Improving human skeletal muscle myosin heavy chain fiber typing efficiency

2016 ◽  
Vol 37 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Kevin A. Murach ◽  
James R. Bagley ◽  
Kathryn A. McLeland ◽  
Jose A. Arevalo ◽  
Anthony B. Ciccone ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

HUMAN SKELETAL MUSCLE MYOSIN HEAVY CHAIN EXPRESSION IN RESPONSE TO STRENGTH TRAINING

2002 ◽  
Vol 34 (5) ◽  
pp. S120
Author(s):  
Y Liu ◽  
J M. Steinacker ◽  
C Baur ◽  
L Wang ◽  
A Schlumberger ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Myosin ◽  
Muscle Myosin

Effect of Dietary Creatine on Skeletal Muscle Myosin Heavy Chain Isoform Expression in an Animal Model of Uremia

2004 ◽  
Vol 96 (4) ◽  
pp. e103-e110 ◽  
Author(s):  
Youri E.C. Taes ◽  
Marijn Speeckaert ◽  
Evelien Bauwens ◽  
Marc R. De Buyzere ◽  
Johan Libbrecht ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Animal Model ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Skeletal Muscle Myosin ◽  
Myosin Heavy Chain Isoform ◽  
Isoform Expression ◽  
Muscle Myosin

scholarly journals Isolation of human skeletal muscle myosin heavy chain and actin for measurement of fractional synthesis rates

1998 ◽  
Vol 275 (6) ◽  
pp. E1092-E1099 ◽  
Author(s):  
D. L. Hasten ◽  
G. S. Morris ◽  
S. Ramanadham ◽  
K. E. Yarasheski
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Human Skeletal Muscle ◽  
Sodium Dodecyl ◽  
Synthesis Rate ◽  
Simple Method ◽  
Skeletal Muscle Myosin ◽  
Fractional Synthesis

Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), we have developed a simple method to isolate myosin heavy chain (MHC) and actin from small (60–80 mg) human skeletal muscle samples for the determination of their fractional synthesis rates. The amounts of MHC and actin isolated are adequate for the quantification of [13C]leucine abundance by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Fractional synthesis rates of mixed muscle protein (MMP), MHC, and actin were determined in six healthy young subjects (27 ± 1 yr) after they received a 14-h intravenous infusion (prime = 7.58 μmol/kg body wt, constant infusion = 7.58 μmol ⋅ kg body wt−1 ⋅ h−1) of [1-13C]leucine. The fractional synthesis rates of MMP, MHC, and actin were found to be 0.0468 ± 0.0048, 0.0376 ± 0.0033, and 0.0754 ± 0.0078%/h, respectively. Overall, the synthesis rate of MHC was 20% lower ( P = 0.012), and the synthesis rate of actin was 61% higher ( P = 0.060, not significant) than the MMP synthesis rate. The isolation of these proteins for isotope abundance analysis by GC-C-IRMS provides important information about the synthesis rates of these specific contractile proteins, as opposed to the more general information provided by the determination of MMP synthesis rates.

Changes in skeletal muscle myosin heavy chain isoform content during congestive heart failure

2002 ◽  
Vol 87 (2) ◽  
pp. 182-186 ◽  
Author(s):  
Espen Spangenburg ◽  
Robert Talmadge ◽  
Timothy Musch ◽  
Pfeifer P. ◽  
Richard McAllister ◽  
...  
Keyword(s):  
Heart Failure ◽  
Skeletal Muscle ◽  
Congestive Heart Failure ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Skeletal Muscle Myosin ◽  
Myosin Heavy Chain Isoform ◽  
Muscle Myosin
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