scholarly journals Particle Bombardment of Apple Leaf Explants Influences Adventitious Shoot Formation

HortScience ◽  
1994 ◽  
Vol 29 (12) ◽  
pp. 1536-1538 ◽  
Author(s):  
P. Gercheva ◽  
R.H. Zimmerman ◽  
L.D. Owens ◽  
C. Berry ◽  
F.A. Hammerschlag
Keyword(s):  
Shoot Regeneration ◽  
Particle Bombardment ◽  
Dark Period ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Medium ◽  
Adventitious Shoot Formation ◽  
Shoot Production ◽  
And Control

Shoot regeneration from apple (Malus domestica Borkh.) leaf explants following particle bombardment at various acceleration pressures was studied. Basal leaf segments of micropropagated `Royal Gala' apple were bombarded with 1 μm gold particles, accelerated using helium pressures of 4.5, 6.2, 7.6, 9.3, or 13.8 MPa (650–2000 psi), and cultured on shoot regeneration medium consisting of N6 salts supplemented with 10 μM TDZ for 5, 10, or 20 days in darkness. Bombarded and control explants exhibited 63% to 100% shoot regeneration. With a 5-day dark period, average shoot production per explant ranged from 6.1 to 14; bombardments of 4.5 and 6.2 MPa significantly increased shoot production over the controls. With a 10-day dark period, average shoot production per explant ranged from 9.1 to 22 following bombardment at 9.3 and 6.2 MPa, respectively. Following bombardment at 6.2 MPa, 75% of the explants produced more than 20 regenerants per explant. With a 20-day dark period, average shoot production per explant ranged from 8.9 to 19 following bombardment at 13.8 MPa and following no bombardment, respectively. Shoot production per explant was significantly less than the controls following bombardments ranging from 6.2 to 13.8 MPa. Shoot production was highest per explant with particle bombardment at 6.2 MPa followed by incubation in darkness for 10 days. Chemical name used: thidiazuron (TDZ).

scholarly journals Micropropagation of Urginea maritima (L.) Baker s. str.

2014 ◽  
Vol 62 (1-2) ◽  
pp. 11-15 ◽  
Author(s):  
Anna Stojakowska
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation ◽  
Urginea Maritima

A method of micropropagation of <i>Urginea maritima</i> (L.) Baker s.str. (<i>Liliaceae</i>) by adventitious shoot formation was developed. Bulb scales and leaf fragments were used as primary and secondary explants, respectively. The most favourable for shoot regeneration were media: MS supplemented with BAP or kinetin (bulb scales) and medium C containing NAA along with BAP (leaf explants). No difficulties in rooting and adapting of plants to greenhouse conditions were observed.

scholarly journals Regenerating Plants from in Vitro Culture of Black Locust Cotyledon and Leaf Explants

HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 942-945 ◽  
Author(s):  
I. Arrillaga ◽  
S.A. Merkle
Keyword(s):  
Shoot Regeneration ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Regeneration Frequency ◽  
Adventitious Shoot Formation

A protocol to achieve efficient plant regeneration from juvenile black locust (Robinia pseudoacacia L.) explants is described. Direct adventitious shoots were induced from cotyledon explants on woody plant medium containing 22.2 μm BA and 0.4 μm 2,4-D. Shoots developed and new shoots were induced when the explants were transferred to medium without growth regulators. The effect of dark incubation on shoot regeneration from cotyledons indicated that 15 days of darkness resulted in a high regeneration frequency (91.7%). Adventitious shoot formation also was induced from sections of in vitro-derived leaves cultured in darkness on Murashige and Skoog medium supplemented with 4.4 μm BA and 24.6 μm IBA. A shoot regeneration frequency of 89% was obtained when explants were subcultured on a medium containing 4.4 μm BA and 0.5 μm IAA. Shoots were rooted on Schenk and Hildebrandt medium with or without IBA. Plantlets were acclimatized and grown in the greenhouse. Chemical names used: N -(phenylmethyl)-1H -purin-6-amine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA).

scholarly journals Development of an in vitro Callus Induction Protocol and Shoot Proliferation for Selected Jatropha (Jatropha curcas) Accessions

2020 ◽  
Vol 30 (1) ◽  
pp. 131-141
Author(s):  
Hundessa Fufa ◽  
Jiregna Daksa
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Jatropha Curcas ◽  
Plant Tissue ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Regeneration Medium ◽  
Adventitious Shoot Buds

