Cladistic Analysis of Restriction Site Variation within the Chloroplast DNA Inverted Repeat Region of Selected Hamamelididae

Tribal and subtribal boundaries within the Brassicaceae are often artificially drawn. Most of the currently recognized tribes and subtribes are separated by only a few morphological characters. Furthermore, these characters are variable even within genera, or they conflict with one another in their distribution patterns, resulting in controversial classification systems. Therefore, tribes and subtribes as traditionally delimited may not reflect natural groups. Up to now no cladistic analysis has been performed in tribe Lepidieae. In the current study, we have analyzed restriction site variation of chloroplast DNA among 41 species representing 19 genera to get insights into phylogenetic relationships within the Lepidieae. Phylogenetic lineages derived from our chloroplast data were critically compared with the traditional concepts. Our chloroplast DNA phylogeny most easily supports the classification of genera in the system of Hayek with some modifications.

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Phylogenetic implications of chloroplast DNA restriction site variation in subtribes Raphaninae and Cakilinae (Brassicaceae, tribe Brassiceae)

Canadian Journal of Botany ◽

10.1139/b97-107 ◽

1997 ◽

Vol 75 (6) ◽

pp. 960-973 ◽

Cited By ~ 57

Author(s):

S. I. Warwick ◽

L. D. Black

Keyword(s):

Chloroplast Dna ◽

Restriction Site ◽

Cladistic Analysis ◽

Raphanus Raphanistrum ◽

Restriction Site Variation ◽

Phylogenetic Implications ◽

Site Variation ◽

Subtribe Brassicinae ◽

Dna Restriction ◽

Rapistrum Rugosum

Chloroplast DNA restriction site data was used to assess relationships among 41 taxa of the subtribes Raphaninae and Cakilinae (tribe Brassiceae, Brassicaceae). A total of 456 restriction site mutations was observed, with 237 (52%) being phylogenetically informative. Cladistic analysis, based on Wagner parsimony analysis, indicated four major clades: (i) CAKILE, (ii) CRAMBE, (iii) NIGRA lineage, and (iv) RAPA-OLERACEA lineage. The CAKILE clade was divided into two lineages: (i) Cakile, Erucaria (including Reboudia), and Didesmus (previously assigned by Schulz to the Raphaninae) and (ii) Crambella (previously assigned by Schulz to the Raphaninae). The Raphaninae as currently circumscribed is polyphyletic in origin. The cpDNA data supported separate subtribal status for Crambe (Raphaninae) and indicated two major lineages corresponding to sect. Dendrocrambe and the combined sections Crambe and Leptocrambe. Enarthrocarpus arcuatus, Enarthrocarpus lyratus, Morisia monanthos, Raphanus raphanistrum, Raphanus sativus, and Rapistrum perenne were included in the RAPA-OLERACEA lineage of subtribe Brassicinae. Ceratocnemum rapistroides, Cordylocarpus muricatus, Guiraoa arvensis, Hemicrambe fruticulosa, Kremeriella cordylocarpus, Muricaria prostrata, Otocarpus virgatus, Raffenaldia primuloides, and Rapistrum rugosum were included in the NIGRA lineage of subtribe Brassicinae. Key words: Brassicinae, Raphaninae, Cakilinae, chloroplast DNA restriction site variation, molecular systematics.

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