Regulating Cysteine Protease Activity: Essential Role of Protease Inhibitors as Guardians and Regulators

2005 ◽  
Vol 2 (4) ◽  
pp. 283-297 ◽  
Author(s):  
Boris Turk ◽  
Dusan Turk ◽  
Guy Salvesen
Keyword(s):  
Protease Inhibitors ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Essential Role ◽  
Cysteine Protease Activity

scholarly journals Structural Basis for the Regulation of Cysteine-Protease Activity by a New Class of Protease Inhibitors in Plasmodium

Structure ◽  
2011 ◽  
Vol 19 (7) ◽  
pp. 919-929 ◽  
Author(s):  
Guido Hansen ◽  
Anna Heitmann ◽  
Tina Witt ◽  
Honglin Li ◽  
Hualiang Jiang ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Structural Basis ◽  
New Class ◽  
Cysteine Protease Activity

scholarly journals Cysteine protease activity in the wall of abdominal aortic aneurysms

2007 ◽  
Vol 46 (6) ◽  
pp. 1260-1266 ◽  
Author(s):  
Said Abisi ◽  
Kevin G. Burnand ◽  
Matthew Waltham ◽  
Julia Humphries ◽  
Peter R. Taylor ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Abdominal Aortic Aneurysms ◽  
Aortic Aneurysms ◽  
Cysteine Protease Activity ◽  
Abdominal Aortic

scholarly journals The M Protein Is Dispensable for Maturation of Streptococcal Cysteine Protease SpeB

2005 ◽  
Vol 73 (2) ◽  
pp. 859-864 ◽  
Author(s):  
Björn Zimmerlein ◽  
Hae-Sun Park ◽  
Shaoying Li ◽  
Andreas Podbielski ◽  
P. Patrick Cleary
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Active Form ◽  
Surface Protein ◽  
M Protein ◽  
Cysteine Protease Activity ◽  
Group A ◽  
Streptococcal Pyrogenic Exotoxin B ◽  
Major Surface ◽  
Culture Supernatants

ABSTRACT The streptococcal pyrogenic exotoxin B (SpeB) is an important virulence factor of group A streptococci (GAS) with cysteine protease activity. Maturation of SpeB to a proteolytically active form was suggested to be dependent on cell-wall-anchored M1 protein, the major surface protein of GAS (M. Collin and A. Olsén, Mol. Microbiol. 36:1306-1318, 2000). Collin and Olsén showed that mutant GAS strains expressing truncated M protein secrete a conformationally different form of unprocessed SpeB with no proteolytic activity. Alternatively, we hypothesized that a truncated M protein may interfere with processing of this secreted protease, and therefore we tested cysteine protease activity in genetically defined mutant strains that express either no M protein or membrane-anchored M protein with an in-frame deletion of the AB repeat region. Measurements of SpeB activity by cleavage of a substrate n-benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride showed that the proteolytic activities in culture supernatants of both mutants were similar to those from the wild-type strain. In addition, Western blot analysis of culture supernatants showed that SpeB expression and processing to a mature form was unaffected by either deletion mutation. Therefore, we conclude that M protein is not required for maturation of the streptococcal cysteine protease SpeB.

scholarly journals Epicutaneous Administration of Papain Induces IgE and IgG Responses in a Cysteine Protease Activity-Dependent Manner

2014 ◽  
Vol 63 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Hideo Iida ◽  
Toshiro Takai ◽  
Yusuke Hirasawa ◽  
Seiji Kamijo ◽  
Sakiko Shimura ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Dependent Manner ◽  
Cysteine Protease Activity ◽  
Activity Dependent

Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes

2007 ◽  
Vol 3 (10) ◽  
pp. 668-677 ◽  
Author(s):  
Galia Blum ◽  
Georges von Degenfeld ◽  
Milton J Merchant ◽  
Helen M Blau ◽  
Matthew Bogyo
Keyword(s):  
Optical Imaging ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Cysteine Protease Activity
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