The effects of meta-Topolin and benzyladenine on in vitro organogenesis from epicotyl cuttings of Troyer citrange (Citrus sinensis[L.] Osbeck ×Poncirus trifoliata[L.] Raf.)

2017 ◽  
pp. 185-192 ◽  
Author(s):  
B. Chiancone ◽  
L. Martorana ◽  
V. Gianguzzi ◽  
M.A. Germanà
2009 ◽  
pp. 289-292 ◽  
Author(s):  
T.-T. Tzatzani ◽  
K. Dimassi-Theriou ◽  
I. Therios ◽  
A. Molassiotis

2011 ◽  
Vol 33 (1) ◽  
pp. 311-315 ◽  
Author(s):  
Luzia Yuriko Miyata ◽  
Francisco de Assis Alves Mourão Filho ◽  
João Alexio Scarpare Filho ◽  
Flávia Zambon ◽  
Meire Menezes Bassan ◽  
...  

Transformação genética é considerada uma importante ferramenta auxiliar no melhoramento genético de plantas cítricas. Entretanto, a eficiência de transformação pode variar em função de diversos fatores, incluindo a própria construção gênica utilizada. Este trabalho buscou avaliar a eficiência de transformação genética de plantas de citrange 'Carrizo' [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] com duas construções gênicas diferentes contendo o gene uidA (GUS) sob o controle dos promotores Arabidopsis thaliana phloem protein 2 (AtPhP2) e Arabidopsis thaliana sucrose transporter 2 (AtSuT2). Segmentos de epicótilo de plântulas germinadas in vitro foram utilizados como explantes. O gene nptII, que confere resistência ao antibiótico canamicina, foi utilizado nas construções gênicas como agente de seleção para regeneração de plantas transgênicas. O ensaio histoquímico com X-GLUC foi realizado em todas as brotações regeneradas para verificar a expressão do gene uidA. Dos 4.790 segmentos de epicótilo utilizados, registrou-se a regeneração de 366 brotações com reação positiva no ensaio histoquímico, as quais foram enxertadas em porta-enxertos cultivados in vitro. Cinco dessas brotações, de cada construção gênica, foram selecionadas para análise da PCR, com primers específicos para amplificação da sequência do gene uidA. A inserção do transgene foi confirmada por PCR em todas as brotações selecionadas. A eficiência de transformação e o número de brotos escapes, avaliada pelo teste histoquímico, variaram em função das construções gênicas utilizadas.


HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 421D-421
Author(s):  
M.M. Khattab ◽  
A.A. Elezaby ◽  
S. ElOraby ◽  
A.M. Hassan

This investigation was carried out on 13-year-old Valencia orange trees [Citrus sinensis (L.) Osbeck] budded on five different rootstocks. Heat unit accumulation (temperature above 12.5 °C) for fruits worked on the various rootstocks were calculated from full bloom to maturity stage. Valencia fruits on Troyer citrange (C. sinensis × Poncirus trifoliata) and Carrizo citrange (C. sinensis × Poncirus trifoliata) rootstocks matured earlier when compared to those growing on Cleopatra mandarin (C. reticulata Blanco), Volkamer lemon (C. volkameriana Ten. and Pasq.), and sour orange (C. aurantium) rootstocks. The results showed that the Valencia fruits, regardless of rootstock, could be stored for different periods under different conditions. However, in order to avoid degradation in fruit quality, storing periods should not exceed 21, 60, and 120 days under room conditions (25 °C and RH 25% to 35%), 4 °C (RH 80% to 85%), and 8 °C (RH 80% to 85%); respectively.


1999 ◽  
Vol 124 (2) ◽  
pp. 152-157 ◽  
Author(s):  
Fabio De Pasquale ◽  
Salvatore Giuffrida ◽  
Francesco Carimi

Minigrafting was used for rescue of tissue culture regenerants of the following four species of Citrus: sour orange (C. aurantium L. `AA CNR 31'), sweet orange [C. sinensis (L.) Osb. `Valencia Late'], lemon [C. limon (L.) Burm. `Femminello Comune'] and mandarin (C. deliciosa Tenore `Tardivo di Ciaculli'). The grafting was carried out with different scion types including shoots, roots, inverted roots and somatic embryos. This material was obtained in vitro from embryogenic style-derived callus. Seedlings of open-pollinated sour orange (C. aurantium L.), Cleopatra mandarin (C. reshni Hort. ex Tan.) and `Troyer' citrange [C. sinensis Osb. × Poncirus trifoliata (L.) Raf.] were used as rootstocks. Minigrafting of shoots, roots, inverted roots and embryos regenerated in vitro allowed successful rescue of these four species. Percentages of successful minigrafts ranged from 100% (shoots) to 2.5% (inverted roots). The probability of successful graft unions increased with the age of the rootstock. The final mean canopy leaf area (120 days after grafting) ranged from 5.2 cm2 (`Tardivo di Ciaculli' mandarin grafted on 6-month-old Cleopatra mandarin) to 157.9 cm2 (`Valencia Late' sweet orange grafted on 18-month-old Cleopatra mandarin). In this work we examined some of the variables which influenced minigrafting and we determined the efficacy of this method for rescue of in vitro regenerants of Citrus. This method is also suggested as a technique to produce a high percentage of viable plants from in vitro regenerants difficult to root.


HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1116-1119 ◽  
Author(s):  
S. Verdejo-Lucas ◽  
F.J. Sorribas ◽  
J.B. Forner ◽  
A. Alcaide

The response of 22 citrus hybrid rootstocks to a Mediterranean biotype of Tylenchulus semipenetrans Cobb was determined in a greenhouse test. Five of the 11 rootstocks with Poncirus trifoliata (L.) Raf in their parentage did not support nematode reproduction. However, rootstocks with Troyer citrange [Citrus sinensis (L.) Osbeck × P. trifoliata (L.) Raf] in their parentage were susceptible to the nematode.


2001 ◽  
Vol 126 (5) ◽  
pp. 599-605 ◽  
Author(s):  
Brandon R. Hockema ◽  
Ed Etxeberria

The nature of sink strength in orange fruit and changes occurring during drought stress were investigated. Potted trees of `Hamlin' orange [Citrus sinensis (L.) Osbeck] grafted on Troyer citrange [Citrus sinensis × Poncirus trifoliata (L.) Raf.] were irrigated using a microsprinkler system creating either well-watered or water-stressed conditions, as determined by stem water potentials. Fruit were harvested every other week from trees of both well-watered and drought-stressed treatments during the final stage of fruit development when sugars accumulate rapidly. Fruit quality indices and activities of sucrose synthase (SuSy), invertase, sucrose-P synthase, sucrose-P phosphatase, V-ATPase, and V-PPase were measured. Acids and soluble sugar concentrations were elevated in drought-stressed fruit, whereas juice pH decreased in those same fruit. Results indicate that increased sink strength in fruit from stressed trees was accompanied by an increase in SuSy activity and lowered juice pH. The remaining enzymes examined in this experiment showed no changes in activity between control and treated fruit, as was the case for plasmalemma and tonoplast sucrose carriers. Based on the present data, we conclude that SuSy and vacuolar pH are the predominant factors controlling photoassimilate accumulation in orange fruit under enhanced sink conditions brought about by imposition of a mild drought stress.


Sign in / Sign up

Export Citation Format

Share Document