RELIABILITY CRITERIA FOR SATURATION ANALYSIS OF STEROIDS BY COMPETITIVE PROTEIN BINDING

1970 ◽  
Vol 65 (1_Suppl) ◽  
pp. S61-S78 ◽  
Author(s):  
Billy D. Reeves ◽  
David W. Calhoun
Keyword(s):  
Protein Binding ◽  
Assay Method ◽  
Statistical Control ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
System 2 ◽  
Competitive Protein Binding ◽  
Single Method ◽  
Assay Design ◽  
Proper Perspective

ABSTRACT This communication is an attempt to delineate and define reliability criteria for saturation analysis of steroids by competitive protein binding assay. The discussion of these criteria evolved from three major considerations of assay method that help to place the ultimate criterion of accuracy in proper perspective. These major considerations are: 1) the measurement system, 2) the assay design and 3) the calculations and statistical control. Such an approach permits an evaluation, both relative and absolute, for a single method or for multiple methods.

A Simplified competitive protein-binding assay for 25-hydroxycalciferol

1976 ◽  
Vol 68 (2) ◽  
pp. 99-105 ◽  
Author(s):  
B. Garcia-Pascual ◽  
A. Peytremann ◽  
B. Courvoisier ◽  
D.E.M. Lawson
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1974 ◽  
Vol 46 (2) ◽  
pp. 231-240 ◽  
Author(s):  
S. Edelstein ◽  
M. Charman ◽  
D. E. M. Lawson ◽  
E. Kodicek
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Rat Serum ◽  
Male And Female ◽  
Normal Volunteers ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean ◽  
25 Hydroxycholecalciferol

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.

A competitive protein binding assay for urinary riboflavin

1974 ◽  
Vol 9 (2) ◽  
pp. 167-176 ◽  
Author(s):  
Arpad G. Fazekas ◽  
Carlos E. Menendez ◽  
Richard S. Rivlin
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding
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