scholarly journals Electrophoretic Separation of Human Skeletal Muscle Myosin Heavy Chain Isoforms: The Importance of Reducing Agents

2006 ◽  
Vol 56 (5) ◽  
pp. 355-360 ◽  
Author(s):  
Tertius Abraham Kohn ◽  
Kathryn Helen Myburgh
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Reducing Agents ◽  
Myosin Heavy Chain Isoforms ◽  
Electrophoretic Separation ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

Improving human skeletal muscle myosin heavy chain fiber typing efficiency

2016 ◽  
Vol 37 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Kevin A. Murach ◽  
James R. Bagley ◽  
Kathryn A. McLeland ◽  
Jose A. Arevalo ◽  
Anthony B. Ciccone ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

scholarly journals Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms

1993 ◽  
Vol 75 (5) ◽  
pp. 2337-2340 ◽  
Author(s):  
R. J. Talmadge ◽  
R. R. Roy
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Medial Gastrocnemius ◽  
New Technique ◽  
Blue Staining ◽  
Electrophoretic Separation ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

A new technique for the sodium dodecyl sulfate-polyacrylamide gel electrophoretic separation of rat skeletal muscle myosin heavy-chain (MHC) isoforms is presented. This technique allows for the separation of the four identified MHC isoforms known to be present in adult rat skeletal muscle. These types of MHC are commonly called I, IIa, IIx or IId, and IIb. The procedure can be performed using minigel electrophoresis systems and does not involve preparation of gradient-separating gels or the use of special cooling devices. The procedure accommodates both silver and Coomasie Blue staining. Thus the procedure is simple to perform and highly repeatable, providing high-resolution separation of MHC protein isoforms. The percent composition of the four adult MHCs in rat soleus, medial gastrocnemius, diaphragm, and levator ani muscles by use of this procedure and Coomasie Blue staining is similar to that previously reported. This new technique provides a novel and easy-to-perform method for the separation of rat skeletal muscle MHC isoforms.

HUMAN SKELETAL MUSCLE MYOSIN HEAVY CHAIN EXPRESSION IN RESPONSE TO STRENGTH TRAINING

2002 ◽  
Vol 34 (5) ◽  
pp. S120
Author(s):  
Y Liu ◽  
J M. Steinacker ◽  
C Baur ◽  
L Wang ◽  
A Schlumberger ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

Isolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms

1998 ◽  
Vol 29 (2) ◽  
pp. 91-97 ◽  
Author(s):  
R. Davoli ◽  
P. Zambonelli ◽  
D. Bigi ◽  
L. Fontanesi ◽  
V. Russo
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Myosin Heavy Chain Isoforms ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

Effect of Dietary Creatine on Skeletal Muscle Myosin Heavy Chain Isoform Expression in an Animal Model of Uremia

2004 ◽  
Vol 96 (4) ◽  
pp. e103-e110 ◽  
Author(s):  
Youri E.C. Taes ◽  
Marijn Speeckaert ◽  
Evelien Bauwens ◽  
Marc R. De Buyzere ◽  
Johan Libbrecht ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Animal Model ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Skeletal Muscle Myosin ◽  
Myosin Heavy Chain Isoform ◽  
Isoform Expression ◽  
Muscle Myosin
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