IMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE (LH) IN URINE. A COMPARISON BETWEEN A HAEMAGGLUTINATION AND RADIOIMMUNOSORBENT METHOD

1971 ◽  
Vol 67 (3) ◽  
pp. 491-494 ◽  
Author(s):  
Bruce Hobson
Keyword(s):  
Luteinizing Hormone ◽  
Haemagglutination Inhibition ◽  
Inhibition Test ◽  
Urine Samples ◽  
Human Pituitary ◽  
Haemagglutination Inhibition Test

ABSTRACT A modified haemagglutination inhibition test (Luteonosticon) was used to estimate human pituitary LH in urines from 66 women and 2 men. The results were compared with those obtained by a radioimmunosorbent method on the same urine samples. The correlation between the results of the two methods was highly significant (P < 0.001).

A scheme for the identification of the blood of Australasian marsupials

1969 ◽  
Vol 17 (5) ◽  
pp. 799 ◽  
Author(s):  
JAW Kirsch ◽  
MD Murray
Keyword(s):  
Geographical Distribution ◽  
Haemagglutination Inhibition ◽  
Inhibition Test ◽  
Haemagglutination Inhibition Test ◽  
Blood Sucking ◽  
The Family ◽  
Precipitin Test ◽  
Marsupial Species

A collection of sera from 81 species of Australasian marsupials and 65 antisera to them, prepared in rabbits, chickens, or various species of marsupials, was examined to devise a scheme to identify the blood of marsupials imbibed by blood-sucking insects. The precipitin test was of limited value but established that the blood was marsupial. With the haemagglutination inhibition test, however, it was possible to identify the family, subfamily, genus, and species. Thus a procedure could be devised to identify the donors' blood based principally on serological methods supplemented by data on the geographical distribution of the various marsupial species.

scholarly journals The use of the haemagglutination-inhibition test in epidemiological influenza-virus studies

1949 ◽  
Vol 47 (4) ◽  
pp. 396-397 ◽  
Author(s):  
Hendrik L. Wolff
Keyword(s):  
Influenza Virus ◽  
Influenza Infection ◽  
Haemagglutination Inhibition ◽  
Inhibition Test ◽  
Haemagglutination Inhibition Test

The importance of the haemagglutination-inhibition test does not need to be stressed. It is a fact that the inhibition titre of a single serum has no constant value, but may vary in repeated estimations. This may be due to the use of different virus or erythrocyte suspensions or to differences in environmental circumstances, such as temperature. Duplicate tests carried out at the same time and with the same materials yield identical results. This makes it necessary to compare a serum obtained during convalescence from the same case. The ratio between the two titres shows whether an influenza infection has taken place or not. Although it is commonly accepted that a titre rising by two twofold dilutions (a fourfold rise) is significant, some workers will accept even a twofold rise (Sartwill & Long, 1948; Rasmussen, Stoles & Smadel, 1948). Such observations have been made on pairs of sera taken from the same indicidual with not more than a 5-week interval between them.

IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

1962 ◽  
Vol 39 (4) ◽  
pp. 539-546 ◽  
Author(s):  
Leif Wide ◽  
Carl Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Adult Men ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Inhibition Reaction

ABSTRACT An immunological method to assay human pituitary luteinizing hormone (HPLH) in urine is described. It is based on the fact that HPLH crossreacts with human chorionic gonadotrophin (HCG) in an haemagglutination inhibition reaction between HCG-coated blood cells and rabbit HCG-antisera. During the menstrual cycle the excretion of HPLH reached a peak of 200–400 U per liter at the time of ovulation. In the urine of post-menopausal women the concentration of HPLH was between 100 and 400 U per liter. In the urine of adult men the concentration of HPLH was between 50 and 160 U per liter.

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