scholarly journals Microsatellite marker analysis of macedonian grapevines (Vitis vinifera L.) compared to Bulgarian and Greek cultivars

OENO One ◽  
2009 ◽  
Vol 43 (1) ◽  
pp. 29
Author(s):  
Natasa Stajner ◽  
Elizabeta Angelova ◽  
Zvonimir Bozinovic ◽  
Mihail Petkov ◽  
Branka Javornik
Keyword(s):  
Vitis Vinifera ◽  
Microsatellite Marker ◽  
Microsatellite Loci ◽  
Genetic Background ◽  
Genetic Characterization ◽  
Molecular Investigation ◽  
Balkan Region ◽  
Shared Alleles ◽  
Microsatellite Marker Analysis

<p style="text-align: justify;"><strong>Aims</strong>: Eleven Macedonian grapevine accessions were genotyped by microsatellite profiling at 9 microsatellite loci, in order to identify Macedonian cultivars and to evaluate the relationships among them. The comparison with grapevine cultivars from two neighbouring countries was also performed.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Clustering analyses based on the proportion of shared alleles resulted in two clusters containing all accessions except cultivar « Vranec », which was distant from the others. Comparison of genotyping results of Macedonian accessions with 76 Bulgarian and 298 Greek accessions revealed no identical genotypes. In the dendogram, Macedonian accessions are dispersed among Greek and Bulgarian grapevines, suggesting a common genetic background. Additionaly, the synonyms « Smederevka » = « Dimyat » = « Zoumiatiko » and « Belo Zimsko » = « Karatsova Naousis » were also evaluated.</p><p style="text-align: justify;"><strong>Conclusions</strong>: Clustering analyses showed that authentic Macedonian cultivars are distant from two widespread cultivars « Vranec » and « Smederevka ». Comparison of Macedonian cultivars with their synonyms from Greece and Bulgaria revealed differences in allelic profiles at some loci, but further analyses are needed to confirm their unique allelic profiles.</p><p style="text-align: justify;"><strong>Significance and impact of study</strong>: This work is a first step towards the genetic characterization of Macedonian grapevine germplasm, thus contributing to the molecular investigation of grapevine germplasm within the Balkan region.</p>

Genetic characterization of local goats of Karnataka by microsatellite marker analysis

2018 ◽  
Author(s):  
R. Jayashree ◽  
M. R. Jayashankar ◽  
C. S. Nagaraja ◽  
Isloor Shrikrishna ◽  
K. Satyanarayana
Keyword(s):  
Microsatellite Marker ◽  
Genomic Dna ◽  
Genetic Characterization ◽  
Expected Heterozygosity ◽  
Marker Analysis ◽  
Goat Population ◽  
Pcr Products ◽  
The Mean ◽  
Microsatellite Marker Analysis

The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka.

Characterization of the 17p amplicon in human sarcomas — microsatellite marker analysis

1997 ◽  
Vol 98 (2) ◽  
pp. 169
Author(s):  
M. Wolf ◽  
M. Tarkkanen ◽  
L.A. Aaltonen ◽  
A. Forus ◽  
M. Larramendy ◽  
...  
Keyword(s):  
Microsatellite Marker ◽  
Marker Analysis ◽  
Microsatellite Marker Analysis

scholarly journals Characterization of the 17p amplicon in human sarcomas: Microsatellite marker analysis

1999 ◽  
Vol 82 (3) ◽  
pp. 329-333 ◽  
Author(s):  
Maija Wolf ◽  
Maija Tarkkanen ◽  
Theo Hulsebos ◽  
Marcelo L. Larramendy ◽  
Anne Forus ◽  
...  
Keyword(s):  
Microsatellite Marker ◽  
Marker Analysis ◽  
Microsatellite Marker Analysis

scholarly journals Genetic characterization of 18 novel microsatellite loci in northern pike (Esox lucius L.)

2010 ◽  
Vol 34 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Jun Wang ◽  
Chenghui Wang ◽  
Long Qian ◽  
Yuqing Ma ◽  
Xinxin Yang ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Genetic Characterization ◽  
Northern Pike ◽  
Esox Lucius ◽  
Pike Esox Lucius

Molecular and genetic characterization of the allelic variants of Du215, Du281, Du323, and Du47G microsatellite loci in parthenogenetic lizard Darevskia armeniaca (Lacertidae)

2017 ◽  
Vol 53 (4) ◽  
pp. 472-482 ◽  
Author(s):  
A. E. Girnyk ◽  
A. A. Vergun ◽  
A. V. Omelchenko ◽  
V. G. Petrosyan ◽  
V. I. Korchagin ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Genetic Characterization ◽  
Allelic Variants

GENETIC CHARACTERIZATION OF VITIS VINIFERA ACCESSIONS CULTIVATED IN SICILY (ITALY)

