Injectable Nucleus Pulposus Derived-ECM Hydrogel Functionalised with Chondroitin Sulfate for Intervertebral Disc Regeneration

2020 ◽  
Author(s):  
Chiara Borrell ◽  
Conor T. Buckley
Keyword(s):  
Intervertebral Disc ◽  
Chondroitin Sulfate ◽  
Nucleus Pulposus ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration

P34. Tissue-engineered Composite of Three Dimensional PLGA Scaffold Seeded with Nucleus Pulposus Cells for Intervertebral Disc Regeneration

2008 ◽  
Vol 8 (5) ◽  
pp. 117S-119S ◽  
Author(s):  
Chao Zhang ◽  
Ruan Dike ◽  
Rongfeng Zhang ◽  
Shenguo Wang ◽  
Fei Yang
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Three Dimensional ◽  
Plga Scaffold ◽  
Nucleus Pulposus Cells ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration

Effect of perfluorotributylamine-enriched alginate on nucleus pulposus cell: Implications for intervertebral disc regeneration

Biomaterials ◽  
2016 ◽  
Vol 82 ◽  
pp. 34-47 ◽  
Author(s):  
Zhen Sun ◽  
Beier Luo ◽  
Zhongyang Liu ◽  
Liangliang Huang ◽  
Bing Liu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cell ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration

scholarly journals Generation of human nucleus pulposus cells from stem cells: First steps towards intervertebral disc regeneration.

2016 ◽  
Vol 24 ◽  
pp. S11-S12 ◽  
Author(s):  
P. Colombier ◽  
J. Clouet ◽  
C. Chariau ◽  
C. Chedeville ◽  
J. Lesoeur ◽  
...  
Keyword(s):  
Stem Cells ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration

Understanding the native nucleus pulposus cell phenotype has important implications for intervertebral disc regeneration strategies

2013 ◽  
Vol 8 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Francesca E Ludwinski ◽  
Kanna Gnanalingham ◽  
Stephen M Richardson ◽  
Judith A Hoyland
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cell ◽  
Cell Phenotype ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration ◽  
Regeneration Strategies

Thermosensitive injectable decellularized nucleus pulposus hydrogel as an ideal biomaterial for nucleus pulposus regeneration

2020 ◽  
Vol 35 (2) ◽  
pp. 182-192 ◽  
Author(s):  
Lei Yu ◽  
Zi-Jie Sun ◽  
Quan-Chang Tan ◽  
Shuang Wang ◽  
Wei-Heng Wang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Three Dimensional ◽  
Uniform Grid ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration ◽  
Low Toxicity ◽  
Three Dimensional Culture

Extracellular matrix loss is one of the early manifestations of intervertebral disc degeneration. Stem cell-based tissue engineering creates an appropriate microenvironment for long term cell survival, promising for NP regeneration. We created a decellularized nucleus pulposus hydrogel (DNPH) from fresh bovine nucleus pulposus. Decellularization removed NP cells effectively, while highly preserving their structures and major biochemical components, such as glycosaminoglycan and collagen II. DNPH could be gelled as a uniform grid structure in situ at 37°C for 30 min. Adding adipose marrow-derived mesenchymal stem cells into the hydrogel for three-dimensional culture resulted in good bioactivity and biocompatibility in vitro. Meanwhile, NP-related gene expression significantly increased without the addition of exogenous biological factors. In summary, the thermosensitive and injectable hydrogel, which has low toxicity and inducible differentiation, could serve as a bio-scaffold, bio-carrier, and three-dimensional culture system. Therefore, DNPH has an outstanding potential for intervertebral disc regeneration.

Potential of co-culture of nucleus pulposus mesenchymal stem cells and nucleus pulposus cells in hyperosmotic microenvironment for intervertebral disc regeneration

2013 ◽  
Vol 37 (8) ◽  
pp. 826-834 ◽  
Author(s):  
Yi-Qing Tao ◽  
Cheng-Zhen Liang ◽  
Hao Li ◽  
Yu-Jie Zhang ◽  
Fang-Cai Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration
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