Improved Development of Somatic Cell Cloned Mouse Embryos by Vitamin C and Latrunculin A

SummarySomatic cell nuclear transfer (SCNT) is an important technique for life science research. However, most SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we show that abnormal Xi occurs in somatic cell NT blastocysts, whereas in female blastocysts derived from cumulus cell nuclear transfer, both X chromosomes were inactive. H3K27me3 removal by Kdm6a mRNA overexpression could significantly improve preimplantation development of NT embryos, and even reached a 70.2% blastocyst rate of cleaved embryos compared with the 38.5% rate of the control. H3K27me3 levels were significantly reduced in blastomeres from cloned blastocysts after overexpression of Kdm6a. qPCR indicated that rDNA transcription increased in both NT embryos and 293T cells after overexpression of Kdm6a. Our findings demonstrate that overexpression of Kdm6a improved the development of cloned mouse embryos by reducing H3K27me3 and increasing rDNA transcription.

Download Full-text

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Journal of Visualized Experiments ◽

10.3791/57036 ◽

2018 ◽

Cited By ~ 2

Author(s):

Rika Azuma ◽

Kei Miyamoto ◽

Mami Oikawa ◽

Masayasu Yamada ◽

Masayuki Anzai

Keyword(s):

Vitamin C ◽

Somatic Cell ◽

Somatic Cell Nuclear Transfer ◽

Nuclear Transfer ◽

Trichostatin A ◽

Combinational Treatment

Download Full-text

Improving the development of early bovine somatic-cell nuclear transfer embryos by treating adult donor cells with vitamin C

Molecular Reproduction and Development ◽

10.1002/mrd.22531 ◽

2015 ◽

Vol 82 (11) ◽

pp. 867-879 ◽

Cited By ~ 12

Author(s):

Huanhuan Chen ◽

Lei Zhang ◽

Zekun Guo ◽

Yongsheng Wang ◽

Rongjun He ◽

...

Keyword(s):

Vitamin C ◽

Somatic Cell ◽

Somatic Cell Nuclear Transfer ◽

Nuclear Transfer ◽

Donor Cells

Download Full-text

Ascorbic acid in epigenetic reprogramming

Current Stem Cell Research & Therapy ◽

10.2174/1574888x16666210714152730 ◽

2021 ◽

Vol 16 ◽

Author(s):

Xinhui Liu ◽

Aamir Khan ◽

Huan Li ◽

Shensen Wang ◽

Xuechai Chen ◽

...

Keyword(s):

Ascorbic Acid ◽

Vitamin C ◽

Somatic Cell ◽

Epigenetic Modification ◽

Critical Role ◽

Cellular Reprogramming ◽

Epigenetic Reprogramming ◽

Cell Reprogramming ◽

Histone Demethylases ◽

Somatic Cell Reprogramming

: Emerging evidence suggests that ascorbic acid (vitamin C) enhances the reprogramming process by multiple mechanisms. This is primarily due to its cofactor role in Fe(II) and 2-oxoglutarate-dependent dioxygenases, including the DNA demethylases Ten Eleven Translocase (TET) and histone demethylases. Epigenetic variations have been shown to play a critical role in somatic cell reprogramming. DNA methylation and histone methylation are extensively recognized as barriers to somatic cell reprogramming. N6-methyladenosine (m6A), known as RNA methylation, is an epigenetic modification of mRNAs and has also been shown to play a role in regulating cellular reprogramming. Multiple cofactors are reported to promote the activity of demethylases, including vitamin C. This review focuses on examining the evidence and mechanism of vitamin C in DNA and histone demethylation and highlights its potential involvement in regulating m6A demethylation. It also shows the significant contribution of vitamin C in epigenetic regulation and the affiliation of demethylases with vitamin C-facilitated epigenetic reprogramming.

Download Full-text