2009 ◽  
pp. 177-182 ◽  
Author(s):  
C. Maitti ◽  
L. Andreani ◽  
F. Geuna ◽  
L. Brancadoro ◽  
A. Scienza
Keyword(s):  
Vitis Vinifera ◽  
Genetic Characterization

scholarly journals Genetic characterization of Albanian grapevine cultivars by microsatellite markers

OENO One ◽  
2005 ◽  
Vol 39 (3) ◽  
pp. 109
Author(s):  
Emmanuel D. Ladoukakis ◽  
François Lefort ◽  
Petraq Sotiri ◽  
Arjola Bacu ◽  
Efigjeni Kongjika ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Genetic Characterization ◽  
Allelic Profile ◽  
Genetic Profiling ◽  
Nuclear Microsatellite ◽  
Genetic Profiles

<p style="text-align: justify;">A recently restored ampelographic collection of Albanian grapevine accessions has been submitted to genetic profiling with eleven nuclear microsatellite markers, widely used in other studies. Microsatellite profiling resulted in 28 single profiles for 29 accessions. Two cultivars, Shesh I bardhë and Pucalla, were found to be synonyms. Genetic profiles of Albanian cultivars were compared at 8 microsatellite loci to 29 most commonly cultivated Greek cultivars. Albanian cultivars were found to be more closely related to Greek cultivars from Peloponnese. One Greek cultivar named Dempina was found to be genetically close to two Albanian cultivars Debina teki and Debina kala, which are homonyms Another cultivar, known as Toska or Sinambel displayed a tri-allelic profile at 5 loci over 10 analysed loci. Such a high number of tri-allelic loci found in one individual favours the hypothesis of triploidy but the chimerism hypothesis cannot be excluded without further work.</p>

Pathology and Molecular Characterization of Porcine Sapelovirus in Indian Pigs

2021 ◽  
Author(s):  
Shailesh Kumar Patel ◽  
Mamta Pathak ◽  
Alok Singh ◽  
Aditya Agrawal ◽  
Jigyasa Rana ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Histopathological Examination ◽  
Rna Virus ◽  
Experimental Studies ◽  
Morbidity Rate ◽  
High Morbidity ◽  
High Morbidity Rate ◽  
Molecular Investigation ◽  
Porcine Sapelovirus

Background: The porcine sapelovirus (PSV) is a small, non-enveloped, single-stranded, positive-sense, RNA virus of the family Picornaviridae. The PSV infections in pigs have been found associated with diarrhoea, polioencephalomyelitis, pneumonia and reproductive disorders with a high morbidity rate. Despite of its economical importance very few studies are available on the pathology of PSV. The present study was conducted with the aim to investigate the PSV infection and associated pathology in Indian pigs. Methods: Tissue samples along with intestinal content were collected from a total of 78 necropsied cases for histopathological examination and molecular investigation during April 2019 to August 2020. The amplification of 5' UTR region of PSV was carried out via RT-PCR and confirmed by sequencing. The Genetic characterization of Indian isolate of the PSV was done on the basis of viral 5' UTR gene. Result: A total of eight out of 78 cases were found positive for the PSV. Catarrhal and haemorrhagic enteritis, thickening and clouding of brain meninges along with congestion of brain and pneumonia was observed as common gross lesions. Microscopic lesions included perivascular cuffing, focal gliosis, neuronophagia, congestion of meningeal and cerebral vessels, interstitial pneumonia, inflammatory changes in the intestinal mucosa and sloughing of villi. The genetic characterization revealed maximum identity of 96.89% with PSV-1 strain PSV-46-V (LC508233) and PSV-1 strain PSV-26-B (LC508232) of Zambia. This study reported the pathological and molecular investigation of PSV from Indian pigs. Further explorative surveillance along with experimental studies in suitable animal model and cell lines are highly warranted for better understanding of PSV pathology in Indian pigs.

scholarly journals Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers

2012 ◽  
Vol 99 (2) ◽  
Author(s):  
Lidija Tomić ◽  
Nataša Štajner ◽  
Tatjana Jovanović Cvetković ◽  
Miljan Cvetković ◽  
Branka Javornik
Keyword(s):  
Vitis Vinifera ◽  
Genetic Characterization ◽  
Aflp Markers

scholarly journals Genetic Characterization of Six Stocks ofLitopenaeus vannameiUsed in Cuba for Aquaculture by Means of Microsatellite Loci

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Anna Pérez-Beloborodova ◽  
Adriana Artiles-Valor ◽  
Lourdes Pérez-Jar ◽  
Damir Hernández-Martínez ◽  
Missael Guerra-Aznay ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Microsatellite Loci ◽  
First Generation ◽  
Second Generation ◽  
Genetic Characterization ◽  
Parental Population ◽  
Allelic Variants ◽  
The Third ◽  
Private Alleles

Four microsatellite loci were used to achieve genetic characterization of six stocks fromLitopenaeus vannameiused for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.

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