scholarly journals MEK inhibitor PD0325901 and vitamin C synergistically induce hypomethylation of mouse embryonic stem cells

Oncotarget ◽  
2016 ◽  
Vol 7 (26) ◽  
pp. 39730-39739 ◽  
Author(s):  
Cuiping Li ◽  
Baodong Liu ◽  
Shangwei Zhong ◽  
Hailin Wang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Vitamin C ◽  
Embryonic Stem ◽  
Mek Inhibitor ◽  
Mouse Embryonic Stem Cells

Melatonin‐mediated upregulation of GLUT1 blocks exit from pluripotency by increasing the uptake of oxidized vitamin C in mouse embryonic stem cells

2017 ◽  
Vol 31 (4) ◽  
pp. 1731-1743 ◽  
Author(s):  
Haibo Wu ◽  
Chao Song ◽  
Jingcheng Zhang ◽  
Jiamin Zhao ◽  
Beibei Fu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Vitamin C ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Kevin T. Ebata ◽  
Kathryn Mesh ◽  
Shichong Liu ◽  
Misha Bilenky ◽  
Alexander Fekete ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Vitamin C ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Vitamin C induces a pluripotent state in mouse embryonic stem cells by modulating microRNA expression

FEBS Journal ◽  
2015 ◽  
Vol 282 (4) ◽  
pp. 685-699 ◽  
Author(s):  
Yuan Gao ◽  
Zhuo Han ◽  
Qian Li ◽  
Yongyan Wu ◽  
Xiaoyan Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Vitamin C ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Microrna Expression ◽  
Pluripotent State

scholarly journals The distinct effects of MEK and GSK3 inhibition upon the methylome and transcriptome of mouse embryonic stem cells

2021 ◽  
Author(s):  
Julia Spindel ◽  
Christel Krueger ◽  
Felix Krueger ◽  
Evangelia K Papachristou ◽  
Kamal Kishore ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
De Novo ◽  
Embryonic Stem ◽  
Mek Inhibitor ◽  
Mouse Embryonic Stem Cells ◽  
Leukaemia Inhibitory Factor ◽  
Differentiation Markers ◽  
Chromatin Signature

Mouse embryonic stem cells (mESCs) were first cultured in vitro in serum-containing medium with leukaemia inhibitory factor, in which they exhibit heterogeneous expression of both pluripotency and some early differentiation markers. Over the last decade, however, it has become commonplace to grow mESCs with inhibitors of MEK and GSK3 signalling, which together elicit a more homogeneously 'naive' state of pluripotency. Whilst 2i/L-cultured mESCs have been shown to be globally hypomethylated, a comprehensive understanding of the distinct effects of these signalling inhibitors upon the DNA methylome is still lacking. Here we carried out whole genome bisulphite and RNA sequencing of mESCs grown with MEK or GSK3 inhibition alone, including different time points and concentrations of MEK inhibitor treatment. This demonstrated that MEK inhibition causes a dose-dependent impairment of maintenance methylation via loss of UHRF1 protein, as well as rapid impairment of de novo methylation. In contrast, GSK3 inhibition triggers impairment of de novo methylation alone, and consequent hypomethylation is enriched at enhancers with a 2i/L-specific chromatin signature and coincides with upregulation of nearby genes. Our study provides detailed insights into the epigenetic and transcriptional impacts of inhibiting MEK or GSK3 signalling in mouse pluripotent cells.

Melatonin affects hypoxia-stressed cardiomyocyte differentiation of mouse embryonic stem cells

2020 ◽  
Author(s):  
Eui-Bae Jeung ◽  
Jae-Hwan Lee ◽  
Park Seon Mi ◽  
Sunhwa Jeong ◽  
Yeong-Min Yoo
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cardiomyocyte Differentiation
Sign in / Sign up

Export Citation Format

Share Document