scholarly journals Munc13-1 C1 Domain Activation Lowers the Energy Barrier for Synaptic Vesicle Fusion

2007 ◽  
Vol 27 (5) ◽  
pp. 1200-1210 ◽  
Author(s):  
J. Basu ◽  
A. Betz ◽  
N. Brose ◽  
C. Rosenmund
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Vesicle Fusion ◽  
C1 Domain ◽  
Synaptic Vesicle Fusion

scholarly journals Author response: Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate

2015 ◽  
Author(s):  
Sebastiaan Schotten ◽  
Marieke Meijer ◽  
Alexander Matthias Walter ◽  
Vincent Huson ◽  
Lauren Mamer ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Fusion Rate ◽  
Vesicle Fusion ◽  
Author Response ◽  
Additive Effects ◽  
Synaptic Vesicle Fusion

scholarly journals Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Vincent Huson ◽  
Marieke Meijer ◽  
Rien Dekker ◽  
Mirelle ter Veer ◽  
Marvin Ruiter ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Phorbol Esters ◽  
Release Kinetics ◽  
Vesicle Fusion ◽  
Tetanic Stimulation ◽  
Release Rates ◽  
Synaptotagmin 1 ◽  
Post Tetanic Potentiation ◽  
Synaptic Vesicle Fusion

Previously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and post-tetanic potentiation (PTP). We assess energy barrier changes by fitting release kinetics in response to hypertonic sucrose. Mimicking activation of the C2A domain of the Ca2+-sensor Synaptotagmin-1 (Syt1), by adding a positive charge (Syt1D232N) or increasing its hydrophobicity (Syt14W), lowers the energy barrier. Removing Syt1 or impairing its release inhibitory function (Syt19Pro) increases spontaneous release without affecting the fusion barrier. Both phorbol esters and tetanic stimulation potentiate synaptic strength, and lower the energy barrier equally well in the presence and absence of Syt1. We propose a model where tetanic stimulation activates Syt1-independent mechanisms that lower the energy barrier and act additively with Syt1-dependent mechanisms to produce PTP by exerting multiplicative effects on release rates.

scholarly journals Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1

2020 ◽  
Author(s):  
Vincent Huson ◽  
Marieke Meijer ◽  
Rien Dekker ◽  
Mirelle ter Veer ◽  
Marvin Ruiter ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Phorbol Esters ◽  
Release Kinetics ◽  
Vesicle Fusion ◽  
Tetanic Stimulation ◽  
Release Rates ◽  
Synaptotagmin 1 ◽  
Post Tetanic Potentiation ◽  
Synaptic Vesicle Fusion

AbstractPreviously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and post-tetanic potentiation (PTP). We assess energy barrier changes by fitting release kinetics in response to hypertonic sucrose. Mimicking activation of the C2A domain of the Ca2+-sensor Synaptotagmin-1 (Syt1), by adding a positive charge (Syt1D232N) or increasing its hydrophobicity (Syt14W), lowers the energy barrier. Removing Syt1 or impairing its release inhibitory function (Syt19Pro) increases spontaneous release without affecting the fusion barrier. Both phorbol esters and tetanic stimulation potentiate synaptic strength, and lower the energy barrier equally well in the presence and absence of Syt1. We propose a model where tetanic stimulation activates Syt1 dependent and independent mechanisms that lower the energy barrier independently in an additive manner to produce PTP by multiplication of release rates.

scholarly journals Correction: Additive effects on the energy barrier for synaptic vesicle fusion cause supralinear effects on the vesicle fusion rate

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Sebastiaan Schotten ◽  
Marieke Meijer ◽  
Alexander Matthias Walter ◽  
Vincent Huson ◽  
Lauren Mamer ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Fusion Rate ◽  
Vesicle Fusion ◽  
Additive Effects ◽  
Synaptic Vesicle Fusion
Sign in / Sign up

Export Citation Format

Share Document