scholarly journals Discussion of the Effect of Positional Tolerance on other Tolerances

2017 ◽  
Author(s):  
Shu-ping Lv
Keyword(s):  
Positional Tolerance

scholarly journals Protein tolerance to random circular permutation correlates with thermostability and local energetics of residue-residue contacts

2019 ◽  
Vol 32 (11) ◽  
pp. 489-501
Author(s):  
Joshua T Atkinson ◽  
Alicia M Jones ◽  
Vikas Nanda ◽  
Jonathan J Silberg
Keyword(s):  
Melting Temperature ◽  
Primary Structure ◽  
Adenylate Kinase ◽  
Conformational Flexibility ◽  
Circular Permutation ◽  
Binding Domain ◽  
Enzyme Family ◽  
Residue Contacts ◽  
Positional Tolerance ◽  
New Protein

Abstract Adenylate kinase (AK) orthologs with a range of thermostabilities were subjected to random circular permutation, and deep mutational scanning was used to evaluate where new protein termini were nondisruptive to activity. The fraction of circularly permuted variants that retained function in each library correlated with AK thermostability. In addition, analysis of the positional tolerance to new termini, which increase local conformational flexibility, showed that bonds were either functionally sensitive to cleavage across all homologs, differentially sensitive, or uniformly tolerant. The mobile AMP-binding domain, which displays the highest calculated contact energies, presented the greatest tolerance to new termini across all AKs. In contrast, retention of function in the lid and core domains was more dependent upon AK melting temperature. These results show that family permutation profiling identifies primary structure that has been selected by evolution for dynamics that are critical to activity within an enzyme family. These findings also illustrate how deep mutational scanning can be applied to protein homologs in parallel to differentiate how topology, stability, and local energetics govern mutational tolerance.

scholarly journals Explicitly representing the semantics of composite positional tolerance for patterns of holes

2016 ◽  
Vol 90 (5-8) ◽  
pp. 2121-2137 ◽  
Author(s):  
Yuchu Qin ◽  
Wenlong Lu ◽  
Qunfen Qi ◽  
Tukun Li ◽  
Meifa Huang ◽  
...  
Keyword(s):  
Positional Tolerance

An Approach for Generating Variational Geometry of a Pattern of Holes With Composite Positional Tolerances

2002 ◽  
Author(s):  
Y. S. Liu ◽  
S. M. Gao
Keyword(s):  
Mathematical Models ◽  
Geometric Modeling ◽  
Mechanical Design ◽  
Cad Systems ◽  
Computer Aided ◽  
Cad Cam ◽  
Positional Tolerance

In most present CAD systems, the accurate geometry representation of a part is given but the tolerance is just a text attribute without semantics. It hampers the integration of CAD/CAM. In last two decades, much research has been conducted on computer aided tolerancing. And many approaches to generation of variational geometry for various kinds of features have been given. However how to generate the variational geometry of a pattern of holes (POH) is not well studied yet although a POH is one of the most widely-used features in mechanical design. Based on DOF, an approach is proposed for generating variational geometry of a POH with composite positional tolerance in this paper. First, the limitation conditions for variations of a POH are given. Second, the mathematical models of translational and rotational variations are systematically derived. Then the algorithm for generating variational geometry of a POH is given. At last the proposed approach has been implemented with Visual C++ based on the geometric modeling kernel ACIS5.0.

An Improved Features of Health Screening Test System for Malaysian Social Security Organisation (SOCSO) Programme

2017 ◽  
Vol 6 (2) ◽  
pp. 473
Author(s):  
A. R. Abdullah ◽  
A. R. Syafeeza ◽  
M. A. U. C. Mood ◽  
T. N. S. T. Zawawi ◽  
A. R. Munzier
Keyword(s):  
Social Security ◽  
Screening Test ◽  
Time Frame ◽  
Test System ◽  
Health Screening ◽  
Functional Capacity Evaluation ◽  
Physical Evaluation ◽  
Time Motion ◽  
Proposed Model ◽  
Positional Tolerance

The purpose of this paper is to improve the features of Health Screening Test System (HSTS) on Social Security Organization (SOCSO) program as physical evaluation for musculoskeletal disable workers (MSDs). SOCSO existing functional testing system are not suitable because of the evaluation was recorded manually peg board too high for Asian people. The occupational therapist whose involve in all the procedures is just doing the judgment in times to determine the capability of the patients. The functional capacity evaluation (FCE) technique is based on the functional range of motion evaluation that consist of positional tolerance respecting to time-motion testing on HSTS peg board and it is by referring to the original work movement. The main features of HSTS are able to measure speed, acceleration and evaluation of SOCSO’s patients for returning to work based on SOCSO’s requirement. In order to validate the accuracy of the proposed model, HSTS is used to evaluate the patient’s positional tolerance and then the result would be compare over the time frame which is Method Time Measurement (MTM) standard. This method is able to provide information and feedback for therapies as a status of patients. It is found that the proposed model is superior in getting the accurate time test for patient’s movements besides practicable and suitable for physical evaluation on MSD patients.

scholarly journals Family permutation profiling identifies a dynamic protein domain as functionally tolerant to increased conformational entropy

2019 ◽  
Author(s):  
Joshua T. Atkinson ◽  
Alicia M. Jones ◽  
Vikas Nanda ◽  
Jonathan J. Silberg
Keyword(s):  
Enzymatic Activity ◽  
Melting Temperature ◽  
Primary Structure ◽  
Adenylate Kinase ◽  
Protein Domain ◽  
Conformational Entropy ◽  
Peptide Bonds ◽  
Positional Tolerance ◽  
And Function ◽  
New Protein

ABSTRACTTo investigate whether adenylate kinase (AK) homologs differ in their functional tolerance to mutational lesions that alter dynamics, we subjected three homologs having a range of thermostabilities to random circular permutation and evaluated where new protein termini were non-disruptive to activity using a cellular selection and deep mutational scanning. Analysis of the positional tolerance to new termini, which increase local conformational entropy by breaking peptide bonds, showed that bonds were either functionally sensitive to cleavage across all three homologs, differentially sensitive, or uniformly tolerant. The mobile AMP binding domain, which displays the highest calculated contact energies (frustration), presented the greatest tolerance to new termini across all AKs. In contrast, retention of function in the lid and core domains was more dependent upon AK melting temperature. Thus, regions of high energetic frustration tolerated increases in conformational entropy in a manner that was less dependent on thermostability than regions of lower frustration. Our results suggest that family permutation profiling identifies primary structure that has been selected by evolution for high frustration that is critical to enzymatic activity. They also illustrate how deep mutational scanning can be applied to protein homologs in parallel to learn how topology and function govern mutational tolerance.

scholarly journals POSITIONAL TOLERANCE TRAINING AIDS

1961 ◽  
Author(s):  
P A Nicovich ◽  
R O Dell
Keyword(s):  
Training Aids ◽  
Positional Tolerance
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