scholarly journals Identification of molecular markers associated with genic male sterility in tetraploid cotton (Gossypium hirsutum L.) through bulk segregant analysis using a cotton SNP 63K array

2018 ◽  
Vol 54 (No. 4) ◽  
pp. 154-160 ◽  
Author(s):  
Dharmalingam Raja ◽  
Marappan Saravana Kumar ◽  
Ponnuswamy Renuka Devi ◽  
Sankaran Loganathan ◽  
Kamalasekharan Ramya ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Male Sterility ◽  
Seed Production ◽  
Bulk Segregant Analysis ◽  
Snp Markers ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Gossypium Hirsutum L ◽  
Cotton Hybrid ◽  
Short Time

Genic male sterility (GMS) is one of the most important economic traits for cotton (Gossypium hirsutum L.) hybrid seed production. The GMS trait conferred by two recessive alleles ms5 and ms6 in homozygous constitution is widely used for cotton hybrid seed production in India. Identification of molecular markers closely linked to the ms5 and ms6 alleles would be useful in effective transferring in a short time male-sterility genes into cultivars or elite lines using marker-assisted backcrossing. Here, we describe a quick method to identify markers for GMS genes using bulk segregant analysis (BSA) in the interspecific (G. hirsutum × G. hirsutum) biparental population. The parents and bulks were genotyped with a cotton single nucleotide polymorphism (SNP) 63K array that contains 63 058 SNP markers including 45 104 intraspecific and 17 954 interspecific SNP markers. Four SNP markers were found to be linked with the Ms5 and Ms6 genes. The markers i23493Gh and i46470Gh linked with the Ms5 gene, and other two markers i08605Gh and i08573Gh linked with the Ms6 gene are located on chromosome 12 and 26, respectively. A simple and cost effective tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR) assay was optimized for screening a large number of breeding samples with the identified SNP markers in a short time. The molecular markers developed in this study will facilitate the marker-assisted selection (MAS) and accelerate the development of new GMS lines to use in cotton hybrid seed production.    

scholarly journals Molecular regulation ofZmMs7required for maize male fertility and development of a dominant male-sterility system in multiple species

2020 ◽  
Vol 117 (38) ◽  
pp. 23499-23509 ◽  
Author(s):  
Xueli An ◽  
Biao Ma ◽  
Meijuan Duan ◽  
Zhenying Dong ◽  
Ruogu Liu ◽  
...  
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Target Genes ◽  
Hybrid Seed ◽  
Molecular Regulation ◽  
Dominant Male ◽  
Hybrid Seed Production ◽  
Maize Breeding ◽  
Pollen Exine ◽  
Multiple Species

Understanding the molecular basis of male sterility and developing practical male-sterility systems are essential for heterosis utilization and commercial hybrid seed production in crops. Here, we report molecular regulation by genic male-sterility genemaize male sterility 7(ZmMs7) and its application for developing a dominant male-sterility system in multiple species.ZmMs7is specifically expressed in maize anthers, encodes a plant homeodomain (PHD) finger protein that functions as a transcriptional activator, and plays a key role in tapetal development and pollen exine formation. ZmMs7 can interact with maize nuclear factor Y (NF-Y) subunits to form ZmMs7-NF-YA6-YB2-YC9/12/15 protein complexes that activate target genes by directly binding to CCAAT box in their promoter regions. Premature expression ofZmMs7in maize by an anther-specific promoterp5126results in dominant and complete male sterility but normal vegetative growth and female fertility. Early expression ofZmMs7downstream genes induced by prematurely expressed ZmMs7 leads to abnormal tapetal development and pollen exine formation inp5126-ZmMs7maize lines. Thep5126-ZmMs7transgenic rice andArabidopsisplants display similar dominant male sterility. Meanwhile, themCherrygene coupled withp5126-ZmMs7facilitates the sorting of dominant sterility seeds based on fluorescent selection. In addition, both thems7-6007recessive male-sterility line andp5126-ZmMs7Mdominant male-sterility line are highly stable under different genetic germplasms and thus applicable for hybrid maize breeding. Together, our work provides insight into the mechanisms of anther and pollen development and a promising technology for hybrid seed production in crops.

Research and Utilization of Chemical Inducing Male Sterility for Hybrid Seed Production in Canola (Brassica napus)

2010 ◽  
Vol 9 (1) ◽  
pp. i
Author(s):  
Chun-yun GUAN ◽  
Guo-huai WANG ◽  
She-yuan CHEN ◽  
Xun LI ◽  
Zhong-song LIU ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Seed Production ◽  
Hybrid Seed ◽  
Hybrid Seed Production

scholarly journals CHARACTERIZATION OF A MALE-STERILE SYSTEM WITH A CLOSELY LINKED SEEDLING MARKER TO FACILITATE F1 HYBRID TOMATO SEED PRODUCTION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1168e-1168 ◽  
Author(s):  
Edward C. Tigchelaar
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Marker Gene ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Chromosome 2 ◽  
Male Sterile ◽  
Tomato Seed ◽  
F1 Hybrid

The coupling phase linkages have been synthesized between the gene aw (without anthocyanin) and the male sterile gene ms15 (and its alleles ms26, ms47, and an Israeli source of male sterility). Less than 2 map units separate aw and ms15 on chromosome 2, providing a convenient seedling marker gene to rapidly identify male sterility for both inbred development and hybrid seed production. The seedling marker also provides a convenient marker to rapidly assess hybrid seed purity. Unique features of each of the alleles involved in male sterility and their use in inbred and hybrid development will be described.

scholarly journals Role of Male Sterility in Vegetable Hybrid Seed Production

2017 ◽  
Vol 6 (7) ◽  
pp. 134-141 ◽  
Author(s):  
Mopidevi M. Nagaraju ◽  
T. Thomson ◽  
G. Koteswara Rao ◽  
M. Siva
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Hybrid Seed ◽  
Hybrid Seed Production

scholarly journals A novel male sterility-fertility restoration system in plants for hybrid seed production

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Surendra Pratap Singh ◽  
Sudhir P. Singh ◽  
Tripti Pandey ◽  
Ram Rakshpal Singh ◽  
Samir V. Sawant
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Fertility Restoration ◽  
Hybrid Seed ◽  
Hybrid Seed Production
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