scholarly journals Nucleotide diversity based on csd gene of the black giant honey bee, Apis laboriosa (Hymenoptera: Apidae)

2013 ◽  
Vol 110 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Huan WANG ◽  
Zi-Long WANG ◽  
Zhi-Jiang ZENG ◽  
Xiao-Bo WU ◽  
YAN Wei-Yu
2015 ◽  
Vol 27 (5) ◽  
pp. 3755-3756 ◽  
Author(s):  
Liansangmawii Chhakchhuak ◽  
Surajit De Mandal ◽  
Guruswami Gurusubramanian ◽  
Naganeeswaran Sudalaimuthu ◽  
Chellappa Gopalakrishnan ◽  
...  

Insects ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 868
Author(s):  
Samuele Bovo ◽  
Anisa Ribani ◽  
Valerio Joe Utzeri ◽  
Valeria Taurisano ◽  
Giuseppina Schiavo ◽  
...  

The complementary sex determiner (csd) gene plays an essential role in the sex determination of Apis mellifera L. Females develop only if fertilized eggs have functional heterozygous genotypes at this gene whereas males, being haploids, are hemizygous. Two identical csd alleles produce non vital males. In light of the recent decline in honey bee populations, it is therefore important to monitor the allele variability at this gene. In this study, we tested the application of next generation semiconductor-based sequencing technology (Ion Torrent) coupled with environmental honey DNA as a source of honey bee genome information to retrieve massive sequencing data for the analysis of variability at the hypervariable region (HVR) of the csd gene. DNA was extracted from 12 honey samples collected from honeycombs directly retrieved from 12 different colonies. A specifically designed bioinformatic pipeline, applied to analyze a total of about 1.5 million reads, identified a total of 160 different csd alleles, 55% of which were novel. The average number of alleles per sample was compatible with the number of expected patrilines per colony, according to the mating behavior of the queens. Allele diversity at the csd could also provide information useful to reconstruct the history of the honey.


2011 ◽  
Vol 18 (6) ◽  
pp. 645-651 ◽  
Author(s):  
Zhi-Yong Liu ◽  
Zi-Long Wang ◽  
Xiao-Bo Wu ◽  
Wei-Yu Yan ◽  
Zhi-Jiang Zeng

ZooKeys ◽  
2020 ◽  
Vol 951 ◽  
pp. 67-81
Author(s):  
Nyaton Kitnya ◽  
M. V. Prabhudev ◽  
Chet Prasad Bhatta ◽  
Thai Hong Pham ◽  
Tshering Nidup ◽  
...  

Worldwide pollinator declines have dramatically increased our need to survey and monitor pollinator distributions and abundances. The giant honey bee, Apis laboriosa, is one of the important pollinators at higher altitudes of the Himalayas. This species has a restricted distribution along the Himalayas and neighbouring mountain ranges of Asia. Previous assessments of its distribution, published more than 20 years ago, were based on museum specimens. Since then, 244 additional localities have been revealed through field trips by the authors, publications, and websites. We present a revised distribution for A. laboriosa that better defines its range and extends it eastward to the mountains of northern Vietnam, southward along the Arakan Mountains to west-central Myanmar, into the Shillong Hills of Meghalaya, India, and northwestward in Uttarakhand, India. This species is generally found at elevations between 1000–3000 m a.s.l.. In northeastern India A. laboriosa colonies occur during summer at sites as low as 850 m a.s.l. and some lower elevation colonies maintain their nests throughout the winter. Finally, we report three regions in Arunachal Pradesh, India, and nine locations in northern Vietnam, where we observed workers of A. laboriosa and A. dorsata foraging sympatrically; their co-occurrence supports the species status of Apis laboriosa.


Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 483 ◽  
Author(s):  
Éva Kolics ◽  
Tamás Parrag ◽  
Ferenc Házi ◽  
Kinga Szepesi ◽  
Botond Heltai ◽  
...  

Applying instrumental insemination in closely related honey bee colonies often leads to frequent lethality of offspring causing colony collapse. This is due to the peculiarities of honey bee reproductive biology, where the complementary sex determination (csd) gene drives sex determination within a haplodiploid system. Diploid drones containing homozygous genotypes are lethal. Tracking of csd alleles using molecular markers prevents this unwanted event in closed breeding programs. Our approach described here is based on high throughput sequencing (HTS) that provides more data than traditional molecular techniques and is capable of analysing sources containing multiple alleles, including diploid individuals as the bee queen. The approach combines HTS technique and clipping wings as a minimally invasive method to detect the complementary sex determiner (csd) alleles directly from honey bee queens. Furthermore, it might also be suitable for screening alleles of honey harvested from hives of a closed breeding facility. Data on alleles of the csd gene from different honey bee subspecies are provided. It might contribute to future databases that could potentially be used to track the origin of honey. With the help of tracking csd alleles, more focused crossings will be possible, which could in turn accelerate honey bee breeding programmes targeting increase tolerance against varroosis as well.


EDIS ◽  
2020 ◽  
Vol 2020 (1) ◽  
pp. 7
Author(s):  
Catherine M. Mueller ◽  
Cameron Jack ◽  
Ashley N. Mortensen ◽  
Jamie D. Ellis

European foulbrood is a bacterial disease that affects Western honey bee larvae. It is a concern to beekeepers everywhere, though it is less serious than American foulbrood because it does not form spores, which means that it can be treated. This 7-page fact sheet written by Catherine M. Mueller, Cameron J. Jack, Ashley N. Mortensen, and Jamie Ellis and published by the UF/IFAS Entomology and Nematology Department describes the disease and explains how to identify it to help beekeepers manage their colonies effectively and prevent the spread of both American and European foulbrood.https://edis.ifas.ufl.edu/in1272


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