scholarly journals The Interaction between AID and CIB1 Is Nonessential for Antibody Gene Diversification by Gene Conversion or Class Switch Recombination

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11660 ◽  
Author(s):  
Zachary L. Demorest ◽  
Donna A. MacDuff ◽  
William L. Brown ◽  
Scott G. Morham ◽  
Leslie V. Parise ◽  
...  
Keyword(s):  
Gene Conversion ◽  
Class Switch Recombination ◽  
Class Switch ◽  
Gene Diversification ◽  
Antibody Gene

scholarly journals AID Mediates Hypermutation by Deaminating Single Stranded DNA

2003 ◽  
Vol 197 (10) ◽  
pp. 1291-1296 ◽  
Author(s):  
Sarah K. Dickerson ◽  
Eleonora Market ◽  
Eva Besmer ◽  
F. Nina Papavasiliou
Keyword(s):  
Gene Conversion ◽  
Somatic Hypermutation ◽  
Class Switch Recombination ◽  
Functional Link ◽  
Single Stranded Dna ◽  
Class Switch ◽  
Mutational Hotspots ◽  
Activation Induced Deaminase ◽  
Precise Role

Activation-induced deaminase (AID) is a protein indispensable for the diversification of immunoglobulin (Ig) genes by somatic hypermutation (SHM), class switch recombination (CSR), and gene conversion. To date, the precise role of AID in these processes has not been determined. Here we demonstrate that purified, tetrameric AID can deaminate cytidine residues in DNA, but not in RNA. Furthermore, we show that AID will bind and deaminate only single-stranded DNA, which implies a direct, functional link between hypermutation and transcription. Finally, AID does not target mutational hotspots, thus mutational targeting to specific residues must be attributed to different factors.

scholarly journals C-Terminal Deletion of AID Uncouples Class Switch Recombination from Somatic Hypermutation and Gene Conversion

2003 ◽  
Vol 12 (2) ◽  
pp. 501-508 ◽  
Author(s):  
Vasco Barreto ◽  
Bernardo Reina-San-Martin ◽  
Almudena R. Ramiro ◽  
Kevin M. McBride ◽  
Michel C. Nussenzweig
Keyword(s):  
Gene Conversion ◽  
Somatic Hypermutation ◽  
Class Switch Recombination ◽  
Terminal Deletion ◽  
Class Switch
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