imaging using fluorescence lifetime light-sheet microscopy

2017 ◽  
Vol 42 (7) ◽  
pp. 1269 ◽  
Author(s):  
Claire A. Mitchell ◽  
Simon P. Poland ◽  
James Seyforth ◽  
Jakub Nedbal ◽  
Thomas Gelot ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency

2020 ◽  
Vol 11 (10) ◽  
pp. 6012 ◽  
Author(s):  
Vincent Maioli ◽  
Antoine Boniface ◽  
Pierre Mahou ◽  
Júlia Ferrer Ortas ◽  
Lamiae Abdeladim ◽  
...  
Keyword(s):  
Light Sheet ◽  
Pulse Frequency ◽  
Light Sheet Microscopy

scholarly journals Balancing signal and photoperturbation in multiphoton light-sheet microscopy by optimizing laser pulse frequency

2020 ◽  
Author(s):  
Vincent Maioli ◽  
Antoine Boniface ◽  
Pierre Mahou ◽  
Júlia Ferrer Ortas ◽  
Lamiae Abdeladim ◽  
...  
Keyword(s):  
Laser Pulse ◽  
Multiphoton Microscopy ◽  
Light Sheet ◽  
Pulse Frequency ◽  
Heart Beat ◽  
Research Field ◽  
Tissue Imaging ◽  
Power Pulse ◽  
Light Sheet Microscopy

AbstractImproving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to balance signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new opportunities for fast live tissue imaging.

Adaptive light sheet microscopy for in vivo imaging of fluorescently labeled specimens

2021 ◽  
Author(s):  
Mikis Mylonakis ◽  
Athanasios Zacharopoulos ◽  
Evangelos Marakis ◽  
Dimitrios G. Papazoglou ◽  
Giannis Zacharakis
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy
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