Absorption and Distribution of Calcium (45Ca) Applied to the Surface of Orange (Citrus sinensis) Fruits at Different Developmental Stages

In this study, the rate of absorption and transport of calcium (Ca) in orange fruits (Citrus sinensis cv. Fukumoto) after surface treatment with 45Ca was evaluated by supplying treatments at different dates between fruit set and mid-phase II of growth (i.e., 30, 44, 66 and 99 days after full bloom, DAFB). 45Ca absorption was always detected, but 45Ca application at the fruit set was associated with the highest rates of Ca transport into the pulp (39%) compared to the other treatments (17–19%). Scanning electron microscopy SEM) images of the fruit surface showed the occurrence of stomata along with the entire rind at all the developmental stages evaluated. However, from the beginning of stage I, stomata began to collapse and develop plugs, and this became frequent as the fruit age increased. From 44 DAFB, oil gland density increased, and Ca oxalates (CaOx) were found in the fruit flavedo and albedo. Fruit Ca (not labeled) concentration increased from fruit set (30 DAFB) to 99 DAFB, although oil gland formation and rind growth and differentiation likely hindered Ca transport to internal fruit tissues (pulp). The total Ca concentration in the pedicel was always higher than that in the fruit, with no differences between the first three treatments. The information obtained in this study may be essential for improving Ca fertilizer efficacy in citrus by spray applications.

Nutrient Management Effects on Wine Grape Tissue Nutrient Content

With limited research supporting local nutrient management decisions in North Carolina grape (Vitis vinifera) production, field studies (2015–17) were conducted to evaluate late season foliar nitrogen (N) application on leaf and petiole N concentration and yeast assimilable N (YAN) in the fruit. Foliar urea (1% v/v) was applied at different rates and application times beginning pre-and post-veraison. Compared to soil applied N, late season foliar N substantially enhanced petiole N and grape YAN. Smaller split N applications were generally more effective in increasing YAN than single larger N rates. These data demonstrate the value of assessing plant N content at full bloom with petiole N analysis or remote sensing to guide foliar N management decisions. Additional field studies (2008–11) were conducted to evaluate pre-bud soil applied phosphorus (P) and potassium (K) effects on petiole P and K nutrient status. Fertilizer P and K were initially broadcast applied (0–896 kg P2O5 ha−1; 0–672 kg K2O ha−1) prior to bud-break in 2008–09 and petiole P and K at full bloom soil test P and K were monitored for three to four years after application. Soil test and petiole P and K were significantly increased with increasing P and K rates, which subsequently declined to near unfertilized levels over the sampling time depending on site and P and K rate applied. These data demonstrate the value of annually monitoring petiole P and K levels to accurately assess plant P and K status to better inform nutrient management decisions.

Humik Asit Uygulamalarının Zambak Bitkisinin Gelişimi, Bitkideki Fenolojik ve Pomolojik Gözlemler Üzerine Etkisi

In this study, a pot study was carried out to reveal the effects of direct and foliar applications of humic acid to the growing medium in addition to basic fertilization on the development and plant quality characteristics of lily plants grown in peat + perlite medium. The experiment was carried out in an unheated greenhouse during the summer, in a greenhouse environment with a shade net on it. In the experiment, in addition to the control, the humic acid doses were 3.5 lt/da from soil, 7.5 lt/da from soil, 1.75 lt/da from leaf, 3.75 lt/da from leaf and soil + 3.5 lt/da from leaf + 1, It was applied 3 times as 75 lt/da. In the pot experiment established in three replications according to the randomized plots trial design, 10 lily bulbs were planted in each pot and humic acid applications were made at two-week intervals following plant emergence. In order to see the effect of the applications when the plant is harvested, the length of the flower stem (mm), the thickness of the flower stem (mm), the fresh weight of the branch (mm), the number of buds (pieces), the bud length (cm), the number of leaves (pieces), Full bloom stem (mm) and vase life (days) were examined. As the doses of humic acid increased, the vegetative weight of the lily plant increased, while the effect of the applications on the investigated properties was different. With the mineral elements, hormones and plant growth regulators in humic acid, it has the feature of promoting the increase of biomass in the plant.

Digitalization and Urbanization

The business world is transforming into digitalization. To stay modest and appropriate, it has become necessary to adopt digitalization. The construction industry is entirely ready for the opportunity that will come through digitalization. Currently, we are in the finishing days of the industrial age. With this advanced revolution, the future will be reshaped in two trends: digitalization and urbanization. At the starting of the 19th century when the industrial revolution was in full bloom, a small amount of the population was living in the cities. As it has been for thousands of years, the world is still rural and agricultural.

