Practice for Determination of Minimum Immersion Depth and Assessment of Temperature Sensor Measurement Drift

10.1520/d7962 ◽  
2015 ◽  
Author(s):  
Micromachines ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 370 ◽  
Author(s):  
Tatsuhiro Fukuba ◽  
Takuroh Noguchi ◽  
Kei Okamura ◽  
Teruo Fujii

Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L–L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10−11 M.


2012 ◽  
Vol 47 ◽  
pp. 611-614 ◽  
Author(s):  
D. Mrugala ◽  
F. Ziegler ◽  
J. Kostelnik ◽  
W. Lang

2001 ◽  
Vol 80 (3) ◽  
pp. 1406-1416 ◽  
Author(s):  
R. Scott Prosser ◽  
Paul A. Luchette ◽  
Philip W. Westerman ◽  
Annett Rozek ◽  
Robert E.W. Hancock

2007 ◽  
Vol 62 (9) ◽  
pp. 903-905 ◽  
Author(s):  
B. K. Zuev ◽  
V. I. Dvorkin ◽  
V. G. Filonenko ◽  
A. V. Mikhailova ◽  
I. A. Bubnova

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