Damage to black locust leaves (Robinia pseudoacacia L.) by the black locust gall midge (Obolodiplosis robiniae) (Haldeman, 1847) in the Włoszczowa-Jędrzejów Protected Landscape Area - research on strengthening and developing knowledge and awareness in environmental education

The prepared article by the team of authors aims to show research in the field of strengthening and developing knowledge and awareness from environmental education in the community of nature conservation services and the academic community. This paper is the result of research conducted in 2014–2015, in the Włoszczowa-Jędrzejów Protected Landscape Area, in the Świętokrzyskie Province. The material for the study was acacia robinia (Robinia pseudoacacia L.) leaves collected on two research areas, differentiated due to the influence of anthropogenic factors. As a result of the study, 5,000 black locust leaves were collected, 65% of which were found to be damaged. Research stands under the influence of strong anthropopressure were characterised by a higher number of lesions on leaves.

Quality Control of Different Types of Honey and Propolis Collected from Romanian Accredited Beekeepers and Consumer’s Risk Assessment

Honey is a natural product recognized and appreciated for its nutritional value and therapeutic potential. However, the quality of bee honey is essential because various contaminants can seriously affect consumers’ health. In the experimental part of the work, we analyzed different types of honey (linden, black locust, rapeseed and multifloral honey) and propolis, which were collected from Romanian accredited beekeepers who placed beehives in two areas characterized by different industrial activity: area 1 (A1) is an area with intense industrial activity, with other industries existing nearby, including a refinery, while area 2 (A2) is entirely devoid of industrial activity, but with moderate agricultural activity. A total of 144 samples were collected, twelve samples for each variety of honey, propolis and soil, corresponding to each area analyzed. In addition, seven heavy metals and three pesticides were tested for in the samples collected. Finally, the correlation between the degree of contamination with soil pollutants and the contamination of the bee products harvested from the analyzed areas was studied. Cadmium, lead, copper, zinc and the sum of DDT metabolites exceeded the maximum allowable levels in honey samples, with differences between different types of honey.

The Properties of Black Locust Robinia pseudoacacia L. to Selectively Accumulate Chemical Elements from Soils of Ecologically Transformed Areas

The black locust Robinia pseudoacacia L. is a common species that originated from North America. In Europe, it is an invasive and introduced plant. Due to its low habitat requirements and ecological plasticity, this species colonizes new anthropogenically transformed areas quickly. This study investigated the chemical composition of selected tissues of Robinia pseudoacacia L. in five various habitats with different levels of anthropopression conditions in southern Poland. The presented research aimed to compare the chemical composition of black locust parts tissues (leaves, branches, and seeds) and the soil under its canopy. To determine the heavy metal contamination and enrichment in soil, the geoaccumulation index, enrichment factor, contamination factor, pollution load index, and potential ecological risk index were calculated. The results showed that all examined soils are considerably or very highly contaminated and the main heavy metals, which pollute the studied samples, are cadmium (1.3–3.91 ppm), lead (78.17–157.99 ppm), and zinc (129.77–543.97 ppm). Conducted research indicates that R. pseudoacacia leaves are the primary carrier of potentially toxic elements. Due to low bioaccumulation factor (BAF) values, it is clear that black locusts do not accumulate contaminants in such amounts that it would pose risk to its use in degraded area reclamation. The obtained results showed that R. pseudoacacia is able to grow in a wide range of habitats and could be applied for greening urban habitats and disturbed ecosystems caused by industry.

Natura 2000 Areas, Road, Railway, Water, and Ecological Networks May Provide Pathways for Biological Invasion: A Country Scale Analysis

Invasive species are a major threat to biodiversity worldwide. Controlling their rapid spread can only be effective if we consider the geographical factors that influence their occurrence. For instance, roads, railway networks, green and blue infrastructure, and elements of ecological networks (e.g., ecological corridors) can facilitate the spread of invasive species. In our study, we mapped the occurrence of five invasive plant taxa (tree of heaven, common milkweed, Russian olive, black locust, and goldenrods) in Hungary, using field photos from the EUROSTAT Land Use and Coverage Area Frame Survey (LUCAS) database from the year 2015. Species point occurrence data were compared with the spatial characteristics of linear transport infrastructure and with the green and blue infrastructure. We found that the occurrence of tree of heaven and Russian olive was strongly related to the road and railway network. The average Euclidean distance of LUCAS points infected with these species from railway embankments and roads was much smaller than that of uninfected points. However, black locust and goldenrods were more common only along the road network. According to our results, the occurrence of some investigated invasive plants was over-represented in the HEN and within Natura 2000 areas of Hungary compared to non-infected points. Our results may provide important information for predicting the rate of invasion and for applying targeted management within the HEN, and Natura 2000 protected areas.