A Study on Hair Art with the Motif of Winter Flowers

The purpose of this study is to increase the artistic value of hair art. And it is about providing basic data and expanding contributions to follow-up research. Hair art works reinterpret the inner beauty of winter flowers that bloom in full bloom in the cold winter. The research method collected prior studies and references based on winter flowers. Also, we selected four types of winter flowers and analyzed their shapes and colors. I created 4 pieces of hair art. As a result, the colorful color of work 1 increased the aesthetic effect due to its high chroma. Works 2 expresses the elegance of irregularly blooming petals on a single stalk. In work 3, warm yellow flowers are soft and cozy on a scrawny tree. The flower in work 4 is seductive navy purple. And the large and small flowers were placed stably with increased concentration. In conclusion, the possibility of creating hair art from a new perspective was confirmed by applying several colors with white wigs. In future research, we look forward to the possibility of combining hair art with nature motifs and re-lighting it with formative art to satisfy its value.

Metabolite and Transcriptome Profiling Analysis Revealed That Melatonin Positively Regulates Floral Scent Production in Hedychium coronarium

Melatonin is a pleiotropic molecule that regulates a variety of developmental processes. Floral volatiles are important features of flowers that facilitate flower–visitor interactions by attracting pollinators, structure flower–visitor communities, and play defensive roles against plant and flower antagonists. Aside from their role in plants, floral volatiles are an essential ingredient in cosmetics, perfumes, pharmaceuticals, and flavorings. Herein, integrated metabolomic and transcriptomic approaches were carried out to analyze the changes triggered by melatonin exposure during the Hedychium coronarium flower development stages. Quantitative analysis of the volatiles of H. coronarium flowers revealed that volatile organic compound emission was significantly enhanced after melatonin exposure during the half bloom (HS), full bloom (FB) and fade stage (FS). Under the melatonin treatment, the emission of volatile contents was highest during the full bloom stage of the flower. Variable importance in projection (VIP) analysis and partial least-squares discriminant analysis (PLS-DA) identified 15 volatile compounds with VIP > 1 that were prominently altered by the melatonin treatments. According to the transcriptome sequencing data of the HS, FB, and FS of the flowers, 1,372, 1,510, and 1,488 differentially expressed genes were identified between CK-HS and 100MT-HS, CK-FB and 100MT-FB, and CK-FS and 100MT-FS, respectively. Among the significant differentially expressed genes (DEGs), 76 were significantly upregulated and directly involved in the floral scent biosynthesis process. In addition, certain volatile organic compounds were substantially linked with various DEGs after combining the metabolome and transcriptome datasets. Moreover, some transcription factors, such as MYB and bHLH, were also significantly upregulated in the comparison, which might be related to the floral aroma mechanism. Our results suggested that melatonin increased floral aroma production in H. coronarium flowers by modifying the expression level of genes involved in the floral scent biosynthesis pathway. These findings serve as a foundation for future research into the molecular mechanisms underlying the dynamic changes in volatile contents induced by melatonin treatment in H. coronarium.

Evaluation of Phenological and Agronomical Traits of Different Almond Grafting Combinations under Testing in Central Italy

In the new introducing almond areas, it is necessary to test the more promising almond cultivar and rootstock combinations able to guarantee the best agronomic performances according to the specific pedoclimatic conditions. With this aim, two almond trials have been established in an experimental farm located in the Latium region (Italy). The first trial (A) focused on the phenological, and agronomical influences induced by the clonal rootstock ‘GF677’ on the grafted cultivars ‘Tuono’, ‘Supernova’ and ‘Genco’, in comparison to those induced by peach seedling rootstocks, in order to identify the best grafting combination for developing “high density” plantings in this new growing area. The second trial (B) tested the phenological and agronomical influences induced by three different clonal rootstocks (‘GF677’, ‘Rootpac® 20’ and ‘Rootpac® R’), on the Spanish cultivar ‘Guara’ to identify suitable dwarfing rootstocks for “super high density” plantings in the same environment. Flowering and ripening calendars of the trial A highlighted as the medium-late flowering cultivars ‘Genco’, ‘Supernova’ and ‘Tuono’ could be subject to moderate risk of cold damages. The clonal rootstock ‘GF677’ seems to anticipate flowering and vegetative bud break by a few days in ‘Tuono’ when compared to the same cultivar grafted on peach seedling rootstocks. Furthermore, the yield per plant was always higher in plants grafted on ‘GF677’. The observations carried out in trial B highlighted as the flowering of cultivar ‘Guara’ were affected by the rootstock, with ‘Rootpac® 20’, which postponed its full bloom of about one week when compared to other rootstocks, whereas ‘GF677’ imposed more vigor to the cultivar than ‘Rootpac® 20’ and ‘Rootpac® R’.

