Protocol for Mouse IP Anesthesia (Ketamine-Xylazine) v1

Mapping Intimacies ◽

10.17504/protocols.io.bvxtn7nn ◽

2021 ◽

Author(s):

Haowei Li ◽

Markus Holzl ◽

Mohsen Khosravi-Maharlooei ◽

Nichole Danzl ◽

Austin Chen ◽

...

Keyword(s):

Minimally Invasive ◽

Open Abdomen ◽

Kidney Capsule

This protocol details the constitution of our anesthesia cocktail that we use for sedating our mice when performing surgeries. The surgeries we typically perform are thymectomies and implantation of material under the kidney capsule (through open abdomen or minimally invasive approaches).

Download Full-text

Mouse Surgery – Transplantation of human thymus into the kidney capsule of NSG mice v1

10.17504/protocols.io.bvuen6te ◽

2021 ◽

Author(s):

Austin Chen ◽

Mohsen Khosravi-Maharlooei ◽

Markus Holzl ◽

Megan Sykes

Keyword(s):

Minimally Invasive ◽

Minimally Invasive Approach ◽

Invasive Approach ◽

Kidney Capsule ◽

Human Thymus ◽

Nsg Mice ◽

Abdominal Approach ◽

Dorsal Aspect ◽

Thymus Tissue

This protocol details our minimally invasive approach for implanting human thymus tissue under the kidney capsule of NSG mice. In contrast to our open abdominal approach, this approach is from the dorsal aspect of the mouse and requires only two interrupted sutures and 1-2 staples to close the incision. The technique can be applied to other strains of mice, though we have found the NSG kidney capsule to be more delicate, and thus more challenging to manipulate during surgery.

Download Full-text

Considerations in Dysphagia Management Following Esophagectomy

Perspectives of the ASHA Special Interest Groups ◽

10.1044/persp1.sig13.169 ◽

2016 ◽

Vol 1 (13) ◽

pp. 169-176

Author(s):

Lisa M. Evangelista ◽

James L. Coyle

Keyword(s):

Esophageal Cancer ◽

Minimally Invasive ◽

Minimally Invasive Surgical Procedures ◽

Esophageal Resection ◽

Minimally Invasive Surgical ◽

Operative Complications ◽

Swallowing Difficulties ◽

Epidemiological Impact ◽

Invasive Techniques ◽

Management Of Dysphagia

Esophageal cancer is the sixth leading cause of death from cancer worldwide. Esophageal resection is the mainstay treatment for cancers of the esophagus. While curative, surgical resection may result in swallowing difficulties that require intervention from speech-language pathologists (SLPs). Minimally invasive surgical procedures for esophageal resection have aimed to reduce morbidity and mortality associated with more invasive techniques. Both intra-operative and post-operative complications, regardless of the surgical approach, can result in dysphagia. This article will review the epidemiological impact of esophageal cancers, operative complications resulting in dysphagia, and clinical assessment and management of dysphagia pertinent to esophageal resection.

Download Full-text