The present study was undertaken to establish a protocol for in vitro callusing of three Jatropha accessions, namely Metema, Adami Tulu and Shewa Robit from leaf explants. The medium supplemented with combination of 4.44 μM BAP and 4.52 μM 2,4-D resulted in maximum percentage of callus (100%) formed for all accessions. The maximum shoot regeneration (66.67%) from callus with 10.13 number of shoot was obtained from Shewa Robit in MS medum fortified with TDZ (2.27 μM ) and IBA (0.49 μM ). The presence of TDZ in the shoot regeneration medium has greater influence on the induction of adventitious shoot buds, whereas MS supplemented with BAP alone and combination with IBA did not induce shoot regeneration from callus culture. The results obtained in the present study would facilitate the high callus induction and regeneration responses in Jatropha for its improvement using biotechnological tools. Plant Tissue Cult. & Biotech. 30(1): 131-141, 2020 (June)

Adventitious shoot formation from leaf explants of Rhododendron

1992 ◽  
Vol 30 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Giovanni Iapichino ◽  
Steve MeCullech ◽  
Tony H. H. Chen
Keyword(s):  
Leaf Explants ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Shoot Formation

Effect of genotype and medium composition on linseed (Linum usitatissimum) ovary culture

Biologia ◽  
2011 ◽  
Vol 66 (3) ◽  
Author(s):  
Natalija Burbulis ◽  
Aušra Blinstrubienė ◽  
Ramunė Kuprienė
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Linum Usitatissimum ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
Induction Medium ◽  
Ovary Culture ◽  
Adventitious Shoot Formation

AbstractBreeding linseed (Linum usitatissimum L.) using haploid techniques allows breeders to develop new cultivars in a shorter time period. Many research groups successfully created new linseed genotypes through anther culture; however ovary culture has been the subject of only a few earlier studies. In the present study, the effect of genotype and growth regulators combination on callus induction and shoots regeneration in ovary culture of nine commercially important linseed cultivars was investigated. Ovaries were cultured on modified MS medium supplemented with three different combinations of plant growth regulators. Variable callogenic responses were expressed by all of the genotypes tested on different induction media. The results suggested that specific combination of growth regulators for callus induction must be designed for each genotype. Shoot regeneration from ovary derived callus is a critical phase of the whole gynogenetic process. Differences in adventitious shoot formation frequency among genotypes were demonstrated and four responsive genotypes have been selected. Ovary derived callus from cultivar ‘Mikael’ manifested the highest adventitious shoot formation frequency with a high number of shoots per explant. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the genotype. Cultivars ‘Linola’, ‘Mikael’ and ‘Szaphir’ showed the highest shoot regeneration frequency when callus had originated on induction medium supplemented with 2 mg L−1 BAP and 2 mg L−1 NAA, while combination of 1 mg L−1 BAP and 2 mg L−1 IAA promoted shoot formation in ovary-derived callus of ‘Barbara’. The highest rate of shoots per explant has been obtained in second subculture.

Influence of taxonomic relatedness and medium composition on meristematic nodule and adventitious shoot formation in nine pine species

1992 ◽  
Vol 22 (5) ◽  
pp. 750-755 ◽  
Author(s):  
Ben A. Bergmann ◽  
Anne-Marie Stomp
Keyword(s):  
Adventitious Shoot ◽  
Shoot Formation ◽  
Medium Composition ◽  
Meristem Culture ◽  
Indolebutyric Acid ◽  
Meristematic Tissue ◽  
Adventitious Shoot Formation ◽  
Shoot Production ◽  
Half Strength

Embryos of Pinusechinata Mill., Pinustaeda L., Pinusserotina Michx., Pinuseldarica Medwed., Pinuscaribaea Morelet, Pinusoocarpa Scheide, Pinustecunumanii (Schwd.) Equiluz & Perry, Pinusstrobus L., and Pinusradiata D. Don were cultured following the protocol for Pinusradiata to determine if a perpetual meristem culture could be produced. Two subsequent experiments were done that included modifications to the medium's mineral composition, strength (modified LePoivre and Murashige and Skoog at half-strength and full strength), cytokinin concentration (1.0, 2.5, and 5.0 mg/L benzyladenine), and auxin concentration (0 and 0.1 mg/L indolebutyric acid). Pines in subsection Australes performed poorly in culture relative to those species in subsection Oocarpae. Pinusradiata and Pinuseldarica were the only species to produce long-term subculturable meristematic tissue, although shoots were obtained with seven of the species. Half-strength modified LePoivre medium containing 2.5 mg/L benzyladenine but no auxin gave the best results with most species. Significant differences in shoot production were found among Pinusoocarpa provenances.

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