scholarly journals Isolasidan Karakterisasi Morfologi Aktinomiset Indigenus Asal Tanah Gambut

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Eka Astuty

Aktinomiset merupakan kelompok mikroorganisme yang tersebar luas di lingkungan darat, air tawar dan laut. Aktinomiset memainkan peran penting dalam dekomposisi bahan organik dan dengan demikian mengisi pasokan nutrisi dalam tanah.Penelitian ini bertujuan untuk mengisolasi dan karakterisasi morfologi aktinomiset indigenus asal tanah gambut. Isolasi aktinomiset dari contoh tanah gambut yang memiliki karakteristik pH 4.7, rasio C/N 38 dan nilai KTK 70.5 asal Tanjung Jabung Barat, Jambi.Isolat yang diperoleh dimurnikan dan diremajakan kembali pada media Yeast Malt Agar (YMA). Isolat aktinomiset yang telah murni ditumbuhkan pada tiga jenis media pertumbuhan yaitu Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), dan Oatmeal Agar (OM) selama ±10 hari pada suhu ruang. Selanjutnya, dilakukan pengelompokan isolat aktinomiset berdasarkan warna koloninya. Terdapat 80 koloni aktinomiset yang berhasil diisolasi dari contoh tanah gambut, yang tumbuh pada media agar-agar HV dan dimurnikan di media YMA.  Hasil seleksi berdasarkan keragaman bentuk koloni dari 80 isolat diperoleh 20 isolat murni yang memiliki beragam morfologi koloni, menghasilkan miselia aerial dan mampu tumbuh baik serta bersporulasi pada umur 7-14 hari. Karakteristik morfologi ini mengindikasikan isolat-isolat tersebut adalah Streptomyces sp.

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381 ◽  
Author(s):  
K Ko ◽  
HM Ge ◽  
J Shin ◽  
DC Oh

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  

2020 ◽  
Vol 16 ◽  
Author(s):  
Nidhi Srivastava ◽  
Indira P. Sarethy

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.


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