scholarly journals Mitotic activity in gastrointestinal stromal tumors: Can we use phosphohistone H3 immunohistochemistry instead of HE for mitotic count?

2021 ◽  
Author(s):  
Selma Sengiz Erhan
Keyword(s):  
Mitotic Activity ◽  
Gastrointestinal Stromal Tumors ◽  
Mitotic Count ◽  
Stromal Tumors ◽  
Phosphohistone H3

Gastrointestinal Stromal Tumors: A “Benign” Tumor with Hepatic Metastasis after 11 Years

1998 ◽  
Vol 84 (1) ◽  
pp. 78-81 ◽  
Author(s):  
Carlo Ballarini ◽  
Mattia Intra ◽  
Andrea Pisani Ceretti ◽  
Francesco Prestipino ◽  
Filippo Maria Bianchi ◽  
...  
Keyword(s):  
Surgical Treatment ◽  
Hepatic Metastasis ◽  
Tumor Size ◽  
Gastrointestinal Stromal Tumors ◽  
Cellular Proliferation ◽  
Malignant Potential ◽  
Mitotic Count ◽  
Proliferation Index ◽  
Ki 67 ◽  
Stromal Tumors

Gastrointestinal stromal tumors (GIST) constitue the largest category of primary non-epithelial neoplasms of the stomach and small bowel. They are characterized by a remarkable cellular variability and their malignant potential is sometimes difficult to predict. Very recent studies, using mitotic count and tumor size as the best determinants of biological behavior, divide GISTs into three groups: benign, borderline and malignant tumors. We report on a male patient who underwent a right hepatectomy for a large metastasis 11 years after the surgical treatment of an antral-pyloric gastric neoplasm, histologically defined as leiomyoblastoma and with clinical, morphological and immunohistochemical features of benignity (low mitotic count, tumor size < 5 cm, low cellular proliferation index). Histological and immunohistochemical analysis of the hepatic metastasis showed the cellular proliferation index (Ki-67) to be positive in 25% of neoplastic cells, as opposed to the primary gastric tumor in which Ki-67 was positive in only 5% of neoplastic cells. In conclusion, although modern immunohistochemical techniques are now available to obtain useful prognostic information, the malignant potential of GISTs is sometimes difficult to predict: neoplasms clinically and histologically defined as benign could metastasize a long time after oncologically correct surgical treatment. Therefore, benign GISTs also require consistent, long-term follow-up.

scholarly journals Building CT Radiomics-Based Models for Preoperatively Predicting Malignant Potential and Mitotic Count of Gastrointestinal Stromal Tumors

2019 ◽  
Vol 12 (9) ◽  
pp. 1229-1236 ◽  
Author(s):  
Chao Wang ◽  
Hailin Li ◽  
Yeerfan Jiaerken ◽  
Peiyu Huang ◽  
Lifeng Sun ◽  
...  
Keyword(s):  
Gastrointestinal Stromal Tumors ◽  
Malignant Potential ◽  
Mitotic Count ◽  
Stromal Tumors

scholarly journals Phosphohistone H3 Immunohistochemical Labeling: a Potentially Useful Tool for Risk Stratification and Prognostic Analysis in Gastrointestinal Stromal Tumors.

2021 ◽  
Author(s):  
Xiaohong Li ◽  
Yutao Zhang ◽  
Feng Li ◽  
Yun Tang ◽  
Hongyuan Zhou
Keyword(s):  
Risk Stratification ◽  
Gastrointestinal Stromal Tumors ◽  
Ki67 Index ◽  
Stromal Tumors ◽  
Prognostic Analysis ◽  
Phosphohistone H3 ◽  
Primary Location ◽  
Risk Grade ◽  
Positive Index ◽  
Expression Status

Abstract BackgroundIt is well recognized that risk stratification of gastrointestinal stromal tumors (GISTs) is closely related to tumor size, mitotic index (MI), and primary location. Among these three parameters, tumor size and primary location are easily established, while MI is subjective and its repeatability is poor. It is thus necessary to identify a biomarker to represent the true MI. Expression status and biological or prognostic significance of mitotic marker phosphohistone H3 (PHH3) and cell proliferation marker Ki67 in GIST have not been clearly understood until now. MethodsAn immunohistochemistry experiment was performed to detect the expression status of PHH3 and Ki67 in 125 paraffin-embedded GIST samples. All of the patients were followed up until September 30, 2019. ResultsThe MI determined using stained hematoxylin and eosin (H&E) sections (MI-H&E) and immunohistochemically positive PHH3 index (PHH3-IHC) was compared among groups of different genders, ages, primary locations, and histological subtypes, showing that the difference was not statistically significant (P>0.05). MI-H&E and the immunohistochemically positive Ki67 index were positively correlated (r=0.273, P=0.001), but the correlation was lower than that with the PHH3-positive index (r=0.705, P=0.000). The PHH3-positive index was also positively correlated with the Ki67 index (r=0.224, P=0.006). MI-H&E were positively correlated with MI quantified using immunohistochemically stained PHH3 sections (MI-PHH3) (P<0.05). After using PHH3 to perform MI quantification, the risk stratification of five GIST cases was changed, where two cases were given a higher risk grade and three cases were given a lower risk grade. Follow-up data were obtained from 98 cases (98/125, 78.4%), including two cases of metastasis and one death. Both metastatic and death cases had high MI-H&E. One metastatic case and one death case had high PHH3-positive indexes, while the remaining metastatic case had a low PHH3-positive index. ConclusionImmunohistochemical PHH3 labeling is a potentially useful tool for risk stratification and prognostic analysis in GIST. Using immunohistochemical PHH3 labeling makes it more convenient for pathologists to determine the MI for GIST. MI quantification with immunohistochemical PHH3 sections can be used as an adjunct tool for risk stratification and prognostic analysis of GIST, but cannot completely replace MI quantification using stained H&E sections. The Ki67 index is positively correlated with MI-H&E, although the efficiency of tumor risk stratification is lower than that of PHH3.

Usefulness of anti-phosphohistone H3 immunoreactivity to determine mitotic rate in gastrointestinal stromal tumors

2012 ◽  
Vol 5 (4) ◽  
pp. 91-97 ◽  
Author(s):  
Ahrong Kim ◽  
Dong Han Im ◽  
Kyungbin Kim ◽  
Jee Yeon Kim ◽  
Mee Young Sol ◽  
...  
Keyword(s):  
Gastrointestinal Stromal Tumors ◽  
Mitotic Rate ◽  
Stromal Tumors ◽  
Phosphohistone H3

Surgical management of gastrointestinal stromal tumors: A single institution 31 — Year experience

2001 ◽  
Vol 120 (5) ◽  
pp. A401-A401 ◽  
Author(s):  
D EFRON ◽  
K LILLEMOE ◽  
J CAMERON ◽  
S TIERNEY ◽  
S ABRAHAM ◽  
...  
Keyword(s):  
Surgical Management ◽  
Gastrointestinal Stromal Tumors ◽  
Single Institution ◽  
Stromal Tumors
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