scholarly journals An ATP-Regulated and pH-Sensitive Inwardly Rectifying K+ Channel in Cultured Human Proximal Tubule Cells.

2001 ◽  
Vol 51 (4) ◽  
pp. 523-530 ◽  
Author(s):  
Kazuyoshi Nakamura ◽  
Junko Hirano ◽  
Manabu Kubokawa
Keyword(s):  
Proximal Tubule ◽  
Ph Sensitive ◽  
K Channel ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Inwardly Rectifying ◽  
Human Proximal Tubule

Protein kinase G activates inwardly rectifying K+ channel in cultured human proximal tubule cells

2002 ◽  
Vol 283 (4) ◽  
pp. F784-F791 ◽  
Author(s):  
Kazuyoshi Nakamura ◽  
Junko Hirano ◽  
Shun-Ichi Itazawa ◽  
Manabu Kubokawa
Keyword(s):  
Proximal Tubule ◽  
Specific Inhibitor ◽  
K Channel ◽  
Channel Activity ◽  
Patch Clamp Technique ◽  
Proximal Tubule Cells ◽  
Bath Application ◽  
Tubule Cells ◽  
Inwardly Rectifying ◽  
Human Proximal Tubule

An ATP-regulated inwardly rectifying K+ channel, whose activity is enhanced by PKA, is present in the plasma membrane of cultured human proximal tubule cells. In this study, we investigated the effects of PKG on this K+ channel, using the patch-clamp technique. In cell-attached patches, bath application of a membrane-permeant cGMP analog, 8-bromoguanosine 3′,5′-monophosphate (8-BrcGMP; 100 μM), stimulated channel activity, whereas application of a PKG-specific inhibitor, KT-5823 (1 μM), reduced the activity. Channel activation induced by 8-BrcGMP was observed even in the presence of a PKA-specific inhibitor, KT-5720 (500 nM), which was abolished by KT-5823. Direct effects of cGMP and PKG were examined with inside-out patches in the presence of 1 mM MgATP. Although cytoplasmic cGMP (100 μM) alone had little effect on channel activity, subsequent addition of PKG (500 U/ml) enhanced it. Furthermore, bath application of atrial natriuretic peptide (ANP; 20 nM) in cell-attached patches stimulated channel activity, which was blocked by KT-5823. In conclusion, cGMP/PKG-dependent processes participate in activating the ATP-regulated K+ channel and producing the stimulatory effect of ANP on channel activity.

scholarly journals Activation of Inwardly Rectifying K+ Channel in OK Proximal Tubule Cells Involves cGMP-Dependent Phosphorylation Process.

1998 ◽  
Vol 48 (6) ◽  
pp. 467-476 ◽  
Author(s):  
Manabu KUBOKAWA ◽  
Shigeyuki NAKAYA ◽  
Yoshichika YOSHIOKA ◽  
Kazuyoshi NAKAMURA ◽  
Fumio SATO ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
K Channel ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Inwardly Rectifying

scholarly journals Involvement of Endogenous Nitric Oxide in the Regulation of K+ Channel Activity in Cultured Human Proximal Tubule Cells

2006 ◽  
Vol 56 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Kazuyoshi Nakamura ◽  
Wataru Habano ◽  
Toshiyuki Kojo ◽  
You Komagiri ◽  
Takahiro Kubota ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Proximal Tubule ◽  
K Channel ◽  
Channel Activity ◽  
Proximal Tubule Cells ◽  
Endogenous Nitric Oxide ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals Electrophysiology and ultrastructure of cultured human proximal tubule cells

1988 ◽  
Vol 33 (2) ◽  
pp. 508-516 ◽  
Author(s):  
John G. Blackburn ◽  
Debra J. Hazen-Martin ◽  
Carol J. Detrisac ◽  
Donald A. Sens
Keyword(s):  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals Uremic Toxins Induce ET-1 Release by Human Proximal Tubule Cells, which Regulates Organic Cation Uptake Time-Dependently

Cells ◽  
2015 ◽  
Vol 4 (3) ◽  
pp. 234-252 ◽  
Author(s):  
Carolien Schophuizen ◽  
Joost Hoenderop ◽  
Rosalinde Masereeuw ◽  
Lambert Heuvel
Keyword(s):  
Proximal Tubule ◽  
Organic Cation ◽  
Uremic Toxins ◽  
Proximal Tubule Cells ◽  
Cation Uptake ◽  
Tubule Cells ◽  
Human Proximal Tubule
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