scholarly journals The Role of Amino Acids for the In Vitro Culture of Some Species of Forage Legumes I. Trifolium pratense L

2013 ◽  
Vol 70 (1) ◽  
pp. 87-92
Author(s):  
Gabriela Maria VICAȘ ◽  
Mircea SAVATTI
Keyword(s):  
Amino Acids ◽  
In Vitro Culture ◽  
Culture Medium ◽  
Trifolium Pratense ◽  
Basal Medium ◽  
Red Clover ◽  
Forage Legumes ◽  
Trifolium Pratense L

Establishing the effect of the amino acids as additional additives to the culture medium is and will be in the future one of our concerns of interest for the in vitro culture of some plants. The present study examines the effect of the glicocol added to the LS basal medium over the embryos of the Trifolium pratense L specie cultivated in vitro. There were followed: the percentage of plant regeneration of the red clover, its multiplication capacity and the formation of the root system, and also the evolution of the callus obtained on mediums with 2,4D, BA and amino acid.

The role of medicarpin and maackiain in the response of red clover leaves to Helminthosporium carbonum, Stemphylium botryosum, and S. sarcinaeforme

1976 ◽  
Vol 54 (23) ◽  
pp. 2609-2619 ◽  
Author(s):  
Lorne J. Duczek ◽  
Verna J. Higgins
Keyword(s):  
Trifolium Pratense ◽  
Leaf Tissue ◽  
Red Clover ◽  
Stemphylium Botryosum ◽  
Inhibitory Compounds ◽  
Trifolium Pratense L ◽  
The Difference

Helminthosporium carbonum, a corn pathogen, and Stemphylium botryosum, an alfalfa pathogen, are both nonpathogenic on red clover (Trifolium pratense L.), while S. sarcinaeforme is a foliar pathogen on red clover. In clover leaves challenged with H. carbonum, medicarpin and maackiain were the only inhibitory compounds found in diffusates or in leaf tissue in a concentration sufficient to account for the inhibition of this fungus. Helminthosporium carbonum was inhibited by and could not degrade medicaipin and (or) maackiain in vitro. Both S. botryosum and S. sarcinaeforme were only slightly inhibited by these compounds in mycelial growth bioassays, and both fungi degraded medicarpin and (or) maackiain in vitro and some evidence was obtained that degradation occurred in vivo. In contrast with the relatively high amounts of medicarpin and maackiain that accumulated in leaves challenged with H. carbonum, relatively low amounts accumulated in leaves challenged with either S. botryosum or S. sarcinaeforme. The evidence suggests that the resistance of clover to H. carbonum can be accounted for by these phytoalexins; however, differences in relation to accumulation of, inhibition by, and breakdown of medicarpin and (or) maackiain were not enough to explain the difference in pathogenicity of S. botryosum and S. sarcinaeforme on red clover.

Étude de l'influence des réserves azotées dans l'estimation par 15N de la fixation symbiotique d'une plante pérenne, Trifolium pratense

1985 ◽  
Vol 63 (6) ◽  
pp. 991-994
Author(s):  
Chantal Lescure ◽  
Alain Chalamet
Keyword(s):  
Trifolium Pratense ◽  
Control Plant ◽  
Red Clover ◽  
Good Control ◽  
Dinitrogen Fixation ◽  
Dilution Technique ◽  
Trifolium Pratense L ◽  
Perennial Legumes ◽  
Kinetics Of

A 15N dilution technique is proposed to determine the role of nitrogen reserves in the plant on the estimation of dinitrogen fixation, during regrowth of perennial legumes. It is based on labelling of nitrogen compartments of the plant. Since the kinetics of utilization of nitrogen reserves of ryegrass (Lolium italicum L.) and red clover (Trifolium pratense L.) appear similar, ryegrass would be a good control plant. Despite this observation, the comparison of two methods (based on two or three sources of nitrogen) to estimate dinitrogen fixation shows the difficulty in applying the technique using 15N-labelled substrate over earlier periods of regrowth. In this case, the 15N method described for determining symbiotic dinitrogen fixation could be applied in controlled conditions.