Studies on the Frequency of Attack Produced by Obolodiplosis robiniae Haldeman in Black Locust Trees from Landscaped Spaces of Cluj-Napoca

Obolodiplosis robiniae Haldeman (Diptera: Cecidomyiidae) is a monophagous species, the host plants being species of the genus Robinia. The species is native to the U.S.A., and in Europe, it was first reported in Italy, in 2003, from where it spread to all European countries. In Romania, it was reported in 2007. The attack of this species is produced by the larval stage and manifests leaves. After hatching, the endophytic and gregarious larvae cause changes in the parenchyma structure that lead to the appearance of characteristic galls located at the edge of the leaflet and rolled towards its lower part. In 2021, we monitored the frequency of attacked acacia leaves, the number of galls reported on a leaflet and the number of larvae in a gala in two locations in Cluj-Napoca: on the acacias in the Central Park of the city and on the acacias in some street alignments. In the case of acacias in Central Park, the frequency of attacked fruits was 15%, and in the case of acacias in the alignment, 7%. On the 50 attacked leaves taken from Central Park, there were 932 leaflets, of which 40% are with galls, and the leaves on the alignment had 1010 leaflets, of which 19.2% are with galls. The number of galas/leaflets was between 1 and 5 galas in Central Park, and on alignments between 1 and 4 galas. In both locations, most of the leaflets were with a single gala. The maximum larvae/gala was five specimens in Central Park and three larvae on the leaflets on the alignment.

Epiphytic lichens of woodland habitats in the lower Ticino river valley and in the “Bosco Siro Negri” Integral Nature State Reserve (NW Italy)

Epiphytic lichens were surveyed in the “Bosco Siro Negri” Integral Nature State Reserve (province of Pavia, Lombardy, NW Italy), which hosts a well-preserved fragment of oak-elm floodplain forest (Natura 2000 Habitat 91F0). The core woodland fragment hosted only four species on the tree boles, i.e. Lepraria finkii plus the forest specialists Coenogonium pineti, Diarthonis spadicea, and Opegrapha vermicellifera. An additional eight nitro- and photophytic species were recorded on the highest twigs of the canopy. The whole Reserve, including the neighbouring degraded woodlands and poplar plantations, hosted 27 epiphytic lichen taxa. The lower Ticino River valley between Vigevano and Pavia, in which the Reserve is located, was surveyed for epiphytic lichens in an additional 45 sites, including 15 wellpreserved oak-elm/hornbeam woodlands (Habitat 91F0), 15 degraded broadleaved woodlands with high occurrence of black locust and 15 poplar plantations. Overall, 32 species were recorded. Well-preserved woodlands had a lower species richness, but they hosted forest specialists not occurring in the other two habitats. Graphis pulverulenta and Lecania cyrtellina are new to Lombardy. Anisomeridium polypori, Diarthonis spadicea, Lecanora expallens and Pseudoschismatomma rufescens are reported from Lombardy for the second time.

What Could Arrest an Eriophyoid Mite on a Plant? The Case of Aculops allotrichus from the Black Locust Tree

Aculops allotrichus is a vagrant eriophyoid that lives gregariously on the leaves of the black locust tree. This study demonstrated that conspecifics can have a significant impact on A. allotrichus females on unprofitable, old black locust leaves and can arrest them on those leaves. The effect was more pronounced in females that were exposed to artificially injured individuals than to intact ones. They not only prolonged their sojourn on leaf discs with pierced conspecifics, but also preferred the leaf disc halves with damaged individuals to clean ones. Aculops allotrichus is the first described herbivore in which artificially injured conspecifics, instead of causing alarm, keep the foraging individuals within a risky patch. Other objects, such as artificially injured or intact heterospecifics, pollen or sand, were irrelevant to the eriophyoid females on old leaf patches. In tests with old leaves of maple, magnolia and hard kiwi vine, the females postponed their movement from non-host leaf discs, which suggests that they may need more time to recognise and evaluate unfamiliar plants than familiar ones.

Non-native plant drives the spatial dynamics of its herbivores: the case of black locust (Robinia pseudoacacia) in Europe

Non-native plants typically benefit from enemy release following their naturalization in non-native habitats. However, over time, herbivorous insects specializing on such plants may invade from the native range and thereby diminish the benefits of enemy release that these plants may experience. In this study, we compare rates of invasion spread across Europe of three North American insect folivores: the Lepidoptera leaf miners Macrosaccus robiniella and Parectopa robiniella, and the gall midge Obolodiplosis robiniae, that specialize on Robinia pseudoacacia. This tree species is one of the most widespread non-native trees in Europe. We find that spread rates vary among the three species and that some of this variation can be explained by differences in their life history traits. We also report that geographical variation in spread rates are influenced by distribution of Robinia pseudoacacia, human population and temperature, though Robinia pseudoacacia occurrence had the greatest influence. The importance of host tree occurrence on invasion speed can be explained by the general importance of hosts on the population growth and spread of invading species.