Interspecific Hybridization Of Red Clover (Trifolium Pratense L.) With Alsike Clover (Trifolium Hybndum L.) Using In Vitro Embryo Rescue

Establishing the effect of the amino acids as additional additives to the culture medium is and will be in the future one of our concerns of interest for the in vitro culture of some plants. The present study examines the effect of the glicocol added to the LS basal medium over the embryos of the Trifolium pratense L specie cultivated in vitro. There were followed: the percentage of plant regeneration of the red clover, its multiplication capacity and the formation of the root system, and also the evolution of the callus obtained on mediums with 2,4D, BA and amino acid.

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In vitro selection for aluminium tolerance in red clover (Trifolium pratense L.)

Plant-Soil Interactions at Low pH: Principles and Management ◽

10.1007/978-94-011-0221-6_68 ◽

1995 ◽

pp. 451-453

Author(s):

W. Pawtowski ◽

B. Skucińska

Keyword(s):

In Vitro Selection ◽

Trifolium Pratense ◽

Red Clover ◽

Aluminium Tolerance ◽

Trifolium Pratense L ◽

Selection For

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Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: An in vitro study in MCF-7 cells

Fitoterapia ◽

10.1016/j.fitote.2014.01.027 ◽

2014 ◽

Vol 94 ◽

pp. 62-69 ◽

Cited By ~ 20

Author(s):

Paola Spagnuolo ◽

Emanuela Rasini ◽

Alessandra Luini ◽

Massimiliano Legnaro ◽

Marcello Luzzani ◽

...

Keyword(s):

Trifolium Pratense ◽

Estrogenic Activity ◽

Red Clover ◽

In Vitro Study ◽

Vitro Study ◽

Isoflavone Content ◽

Trifolium Pratense L ◽

Mcf 7

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The role of medicarpin and maackiain in the response of red clover leaves to Helminthosporium carbonum, Stemphylium botryosum, and S. sarcinaeforme

Canadian Journal of Botany ◽

10.1139/b76-281 ◽

1976 ◽

Vol 54 (23) ◽

pp. 2609-2619 ◽

Cited By ~ 12

Author(s):

Lorne J. Duczek ◽

Verna J. Higgins

Keyword(s):

Trifolium Pratense ◽

Leaf Tissue ◽

Red Clover ◽

Stemphylium Botryosum ◽

Inhibitory Compounds ◽

Trifolium Pratense L ◽

The Difference

Helminthosporium carbonum, a corn pathogen, and Stemphylium botryosum, an alfalfa pathogen, are both nonpathogenic on red clover (Trifolium pratense L.), while S. sarcinaeforme is a foliar pathogen on red clover. In clover leaves challenged with H. carbonum, medicarpin and maackiain were the only inhibitory compounds found in diffusates or in leaf tissue in a concentration sufficient to account for the inhibition of this fungus. Helminthosporium carbonum was inhibited by and could not degrade medicaipin and (or) maackiain in vitro. Both S. botryosum and S. sarcinaeforme were only slightly inhibited by these compounds in mycelial growth bioassays, and both fungi degraded medicarpin and (or) maackiain in vitro and some evidence was obtained that degradation occurred in vivo. In contrast with the relatively high amounts of medicarpin and maackiain that accumulated in leaves challenged with H. carbonum, relatively low amounts accumulated in leaves challenged with either S. botryosum or S. sarcinaeforme. The evidence suggests that the resistance of clover to H. carbonum can be accounted for by these phytoalexins; however, differences in relation to accumulation of, inhibition by, and breakdown of medicarpin and (or) maackiain were not enough to explain the difference in pathogenicity of S. botryosum and S. sarcinaeforme on red clover.

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