PHYSICOCHEMICAL CARACTERISTICS OF UNRIPE APPLES

Apple plays an important role in human health. The unripe apples, after physiological falls and the agricultural operation "regulation of the fruit load", are not used for food. The use of agricultural organic waste is a current problem. In the presented paper was approached the possibility of valorification on these unripe fruits to obtain natural products. Were studied the unripe apples of 4 varieties: Coredana, Golden Rezistent, Rewena and Reglindis. The fruits were harvested during development at 45th, 58th, 71st, 84th and 97th days after the full bloom. It was determined physico-chemical indices in fruits extracts. During the ripening of fruits, the diameter and mass increased continuously, the moisture content does not change significantly, but the pH values of the apple pulp increased nonessential. Dry matter content and total sugar increased considerably as the fruits were growing. There are unexpected increases in dry matter around the 84th day after the full bloom. The acidity decreased during the fruit development. Sensory evaluation was also effectuated. The results obtained provide informations regarding the optimal use of the unripe apple varieties researched in order to process and obtain different products (especially natural acidifiers).

Sensitivity of Some Sweet Cherry (Prunus Avium L.) Genotypes To Late Spring Frosts During Different Phenological Stages Following Bud Burst

Sweet cherry (Prunus avium L.) is one of the economically important fruit crops worldwide. However, late spring frosts occurring in some years can significantly impact sweet cherry productivity through organ and tissue destruction caused by frost damage, and very little is known about frost tolerance or susceptibility of new cultivars. Differential thermal analysis (DTA) was, therefore, used to examine the exothermic characteristics (temperatures at which 50% of the flower buds were killed - mLTE values) of the flower buds belonging to members of the genus Prunus - 6 Prunus avium cultivars ('Noir de Guben', 'Bigarreau Gaucher', 'Merton Late', 'Merton Bigarreau', 'Van' and wild genotype). In the study, mLTE values of flowers of six cherry cultivars were determined at different floral bud developmental stages such as side green, green tip, open cluster, first white and full bloom under laboratory-based freeze assays for consecutive two years. The mLTE values of flower buds changed according to both different floral bud developmental stages and sweet cherry cultivars. In our findings, the mLTE values of flower buds in all cultivars generally occurred at higher temperatures in the open cluster stage, whereas the mLTE values of the flower buds in the first white stage occurred at lower temperatures, and therefore, these results are not only controversial in terms of previous frost tolerance studies, but also the first findings to be reported in literature. Considering the two-year average, the temperatures causing mLTE values for flower buds was -1.58 to -3.74°C at the side green stage, -0.94 to -3.51°C at the green tip stage, -0.41 to -1.96°C at the open cluster stage, -2.30 to -11.52°C at the first white stage and -2.37 to -9.80°C at the full bloom stage in the range of six cultivars. In laboratory-based freezing experiments, the 'Van' cultivar were least affected by low temperatures, followed by 'Bigarreau Gaucher' cultivar and wild genotype. 'Merton Late' cultivar, on the contrary, was the most sensitive cultivar to low temperatures, followed by 'Noir de Guben' and 'Merton Bigarreau'. These results can be valuable in predicting possible frost damage at different developmental stages of the flower buds in sweet cherry.

Comparative fungal diversity and dynamics in plant compartments at different developmental stages under root-zone restricted grapevines

Background The root-zone restriction cultivation technique is used to achieve superior fruit quality at the cost of limited vegetative and enhanced reproductive development of grapevines. Fungal interactions and diversity in grapevines are well established; however, our knowledge about fungal diversity under the root-zone restriction technique is still unexplored. To provide insights into the role of mycobiota in the regulation of growth and fruit quality of grapevine under root-zone restriction, DNA from rhizosphere and plant compartments, including white roots (new roots), leaves, flowers, and berries of root-zone restricted (treatment) and conventionally grown plants (control), was extracted at three growth stages (full bloom, veraison, and maturity). Results Diversity analysis based on the ITS1 region was performed using QIIME2. We observed that the root-zone restriction technique primarily affected the fungal communities of the soil and plant compartments at different growth stages. Interestingly, Fusarium, Ilyonectria, Cladosporium and Aspergillus spp observed in the rhizosphere overlapped with the phyllosphere at all phenological stages, having distinctive abundance in grapevine habitats. Peak richness and diversity were observed in the rhizosphere at the full bloom stage of control plants, white roots at the veraison stage of treatment, leaves at the maturity stage of treatment, flowers at the full bloom stage and berries at the veraison stage of control plants. Except for white roots, the diversity of soil and plant compartments of treated plants tended to increase until maturity. At the maturity stage of the treated and control plants, the abundance of Aspergillus spp. was 25.99 and 29.48%, respectively. Moreover, the total soluble sugar content of berries was 19.03 obrix and 16 obrix in treated and control plants, respectively, at the maturity stage. Conclusions This is the first elucidative study targeting the fungal diversity of conventional and root-restricted cultivation techniques in a single vineyard. Species richness and diversity are affected by stressful cultivation known as root zone restriction. There is an association between the abundance of Aspergillus spp. and fruit quality because despite causing stress to the grapevine, superior quality of fruit is retrieved in root-zone restricted plants.