Isoflavone content and estrogenic activity of different batches of red clover (Trifolium pratense L.) extracts: An in vitro study in MCF-7 cells

Fitoterapia ◽  
2014 ◽  
Vol 94 ◽  
pp. 62-69 ◽  
Author(s):  
Paola Spagnuolo ◽  
Emanuela Rasini ◽  
Alessandra Luini ◽  
Massimiliano Legnaro ◽  
Marcello Luzzani ◽  
...  
Keyword(s):  
Trifolium Pratense ◽  
Estrogenic Activity ◽  
Red Clover ◽  
In Vitro Study ◽  
Vitro Study ◽  
Isoflavone Content ◽  
Trifolium Pratense L ◽  
Mcf 7

scholarly journals Effect of intercropping wheat with forage legumes on wheat production and ground cover

2001 ◽  
Vol 36 (3) ◽  
pp. 465-471 ◽  
Author(s):  
Gilberto Omar Tomm ◽  
Robert Kerry Foster
Keyword(s):  
Grain Yield ◽  
Trifolium Pratense ◽  
Wheat Yield ◽  
Red Clover ◽  
Ground Cover ◽  
Soil Surface ◽  
Triticum Aestivum L ◽  
First Year ◽  
Forage Legumes ◽  
Trifolium Pratense L

The use of winter legumes in southern Brazil is hindered by the slow growth of these species during establishment exposing soil surface to erosion. Introduction of these species along with spring wheat (Triticum aestivum L.) was studied as a means of increasing ground cover during their initial establishment period, without reducing wheat grain yield. Two experiments were conducted in nearby areas, one in each year. Birdsfoot trefoil (Lotus corniculatus L.), red clover (Trifolium pratense L.) cultivar Quiñequelli, white clover (T. repens L.), and arrowleaf clover (T. vesiculosum Savi) did not reduce cereal yield in either year. Wheat yield was reduced by intercropped red clover cultivar Kenland and by subclover (T. subterraneum L.) in the first year. No grain yield differences due to intercropping with any legume were detected in the second year, when rainfall was below normal. Intercropping with wheat showed to be a practical alternative to enhance ground cover at establishing forage legumes.

Effect of changes in lipid classes during wilting and ensiling of red clover using two silage additives onin vitroruminal biohydrogenation

2016 ◽  
Vol 154 (3) ◽  
pp. 553-566 ◽  
Author(s):  
F. GADEYNE ◽  
K. DE RUYCK ◽  
G. VAN RANST ◽  
N. DE NEVE ◽  
B. VLAEMINCK ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Microbial Activity ◽  
Trifolium Pratense ◽  
Neutral Lipids ◽  
Red Clover ◽  
Control Distribution ◽  
Microbial Lipases ◽  
Sufficient Variation

SUMMARYAlthough forage lipid is generally rich in polyunsaturated fatty acids (PUFA), recovery of these fatty acids (FA) in milk and meat of ruminant origin is generally low, due to microbial biohydrogenation (BH) taking place in the rumen. Since lipolysis is a prerequisite for BH, the latter process is expected to be enhanced when (conserved) forages contain lower levels of esterified FA (particularly polar lipids; PL). However, this was not observed in former studies with red clover (Trifolium pratenseL.). Furthermore, red clover inclusion in the herbivore's diet was associated with decreased rumen BH as compared with other forages. Differences in plant lipase activity during wilting and ensiling has been attributed to changes in disappearance from the PL fraction, but a potential role of microbial lipasesin silohas not yet been elucidated. Therefore, the aims of the present study were to assess whether BH of red clover FA is linked with PL levels of the (conserved) starting material and to clarify the possible role ofin silomicrobial activity on PL disappearance. In order to obtain sufficient variation in forage PL and microbial activity, laboratory-scale silages were made by wilting and ensiling damaged or undamaged red clover using molasses or formic acid as ensiling additive, while perennial ryegrass (Lolium perenneL.) was used as a control. Distribution of lipids within three lipid fractions (PL, free FA and neutral lipids) in forages was determined and BH calculated after 24 hin vitrorumen incubation. Results indicated microbial lipases in silages did not enhance FA disappearance from the PL fraction. A gradual decrease of FA in the PL fraction upon conservation was found, both in red clover and ryegrass, irrespective of the degree of damage. In red clover PL losses started from the wilting phase, while substantial PL disappearance from ryegrass only started upon ensiling. Proportions of PUFA remaining in the PL fraction after wilting and ensiling of red clover were positively correlated with PUFA BH, while this effect was not observed for ryegrass. Red clover PUFA seemed to be partially protected against ruminal BH, while disappearance of FA from the PL fraction did not seem to be hampered. Results indicated the encapsulation mechanism as a consequence of protein-bound phenol formation induced by polyphenol oxidase is still the most probable hypothesis to explain red clover's increased flow of PUFA across the rumen.

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